P5306
Papain from papaya latex
lyophilized powder, aseptically filled
Synonym(s):
Papainase
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biological source
papaya (latex)
Quality Level
sterility
aseptically filled
form
lyophilized powder
specific activity
≥8.0 units/mg protein
mol wt
23.4 kDa
solubility
H2O: soluble 1.2 mg/mL
storage temp.
−20°C
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1 of 4
This Item | P4762 | P3125 | P3375 |
|---|---|---|---|
| biological source papaya (latex) | biological source papaya (latex) | biological source - | biological source - |
| specific activity ≥8.0 units/mg protein | specific activity ≥10 units/mg protein | specific activity ≥16 units/mg protein | specific activity 1.5-10 units/mg solid |
| form lyophilized powder | form lyophilized powder | form buffered aqueous suspension | form crude powder |
| solubility H2O: soluble 1.2 mg/mL | solubility - | solubility - | solubility - |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
| mol wt 23.4 kDa | mol wt 23.4 kDa | mol wt 23.4 kDa | mol wt 23.4 kDa |
General description
Papain is a thermostable enzyme and becomes inactive at 55°C. It has two domains and the catalytic region is present at the domain interface.[1] The cysteine 25 in the active site performs the proteolysis by nucleophilic attack on the carbonyl carbon of a peptides. Papain has wide industrial applications in food and pharmaceutical industry.[2]
Application
Papain is used in dissecting solutions. It is used to produce Fab fragments of antibodies [3]. It is used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. Papain has been used to isolate morphologically intact cortical neurons from postnatal rats. Limited papain digestion is used for structural studies of enzymes and other proteins. In addition, papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens. Papain is useful for platelet serology studies. Papain has also been used in the enzymatic synthesis of amino acids, peptides, and other molecules. Product P5306 is provided as a lyophilized powder that is aseptically filled. It has been used to digest mouse retinas to study the RAGE axis in nonproliferative diabetic retinopathy (NPDR)[4].
The product from Sigma has been used in the digestion of extracellular matrix (ECM).[4] It has also been used with other proteolytic enzymes for in situ protein degradation to produce crystals suitable for structure determination.[5]
Used to produce Fab fragments of antibodies. Also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues.
Biochem/physiol Actions
A cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
pH optimum 6.0-7.0
Also hydrolyzes esters and amides.
pH optimum 6.0-7.0
Also hydrolyzes esters and amides.
Papain papaya latex has antifungal activity against C. albicans. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.
Preparation Note
Prepared from P 4762
Other Notes
One unit will hydrolyze 1.0 μmole of BAEE per minute at pH 6.2 at 25 deg C.
View more information on papain at www.sigma-aldrich.com/enzymeexplorer.
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Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Improving thermostability of papain through structure-based protein engineering
Choudhury D, et al.
Protein engineering, design & selection : PEDS, 23(6), 457-467 (2010)
Clara Malo et al.
Andrologia, 53(11), e14224-e14224 (2021-09-09)
This study evaluated the effects of freezing extenders, cryoprotectants and their concentrations, presence of Orvus Es Paste and freezing rates for cryopreserving dromedary camel sperm. Semen (five males; 2 ejaculates/male) was frozen in one of the following extenders (Green Buffer®
Gaetano R Barile et al.
Investigative ophthalmology & visual science, 46(8), 2916-2924 (2005-07-27)
The receptor for advanced glycation end products (AGEs) has been implicated in the pathogenesis of diabetic complications. This study was conducted to characterize the role of the RAGE axis in a murine model of nonproliferative diabetic retinopathy (NPDR). The retinas
Tsunghan Hsieh et al.
Frontiers in immunology, 13, 901030-901030 (2022-07-16)
Clonal expansion and differentiation of various T helper subsets, such as Th1, Th2, and Th17 cells, depend on a complex of transcription factors, IRF4 and a BATF-containing AP-1 heterodimer. A major BATF heterodimeric partner, JunB, regulates Th17 differentiation, but the
Amy Wernimont et al.
PloS one, 4(4), e5094-e5094 (2009-04-09)
For every 100 purified proteins that enter crystallization trials, an average of 30 form crystals, and among these only 13-15 crystallize in a form that enables structure determination. In 2007, Dong et al reported that the addition of trace amounts
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Uncover properties and applications of the cysteine protease papain and find inhibitors, substrates, and other papain products.
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