17-655
ChIPAb+ Nanog Antibody - ChIP Validated Antibody and Primer Set
from mouse
Synonym(s):
homeobox protein NANOG, homeobox transcription factor Nanog, hNanog
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| Size/SKU | Availability | Price |
|---|---|---|
25 assays | Estimated to ship onAugust 27, 2026 | $634.00 |
About This Item
UNSPSC Code:
12352203
NACRES:
NA.75
eCl@ss:
32160702
Clone:
monoclonal
Species reactivity:
human
Application:
ChIP, ICC, WB
Citations:
-
biological source
mouse
Quality Segment
antibody form
purified immunoglobulin
clone
monoclonal
species reactivity
human
manufacturer/tradename
ChIPAb+, Upstate®
technique(s)
ChIP: suitable, immunocytochemistry: suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
General description
All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ Nanog set includes a Nanog antibody, a Normal Mouse IgG, and positive control primers which amplify a 81 bp of human miR-302 cluster promoter region. The Nanog antibody and negative control IgG are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of Nanog associated chromatin
The ChIPAb+ Nanog set includes a Nanog antibody, a Normal Mouse IgG, and positive control primers which amplify a 81 bp of human miR-302 cluster promoter region. The Nanog antibody and negative control IgG are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of Nanog associated chromatin
Application
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from Ntera-2 cells fixed for 15 min (1e5 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 3 µg of either Normal Mouse IgG (Part No. CS200621), or 3 µg Anti-Nanog (Part No. CS207343) and the Magna ChIP
Please refer to the Magna ChIP
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from Ntera-2 cells fixed for 15 min (1e4, 1e5 and 1e6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 3 µg of either Normal Mouse IgG (Part No. CS200621), or 3 µg Anti-Nanog (Part No. CS207343) and the Magna ChIP
Please refer to the MagnaChIP
Western Blotting Analysis:
0.5 µg/mL of this antibody detected NANOG on 10 µg of H9 human embryonic stem cell lysate.
Immunocytochemistry Analysis:
A 1:500 dilution from a representative lot detected NANOG in H9 human embryonic stem cells.
Sonicated chromatin prepared from Ntera-2 cells fixed for 15 min (1e5 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 3 µg of either Normal Mouse IgG (Part No. CS200621), or 3 µg Anti-Nanog (Part No. CS207343) and the Magna ChIP
Please refer to the Magna ChIP
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from Ntera-2 cells fixed for 15 min (1e4, 1e5 and 1e6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 3 µg of either Normal Mouse IgG (Part No. CS200621), or 3 µg Anti-Nanog (Part No. CS207343) and the Magna ChIP
Please refer to the MagnaChIP
Western Blotting Analysis:
0.5 µg/mL of this antibody detected NANOG on 10 µg of H9 human embryonic stem cell lysate.
Immunocytochemistry Analysis:
A 1:500 dilution from a representative lot detected NANOG in H9 human embryonic stem cells.
Research Category
Epigenetics & Nuclear Function
Nuclear Function
Epigenetics & Nuclear Function
Nuclear Function
Use ChIPAb+ Nanog ChIP Validated mouse monoclonal antibody for the detection of Nanog.
Packaging
25 assays per set. Recommended use: 3.0 μg of antibody per chromatin immunoprecipitation (dependent upon biological context).
Physical form
Anti-Nanog (Mouse Monoclonal), Part No. CS207343. One vial containing 110 µL (0.7 mg/mL) purified mouse monoclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4),150mM NaCl, and 0.05% sodium azide, before the addition of 30% glycerol. Store at -20°C.
Normal Mouse IgG, Part No. CS200621. One vial containing 125 μg of purified mouse IgG in 125 μL storage buffer containing 0.1% sodium azide. Store at -20°C.
ChIP Primers, miR-302. Part No. CS207380. One vial containing 75 μL of 5 μM of each primer specific for human miR-302 cluster promoter region (chr4:113570181-113570261, hg19 build). Store at -20°C.
FOR: CCC GTG GAA GCA ATC TAT TTA TTT
REV: TGT GTT TCT ATC TGG AGG AAC TCT GT
Normal Mouse IgG, Part No. CS200621. One vial containing 125 μg of purified mouse IgG in 125 μL storage buffer containing 0.1% sodium azide. Store at -20°C.
ChIP Primers, miR-302. Part No. CS207380. One vial containing 75 μL of 5 μM of each primer specific for human miR-302 cluster promoter region (chr4:113570181-113570261, hg19 build). Store at -20°C.
FOR: CCC GTG GAA GCA ATC TAT TTA TTT
REV: TGT GTT TCT ATC TGG AGG AAC TCT GT
Format: Purified
Analysis Note
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from Ntera-2 cells fixed for 15 min (1e5 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 3 µg of either Normal Mouse IgG (Part No. CS200621), or 3 µg Anti-Nanog (Part No. CS207343) and the Magna ChIP® HiSens Kit (Cat. # 17-10460). Successful immunoprecipitation of Nanog associated DNA fragments was verified by qPCR using ChIP Primers, miR-302 (Part No. CS207380).
Please refer to the MagnaChIP
Sonicated chromatin prepared from Ntera-2 cells fixed for 15 min (1e5 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 3 µg of either Normal Mouse IgG (Part No. CS200621), or 3 µg Anti-Nanog (Part No. CS207343) and the Magna ChIP® HiSens Kit (Cat. # 17-10460). Successful immunoprecipitation of Nanog associated DNA fragments was verified by qPCR using ChIP Primers, miR-302 (Part No. CS207380).
Please refer to the MagnaChIP
Control
Includes normal mouse IgG and primers specific for human miR-302 cluster promoter region.
Includes normal mouse IgG and primers specific for human miR-302 cluster promoter region.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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This Item | |||
|---|---|---|---|
| description from mouse | description from mouse | description from rabbit | description from mouse |
| antibody form purified immunoglobulin | antibody form - | antibody form - | antibody form - |
| clone monoclonal | clone monoclonal | clone polyclonal | clone monoclonal |
| biological source mouse | biological source mouse | biological source rabbit | biological source mouse |
| species reactivity human | species reactivity human, Xenopus | species reactivity human | species reactivity human |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| technique(s) ChIP: suitable, western blot: suitable, immunocytochemistry: suitable | technique(s) ChIP: suitable (ChIP-seq), immunoprecipitation (IP): suitable | technique(s) ChIP: suitable, western blot: suitable | technique(s) ChIP: suitable, immunocytochemistry: suitable, western blot: suitable |
Storage Class
10 - Combustible liquids
wgk
WGK 1
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