1.46073

XLD Agar

Heimplate, plate diam. 90 mm, ready-to-use, for long incubation, aseptically filled, box of 20 plates, for Salmonella spp., for Shigella spp.

• Synonym(s): HEIMPLATE XLD Agar - LI EP+USP 30ml, Xylose Lysine Deoxycholate Agar
• UNSPSC Code: 41106217
• NACRES: NA.74

Properties

• Agency: EP 2.6.12; USP 61
• Quality Level: 300
• sterility: aseptically filled
• form: solid
• feature: ready-to-use for long incubation
• packaging: box of 20 plates
• manufacturer/tradename: Heimplate
• technique(s): microbiological culture: suitable
• plate diam.: 90 mm
• pH: 7.4 ( in H₂O)
• application(s): microbiology; pharmaceutical
• storage temp.: 15-25°C
• suitability: Salmonella spp. (spp.); Shigella spp.

Description

General description

Xylose Lysine Deoxycholate agar (XLD agar) is a selective growth medium introduced by Taylor (1965) for the isolation of Salmonella and Shigella species (Taylor and schelbert, 1971). It relies on the use of selective inhibitory component sodium deoxycholate and three indicator systems, i.e. xylose, lactose and sucrose combined with phenol red. Degradation of xylose, lactose, and sucrose to acid causes phenol red to change its colour to yellow. Sodium thiosulfate and iron (III) salt react to form a precipitate of black iron sulfide in the colonies, indicating the production of hydrogen sulfide. Bacteria which decarboxylate lysine to cadaverine can be recognized by the appearance of a purple coloration around the colonies due to an increase in pH. These reactions can proceed simultaneously or successively. This causes the pH indicator to exhibit various shades of colour, or it may change its colour from yellow to red on prolonged incubation. The culture medium is weakly inhibitory to other microbes due to the presence of sodium deoxycholate.

Application

Xylose Lysine Deoxycholate (XLD) Agar - LI is a selective culture medium for isolation of Salmonella and Shigella in pharmaceuticals.

Packaging

90 mm settle plates with 30 ml filling volume (20 or 120 plates per box) for long incubation (LI)

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 2