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1.05287

Xylose Lysine Deoxycholate agar

GranuCult® prime, according to ISO 6579, ISO 19250, APHA, USDA-FSIS, granular, for Salmonella spp., pkg of 500 g

Synonym(s):

XLD agar

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About This Item

NACRES:
NA.74
UNSPSC Code:
41106213
Manufacturer/tradename:
GranuCult® prime
Agency:
APHA, ISO 19250, USDA-FSIS, according to ISO 6579
Ph:
7.2-7.6 (25 °C, 55 g/L in H2O)
Form:
granular
Suitability:
Salmonella spp.
Application(s):
food and beverages
microbiology
This item can only be ordered on the Merck Millipore website.
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Quality Segment

200

agency

APHA, ISO 19250, USDA-FSIS, according to ISO 6579

sterility

non-sterile

form

granular

packaging

pkg of 500 g

manufacturer/tradename

GranuCult® prime

technique(s)

microbiological culture: suitable

pH

7.2-7.6 (25 °C, 55 g/L in H2O)

solubility

55 g/L

application(s)

food and beverages
microbiology

storage temp.

15-25°C

suitability

Salmonella spp.

General description

GranuCult® Xylose Lysine Deoxycholate agar is designed for the isolation and differentiation of pathogenic Enterobacteriaceae, especially of Shigella and Salmonella species. Degradation of xylose, lactose, and sucrose to acid causes phenol red to change its color to yellow. Production of hydrogen sulfide is indicated by thiosulfate and iron (III) salt, which react to form a precipitate of black iron sulfide in the colonies. Bacteria that decarboxylate lysine to cadaverine can be recognized by the appearance of a purple coloration around the colonies due to an increase in pH. These reactions can proceed simultaneously or successively, causing the pH indicator to exhibit various shades of color, or it may change its color from yellow to red on prolonged incubation. The culture medium is moderately selective, thus the growth of unwanted organisms is suppressed, while sought bacteria can be tentatively grouped by reading the net effect of carbohydrate dissimilation, lysine decarboxylation, and the formation of hydrogen sulfide leading to black colonies. Agar is the solidifying agent.

Application

GranuCult® Xylose Lysine Deoxycholate agar is suitable for the isolation and differentiation of pathogenic Enterobacteriaceae, especially of Shigella and Salmonella species from food and animal feed, water, and other materials.

Analysis Note

Appearance (clearness): clear to slightly opalescent
Appearance (colour): red
pH-value (25 °C): 7.2 - 7.6
Growth promotion test in accordance with the current version of DIN EN ISO 11133.

Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)):
Inoculum on reference medium (Salmonella enteritidis ATCC 13076 (WDCM 00030)):
Colony count (Salmonella typhimurium ATCC 14028 (WDCM 00031)):
Colony count (Salmonella enteritidis ATCC 13076 (WDCM 00030)):
Recovery on test medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)): ≥ 50 %
Recovery on test medium (Salmonella enteritidis ATCC 13076 (WDCM 00030)): ≥ 50 %
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): Growth or partial inhibition
Growth (Escherichia coli ATCC 8739 (WDCM 00012)): Growth or partial inhibition
Growth (Enterococcus faecalis ATCC 29212 (WDCM 00087)): total inhibition
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)): total inhibition
Colonies (Salmonella typhimurium ATCC 14028 (WDCM 00031)): Colonies with black centre
Colonies (Salmonella enteritidis ATCC 13076 (WDCM 00030)): Colonies with black centre
Colonies (Escherichia coli ATCC 25922 (WDCM 00013)): Yellow colonies
Colonies (Escherichia coli ATCC 8739 (WDCM 00012)): Yellow colonies
Incubation: 24 ± 3 hours at 37 ± 1 °C
A recovery rate of 50 % is equivalent to a productivity value of 0.5.
The indicated colony counts result from the sum of a triple determination.
Reference media: Tryptic Soy Agar

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

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Xylose Lysine Deoxycholate agar GranuCult® prime, according to ISO 6579, ISO 19250, APHA, USDA-FSIS, granular, for Salmonella spp., pkg of 500 g

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1.05287

Xylose Lysine Deoxycholate agar

XLD Agar Heimplate, plate diam. 90 mm, ready-to-use, for long incubation, aseptically filled, box of 20 plates, for Salmonella spp., for Shigella spp.

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XLD Agar

Oxford Agar (Base) GranuCult® prime, according to FDA (BAM), ISO 11290, granular, for Listeria spp., pkg of 500 g

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1.07004

Oxford Agar (Base)

Oxford Agar (Base) GranuCult® prime, according to FDA (BAM), ISO 11290, granular, for Listeria spp., pkg of 500 g

Millipore

1.07004

Oxford Agar (Base)

description

GranuCult® prime, according to ISO 6579, ISO 19250, APHA, USDA-FSIS, granular, for Salmonella spp., pkg of 500 g

description

Heimplate, plate diam. 90 mm, ready-to-use, for long incubation, aseptically filled, box of 20 plates, for Salmonella spp., for Shigella spp.

description

GranuCult® prime, according to FDA (BAM), ISO 11290, granular, for Listeria spp., pkg of 500 g

description

GranuCult® prime, according to FDA (BAM), ISO 11290, granular, for Listeria spp., pkg of 500 g

suitability

Salmonella spp.

suitability

Salmonella spp. (spp.)

suitability

Listeria spp. (spp.)

suitability

Listeria spp. (spp.)

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

application(s)

food and beverages, microbiology

application(s)

microbiology
pharmaceutical

application(s)

food and beverages
microbiology

application(s)

food and beverages
microbiology

agency

APHA, ISO 6579, ISO 19250

agency

EP 2.6.12, USP 61

agency

ISO 11290

agency

ISO 11290

form

granular

form

solid

form

granular

form

granular

manufacturer/tradename

GranuCult® prime

manufacturer/tradename

-

manufacturer/tradename

GranuCult® prime

manufacturer/tradename

GranuCult® prime

Storage Class

11 - Combustible Solids

wgk

WGK 1

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Substances Subject to be Indicated Names

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Substances Subject to be Notified Names

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