This product is soluble at 1 mM in water. The Certificate of Analysis recommends using sterile distilled water. Tissue culture grade water is suitable.
SCM075
ROCK Inhibitor (Y-27632)
The ROCK Inhibitor (Y-27632) is available in a 5 mg format & has been optimized & validated for cell culture & Neuroscience applications.
Synonym(s):
Y-27632
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| Size/SKU | Availability | Price |
|---|---|---|
5 mg | Please contact Customer Service for Availability | $1,210.00 |
About This Item
UNSPSC Code:
12352207
NACRES:
NA.75
eCl@ss:
32160801
$1,210.00
Please contact Customer Service for Availability
Quality Segment
form
lyophilized
mol wt
320.26 g/mol
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
-10 to -25°C
General description
ROCK Inhibitor (Y-27632) is a selective, ATP-competitive inhibitor of Rho-associated protein kinase (ROCK) including p160Rock (Ki=140nM) and Rock-II. Also acts as a potent inhibitor of agonist-induced Ca2+ sensitization of myosin phosphorylation and smooth muscle contraction. One-hour treatment with 10uM Y-27632 blocks apoptosis of dissociated cultured human embryonic stem cells (hESCs), increases survival and cloning efficiency of hESCs without affecting their pluripotency1. Recently, some reports showed that the application of Y-27632 and Accutase (Cat. No. SCR005), a formulated mixture of digestive enzymes, enhances the post-thaw survival rate of single hESCs using conventional slow freezing and rapid thawing methods
Preparation Note
Before Reconstitution: Maintain at -20ºC, protected from light for 2 years. After Reconstitution: 1 year at -20ºC, protected from light
1 of 1
This Item | |||
|---|---|---|---|
| description The ROCK Inhibitor (Y-27632) is available in a 5 mg format & has been optimized & validated for cell culture & Neuroscience applications. | description Y-27632A, CAS 331752-47-7, is a cell-permeable, reversible, inhibitor of Rho kinases (Ki = 140 nM for p160ROCK). Enhances survival & cloning efficiency of ESC without affecting their pluripotency. | description InSolution, ≥95%, reversible, inhibitor of Rho kinases | description A cell-permeable, pyrrolidine small molecule that promotes single human embryonic stem cell survival up to 20-fold over background with equal or higher potency than Y-27632. |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| form lyophilized | form solid | form liquid | form syrup |
| storage temp. -10 to -25°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in dry ice | shipped in ambient | shipped in wet ice | shipped in wet ice |
| mol wt 320.26 g/mol | mol wt - | mol wt - | mol wt - |
| technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) - | technique(s) - |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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