MABC1638
Anti-AIFM2 Antibody, clone 6D8-11
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About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
6D8, monoclonal
Application:
ELISA, ICC, IF, WB
Citations:
-
Pricing and availability is not currently available.
biological source
rat
Quality Segment
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
6D8, monoclonal
mol wt
calculated mol wt 40.53 kDa, observed mol wt ~40 kDa
purified by
using protein G
species reactivity
human
packaging
antibody small pack of 100 μL
technique(s)
ELISA: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, western blot: suitable
isotype
IgG2a
epitope sequence
Unknown
Protein ID accession no.
UniProt accession no.
shipped in
2-8°C
target post-translational modification
unmodified
Gene Information
rat ... AIFM2(84883)
General description
Ferroptosis suppressor protein 1 (UniProt: Q9BRQ8; also known as FSP1, Apoptosis-inducing factor homologous mitochondrion-associated inducer of death, AMID, p53-responsive gene 3 protein, AIFM2) is encoded by the AIFM2 (also known as AMID, PRG3) gene (Gene ID: 84883) in human. AIFM2 is a lipid-anchor membrane protein of the FAD-dependent oxidoreductase family that is also detected in the nucleus and mitochondrial membrane. It is highly expressed in heart and moderately in liver and skeletal muscle. Low expression levels are observed in placenta, lung, kidney, and pancreas. It is reported to be down-regulated in a number of human tumors. It serves as a NAD(P)H-dependent oxidoreductase and is involved in cellular oxidative stress response. At the plasma membrane, it catalyzes reduction of coenzyme Q/ubiquinone-10 to ubiquinol-10, a lipophilic radical-trapping antioxidant that prevents lipid oxidative damage and consequently ferroptosis. It is shown to cooperates with GPX4 to suppress phospholipid peroxidation and ferroptosis and its anti-ferroptotic function is reported to be independent of cellular glutathione levels. It also plays a role in mitochondrial stress signaling. Upon induction of oxidative stress, it associates with the lipid peroxidation end product 4-hydroxy-2-nonenal (HNE) forming a lipid adduct. This HNE adduct inactivates the NADH oxidoreductase activity of AIFM2 and facilitates its translocation from mitochondria into the nucleus triggering DNA damage and cell death. (Ref.: Doll, S., et al. (2019). Nature 575(7784); 693-698; Miriyala, S., et al. (2016). Free Radic. Biol. Med. 91; 68-80).
Immunogen
SUMO-His-tagged full-length recombinant human Ferroptosis suppressor protein 1 (FSP1/AIFM2).
Application
Quality Control Testing
Evaluated by Western Blotting in A549 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected AIFM2 in A549 cell lysate.
Tested Appliacations
Western Blotting Analysis: A 1:500 dilution from a representative lot detected AIFM2 in HepG2 cell lysate.
Immunocytochemistry Analysis: A 1:25 Dilution from a representative lot detected AIFM2 in HepG2 cells.
Immunofluorescence Analysis: A representative lot detected AIFM2 in Immunofluorescence applications (Doll, S. et al. (2019). Nature.;575(7784):693-698).
Western Blotting Analysis: A representative lot detected AIFM2 in Western Blotting applications (Doll, S. et al. (2019). Nature.;575(7784):693-698).
ELISA Analysis: A representative lot detected AIFM2 in ELISA applications (Doll, S. et al. (2019). Nature.;575(7784):693-698).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in A549 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected AIFM2 in A549 cell lysate.
Tested Appliacations
Western Blotting Analysis: A 1:500 dilution from a representative lot detected AIFM2 in HepG2 cell lysate.
Immunocytochemistry Analysis: A 1:25 Dilution from a representative lot detected AIFM2 in HepG2 cells.
Immunofluorescence Analysis: A representative lot detected AIFM2 in Immunofluorescence applications (Doll, S. et al. (2019). Nature.;575(7784):693-698).
Western Blotting Analysis: A representative lot detected AIFM2 in Western Blotting applications (Doll, S. et al. (2019). Nature.;575(7784):693-698).
ELISA Analysis: A representative lot detected AIFM2 in ELISA applications (Doll, S. et al. (2019). Nature.;575(7784):693-698).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-AIFM2, clone 6D8-11, Cat. No. MABC1638, is a rat monoclonal antibody that detects Ferroptosis suppressor protein 1 and is tested for use in ELISA, Immunocytochemistry, Immunofluorescence, and Western Blotting.
Biochem/physiol Actions
Clone 6D8 is a rat monoclonal antibody that detects human Ferroptosis suppressor protein 1 (AIFM2).
Physical form
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
|---|---|---|---|
| description - | description recombinant, expressed in HEK 293 cells | description Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody | description affinity isolated antibody |
| antibody form purified antibody | antibody form purified antibody | antibody form affinity isolated antibody | antibody form affinity isolated antibody |
| biological source rat | biological source rabbit | biological source rabbit | biological source rabbit |
| species reactivity human | species reactivity mouse, human | species reactivity human | species reactivity human |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| clone 6D8, monoclonal | clone 1I15, recombinant monoclonal | clone polyclonal | clone polyclonal |
| Gene Information rat ... AIFM2(84883) | Gene Information - | Gene Information human ... AIFM2(84883) | Gene Information human ... AIFM2(84883) |
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Storage Class
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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