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Anti-trimethyl-Histone H3 (Lys4) Antibody

Name: Anti-trimethyl-Histone H3 (Lys4) Antibody
Brand: MM
Price: 707 USD
Availability: InStock

Upstate^®, from rabbit

Synonym(s):

H3K4me3, Histone H3 (tri methyl K4), H3 histone family, member T, histone 3, H3, histone cluster 3, H3

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Size/SKU	Availability	Price
100 μL	Check Cart for Availability	$707.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Clone:

polyclonal

Species reactivity:

human

Application:

ChIP, DB, ICC, WB

Citations:

1097

$707.00

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Properties

biological source

rabbit

Quality Segment

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

mol wt

(~17 kDa observed; 15.51 kDa calculated.. Uncharacterized bands may be observed in some lysate(s).)

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

vertebrates (based on 100% sequence homology.)

manufacturer/tradename

Upstate^®

technique(s)

ChIP: suitable (ChIP-seq), dot blot: suitable, immunocytochemistry: suitable, western blot: suitable

NCBI accession no.

NM_003493

UniProt accession no.

Q16695

shipped in

wet ice

target post-translational modification

trimethylation (Lys4)

Gene Information

human ... H3C1(8350)

Description

General description

Anti-trimethyl-Histone H3 (Lys4), Cat. No. 07-473, is a rabbit polyclonal antibody that detects Histone H3 trimethylated on lysine 4 and is tested for use in ChIP, Dot Blot, Immunocytochemistry, and Western Blotting.

Histone H3.1t (UniProt: Q16695; also known as H3/t, H3t, H3/g) is encoded by the HIST3H3 (also known as H3FT) gene (Gene ID: 8290) in human. Histones are highly conserved basic nuclear proteins that are responsible for the nucleosome structure of chromatin in eukaryotes. They play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which DNA is wrapped in repeating units, called nucleosomes, which limits DNA accessibility to the cellular machineries that require DNA as a template. Histone H3 features a main globular domain and a long N-terminal tail, which protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. The N-terminal tails of histone proteins are subject to posttranslational modifications, including phosphorylation, acetylation, and methylation, which recruit downstream regulatory factors, influence chromatin structure, and are critical determinants of transcription. Histone H3 can undergo acetylation on several lysine residues in the histone tail by histone acetyltransferases. Acetylation is generally associated with transcriptional activity and methylation of lysine and arginine residues can either activate or repress depending on the residue modified. Trimethylation of histone H3 is one of the most studied epigenetic marks. H3K4me3 modifications are reported to occur consistently at transcription start sites and H3K4me3 domain is associated with higher transcription activity and cell identity in pre-implantation development and in the process of deriving embryonic stem cells from the inner cell mass and trophoblast stem cells from the trophectoderm. (Ref.: Sharifi-Zarchi, A., et al. (2017). BMS Genomics. 18; Article 964; Liu, X., et al. (2016). Nature. 537(7621); 558-562).

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide

Immunogen

Epitope: Trimethylated (Lys4)

KLH-conjugated linear peptide corresponding to 10 amino acids from the N-terminal region of human Histone H3 trimethylated on lysine 4.

Application

ChIP Analysis:
An independent lab has shown that this antibody performs in chromatin immunoprecipitation (ChIP).
Dot Blot Analysis: A 1:10,000 dilution from a representative lot detected trimethyl-Histone H3 (Lys 4 ) in an Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and an Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).
ChIP-seq Analysis:
Chromatin immunoprecipitation was performed using the Magna ChIP HiSens kit (cat# 17-10460), 1 µL Anti-trimethyl-Histone H3 (Lys4) antibody (cat# 07-473) , 20 µL Protein A/G beads, and 1e6 crosslinked HeLa cell chromatin followed by DNA purification using magnetic beads. Libraries were prepared from Input and ChIP DNA samples using standard protocols with Illumina barcoded adapters, and analyzed on Illumina HiSeq instrument. An excess of eighteen million reads from FastQ files were mapped using Bowtie (http://bowtie-bio.sourceforge.net/manual.shtml) following TagDust (http://genome.gsc.riken.jp/osc/english/dataresource/) tag removal. Peaks were identified using MACS (http://luelab.dfci.harvard.edu/MACS/), with peaks and reads visualized as a custom track in UCSC Genome Browser (http://genome.ucsc.edu) from BigWig and BED files. The highest 25% of peaks identified in the 07-473 dataset showed 99% overlap with peaks identified in the ENCODE H3K4me3 BROAD Histone track for HeLa S3.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Biochem/physiol Actions

This rabbit polyclonal antibody specifically detects Histone H3 trimethylated on lysine 4 (H3Kme3).

Physical form

Affinity purified

Affinity purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8ºC from date of receipt.

Analysis Note

Control
HeLa nuclear extracts

Evaluated by Western Blotting on HeLa acid extracted nuclear preps (positive) and recombinant Histone H3 (negative).

Western Blotting Analysis:
1:5,000 – 1:10,000 dilution of this antibody was used to detect trimethylated-Histone H3 (Lys4) in HeLa nuclear acid extracts.

Other Notes

Replaces: 04-791

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	07436	07030	07442
Sigma-Aldrich 07473 Anti-trimethyl-Histone H3 (Lys4) Antibody	Sigma-Aldrich 07436 Anti-monomethyl-Histone H3 (Lys4) Antibody	Sigma-Aldrich 07030 Anti-dimethyl-Histone H3 (Lys4) Antibody	Sigma-Aldrich 07442 Anti-trimethyl-Histone H3 (Lys9) Antibody
description Upstate^®, from rabbit	description Upstate^®, from rabbit	description Upstate^®, from rabbit	description Upstate^®, from rabbit
antibody form affinity isolated antibody	antibody form purified immunoglobulin	antibody form unpurified	antibody form purified antibody
species reactivity human	species reactivity human, vertebrates	species reactivity Tetrahymena sp., human	species reactivity human, mouse, rat, chicken
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
clone polyclonal	clone polyclonal	clone polyclonal	clone polyclonal
technique(s) ChIP: suitable (ChIP-seq), immunocytochemistry: suitable, dot blot: suitable, western blot: suitable	technique(s) ChIP: suitable (ChIP-seq), dot blot: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, inhibition assay: suitable (peptide), western blot: suitable	technique(s) ChIP: suitable (ChIP-seq), dot blot: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, inhibition assay: suitable (peptide), western blot: suitable	technique(s) ChIP: suitable (ChIP-seq), dot blot: suitable, immunocytochemistry: suitable, inhibition assay: suitable (peptide), multiplexing: suitable, western blot: suitable
mol wt (~17 kDa observed; 15.51 kDa calculated.. Uncharacterized bands may be observed in some lysate(s).)	mol wt -	mol wt -	mol wt -

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