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PI 3-Kinase 3-step HTRF^® Assay

Name: PI 3-Kinase 3-step HTRF<SUP>®</SUP> Assay
Brand: MM
Price: 21130 USD
Availability: InStock

The PI 3-Kinase 3-Step HTRF Assay is a high-performance assay kit that provides a universal method for testing all Class I PI3-Kinases in a homogeneous format.

Synonym(s):

3-Step HTRF Assay, PI 3-Kinase Assay

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Size/SKU	Availability	Price
10000 wells	Check Cart for Availability	$21,130.00

About This Item

UNSPSC Code:

12161503

NACRES:

NA.84

eCl@ss:

32161000

$21,130.00

Check Cart for Availability

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Properties

Quality Segment

100

species reactivity

human, mouse

manufacturer/tradename

HTRF^®, Upstate^®

technique(s)

activity assay: suitable (kinase)

NCBI accession no.

NM_006218.2

UniProt accession no.

P42336

detection method

fluorometric (HTRF)

shipped in

dry ice

Gene Information

human ... PIK3CA(5290)

Description

General description

Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit α (PIK3CA) gene is mapped to human chromosome 3q26.32. This gene codes for the catalytic subunit p110α of class IA PI3-kinase. PI3K signaling pathways regulate various cellular activities and properties including proliferation, survival, apoptosis, motility, and morphology. Mutation or overexpression of the PIK3CA gene is implicated in the development of various human solid tumors including head and neck cancers or pharyngeal cancer and ovarian cancer.

Application

PI 3-Kinase 3-step HTRF^® Assay has been used to determine the in vitro inhibitory potencies of phosphatidylinositol 3-kinase (PI3K) δ inhibitors such as MSD-496486311, MSD126796721, idelalisib, and duvelisib across PI3K α, β, γ, and δ isoforms.

Biochem/physiol Actions

Target Sub-Family: PI3K/PI4K

Packaging

Kit capacity: 10,000 wells

Preparation Note

12 months at -70°C

Other Notes

4X Reaction Buffer

PIP2 (substrate, 1mM stock)

DM A Reaction Buffer

DM B Reaction Buffer

DM C Reaction Buffer

BPIP3

Stop Reagent

Research Category
Apoptosis & Cancer
Research Sub Category
Oncology

Legal Information

HTRF is a registered trademark of Cis Bio International

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	3304A	33036	14782M
Sigma-Aldrich 33047 PI 3-Kinase 3-step HTRF^® Assay	Sigma-Aldrich 3304A PI 3-Kinase 3-step HTRF^® Assay	Sigma-Aldrich 33036 PI 3-Kinase HTRF^® Assay; 10,000 wells	Sigma-Aldrich 14782M PI 3-Kinase (p110α(E542K)/p85α), Human, 10 µg
description The PI 3-Kinase 3-Step HTRF Assay is a high-performance assay kit that provides a universal method for testing all Class I PI3-Kinases in a homogeneous format.	description Everything you need to perform the PI3 Kinase 3-step HTRF assays (1920 wells).	description The PI 3-Kinase HTRF Assay is a high-performance assay kit that provides a universal method for testing all Class I PI3-Kinases in a homogeneous format. This pack size has a screening capacity of 10,000 wells.	description Complex of N-terminal 6His-tagged, recombinant, full-length human p110α containing the mutation E542K & untagged, recombinant, full-length human p85α. For use in Kinase Assays.
technique(s) activity assay: suitable (kinase)	technique(s) activity assay: suitable (kinase)	technique(s) activity assay: suitable (kinase)	technique(s) activity assay: suitable (kinase)
manufacturer/tradename HTRF^®, Upstate^®	manufacturer/tradename HTRF^®, Upstate^®	manufacturer/tradename HTRF^®, Upstate^®	manufacturer/tradename Upstate^®
shipped in dry ice	shipped in -	shipped in dry ice	shipped in -
Quality Level 100	Quality Level 100	Quality Level 100	Quality Level 100, 300
NCBI accession no. NM_006218.2	NCBI accession no. NM_006218.2	NCBI accession no. NM_006218.2	NCBI accession no. NM_006218.2
UniProt accession no. P42336	UniProt accession no. P42336	UniProt accession no. P42336	UniProt accession no. P42336

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pictograms

GHS07

signalword

Warning

hcodes

H319

pcodes

P264 - P280 - P305 + P351 + P338 - P337 + P313

Hazard Classifications

Eye Irrit. 2

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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