The only difference between S7110 and S7111 is that S7111 includes the ApopTag Positive Control Slides.
S7110
ApopTag Fluorescein In Situ Apoptosis Detection Kit
The ApopTag Fluorescein In Situ Apoptosis Detection Kit detects apoptotic cells in situ by the indirect TUNEL method, utilizing an anti-digoxigenin antibody that is conjugated to a Fluorescein reporter molecule.
Synonym(s):
Apoptosis detection kit
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| Size/SKU | Availability | Price |
|---|---|---|
40 assays | Estimated to ship TODAY | $869.00 |
About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
eCl@ss:
32161000
Technique(s):
flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
Detection method:
fluorometric
Shipped in:
dry ice
Quality Segment
manufacturer/tradename
ApopTag, Chemicon®
technique(s)
flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
detection method
fluorometric
shipped in
dry ice
General description
The ApopTag
The ApopTag
The ApopTag
Application
INTRODUCTION
ApopTag
Principles of the Procedure
The reagents provided in all ApopTag
DNA fragments which have been labeled with the digoxigenin-nucleotide are then allowed to bind an anti-digoxigenin antibody that is conjugated to fluorescein (Figure 1A). Fluorescent antibodies provide sensitive detection in immunohistochemistry or immunocytochemistry (i.e. on tissue or cells) and are not subject to experimental variations due to the substrate or the development step. This mixed molecular biological-histochemical systems allows for sensitive and specific staining of very high concentrations of 3′-OH ends that are localized in apoptotic bodies.The ApopTag
ApopTag
Principles of the Procedure
The reagents provided in all ApopTag
DNA fragments which have been labeled with the digoxigenin-nucleotide are then allowed to bind an anti-digoxigenin antibody that is conjugated to fluorescein (Figure 1A). Fluorescent antibodies provide sensitive detection in immunohistochemistry or immunocytochemistry (i.e. on tissue or cells) and are not subject to experimental variations due to the substrate or the development step. This mixed molecular biological-histochemical systems allows for sensitive and specific staining of very high concentrations of 3′-OH ends that are localized in apoptotic bodies.The ApopTag
The ApopTag Fluorescein In Situ Apoptosis Detection Kit detects apoptotic cells in situ by the indirect TUNEL method, utilizing an anti-digoxigenin antibody that is conjugated to a Fluorescein reporter molecule.
Preparation Note
1. Store the kit at -15°C to -25°C until the first use. After the first use, if the kit will be used within three months, store the TdT Enzyme (#90418) at -15°C to -25°C and store the remaining components at 2°C to 8°C.
2. Protect the anti-digoxigenin fluorescein antibody (#90426) from unnecessary exposure to light.
Precautions
1. The following kit components contain potassium cacodylate (dimethylarsinic acid) as a buffer: Equilibration Buffer (#90416), Reaction Buffer (#90417), and TdT Enzyme (#90418). These components are harmful if swallowed; avoid contact with skin and eyes (wear gloves, glasses) and wash areas of contact immediately.
2. Antibody Conjugates (#90426) and Blocking Solutions (#90425) contain 0.08% sodium azide as a preservative.
3. TdT Enzyme (#90418) contains glycerol and will not freeze at -20°C. For maximum shelf life, do not warm this reagent to room temperature before dispensing.
2. Protect the anti-digoxigenin fluorescein antibody (#90426) from unnecessary exposure to light.
Precautions
1. The following kit components contain potassium cacodylate (dimethylarsinic acid) as a buffer: Equilibration Buffer (#90416), Reaction Buffer (#90417), and TdT Enzyme (#90418). These components are harmful if swallowed; avoid contact with skin and eyes (wear gloves, glasses) and wash areas of contact immediately.
2. Antibody Conjugates (#90426) and Blocking Solutions (#90425) contain 0.08% sodium azide as a preservative.
3. TdT Enzyme (#90418) contains glycerol and will not freeze at -20°C. For maximum shelf life, do not warm this reagent to room temperature before dispensing.
Other Notes
Equilibration Buffer 90416 3.0 mL -15°C to -25°C
Reaction Buffer 90417 2.0 mL -15°C to -25°C
TdT Enzyme 90418 0.64 mL -15°C to -25°C
Stop/Wash Buffer 90419 20 mL -15°C to -25°C
Blocking Solution 90425 2.6 mL -15°C to -25°C
Anti-Digoxigenin-Fluorescein* 90426 2.1 mL 2°C to 8°C
Plastic Coverslips 90421 100 ea. Room Temp.
*affinity purified sheep polyclonal antibody
Reaction Buffer 90417 2.0 mL -15°C to -25°C
TdT Enzyme 90418 0.64 mL -15°C to -25°C
Stop/Wash Buffer 90419 20 mL -15°C to -25°C
Blocking Solution 90425 2.6 mL -15°C to -25°C
Anti-Digoxigenin-Fluorescein* 90426 2.1 mL 2°C to 8°C
Plastic Coverslips 90421 100 ea. Room Temp.
*affinity purified sheep polyclonal antibody
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| description The ApopTag Fluorescein In Situ Apoptosis Detection Kit detects apoptotic cells in situ by the indirect TUNEL method, utilizing an anti-digoxigenin antibody that is conjugated to a Fluorescein reporter molecule. | description The ApopTag Plus Fluorescein In Situ Apoptosis Detection Kit detects apoptotic cells in situ by the indirect TUNEL method, utilizing an anti-digoxigenin antibody that is conjugated to a fluorescein reporter molecule. | description The CaspaTag Caspase-8 In Situ Assay Kit, Fluorescein for flow cytometry is a fluorescent-based assay for detection of active caspase-8 in cells undergoing apoptosis. | description The In Situ FLICA Pan-Caspase Detection Kit for flow cytometry is a fluorescent-based assay for detection of active caspases in cells undergoing apoptosis. |
| technique(s) flow cytometry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunocytochemistry: suitable | technique(s) flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | technique(s) activity assay: suitable, flow cytometry: suitable | technique(s) activity assay: suitable, flow cytometry: suitable |
| manufacturer/tradename ApopTag, Chemicon® | manufacturer/tradename ApopTag, Chemicon® | manufacturer/tradename CaspaTag, Chemicon® | manufacturer/tradename CaspaTag, Chemicon® |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| shipped in dry ice | shipped in dry ice | shipped in wet ice | shipped in wet ice |
| detection method fluorometric | detection method fluorometric | detection method fluorometric | detection method fluorometric |
signalword
Danger
target_organs
Respiratory Tract
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
hcodes
Hazard Classifications
Aquatic Chronic 2 - Carc. 1B - ED ENV 1 - STOT RE 2 Inhalation
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