03-251
RIPAb+ IGF2BP2 Antibody
clone 5E10-3E1, from mouse
Synonym(s):
Insulin-like growth factor 2 mRNA-binding protein 2, IGF2 mRNA-binding protein 2, IMP-2, Hepatocellular carcinoma autoantigen p62, IGF-II mRNA-binding protein 2, VICKZ family member 2
Select a Size
| Size/SKU | Availability | Price |
|---|---|---|
10 assays | Check Cart for Availability | $663.00 |
About This Item
biological source
mouse
Quality Segment
clone
5E10-3E1, monoclonal
species reactivity
human
manufacturer/tradename
RIPAb+, Upstate®
technique(s)
RIP: suitable, western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
dry ice
Gene Information
human ... IGF2BP2(10644)
General description
IGF2BP2 (Insulin-like growth factor 2 mRNA-binding protein 2) is a member of the IGF-II mRNA-binding protein family that post-transcriptionally regulates IGF2 during developmental stages. IGF2BP1, 2, and 3 bind the UTR region of IGF2 transcripts, and are implicated in the transport of RNA targets, to enable protein synthesis at specific locations in the cytoplasm. Research has shown that the IGF2 gene may be involved in ovarian cancer.
Immunogen
Application
Representative lot data.
HepG2 cell lysate was probed with Anti-IGF2BP2, clone 5E10-3E1 (1:20,000 dilution).
Proteins were visualized using a Goat Anti-Mouse IgG secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates IGF2BP2 (~66 kDa).
Note: An uncharacterized band appears at ~50 kDa in some lysates.
Packaging
Physical form
Concentration: 0.176 mg/mL
Normal mouse IgG. One vial containing 125 µg purified mouse IgG in 125 µL storage buffer containing 0.1% sodium azide. Store at -20°C.
RIP Primer IGF2. One vial containing 75 μL of 5 μM of each primer specific for human IGF2 mRNA. Store at -20°C.
FOR: GCG GCT TCT ACT TCA GCA G
REV: CAG GTG TCA TAT TGG AAG AAC
Analysis Note
Representative lot data
RIP Lysate prepared from 293 cells (2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using 1.25 µg of either a normal mouse IgG or 1.25 µg Anti-IGF2BP2 antibody and the Magna RIP® RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700).
Successful immunoprecipitation of IGF2BP2-associated RNA was verified by qPCR using RIP Primers IGF2, (Figure 1).
Please refer to the Magna RIP
Other Notes
Legal Information
1 of 1
This Item | |||
|---|---|---|---|
| description clone 5E10-3E1, from mouse | description from rabbit, purified by affinity chromatography | description from rabbit, purified by affinity chromatography | description clone 2A8, from mouse |
| technique(s) RIP: suitable, western blot: suitable | technique(s) RIP: suitable, immunoprecipitation (IP): suitable, western blot: suitable | technique(s) RIP: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable | technique(s) RIP: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
| biological source mouse | biological source rabbit | biological source rabbit | biological source mouse |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| manufacturer/tradename RIPAb+, Upstate® | manufacturer/tradename RIPAb+, Upstate® | manufacturer/tradename RIPAb+, Upstate® | manufacturer/tradename RIPAb+, Upstate® |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
Storage Class
10-13 - German Storage Class 10 to 13
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