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SCM303

Hindgut Induction Medium

Ready-to-use serum-free media used for the efficient differentiation of iPSC derived definitive endoderm (DE) cells to CDX-2+ hindgut endoderm cells.

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Size/SKUAvailabilityPrice
50 mL
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$501.00

About This Item

UNSPSC Code:
41106514
NACRES:
NA.71

$501.00

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form

liquid

packaging

pkg of 50 mL

technique(s)

cell culture | stem cell: suitable

impurities

<2 EU/mL endotoxin

pH

7.0-7.4

General description

The hindgut is the region of the gut tube lying at the distal third of the transverse colon and gives rise to splenic flexure, descending colon, sigmoid colon and rectum. Additionally, the endodermal layer of the hindgut region forms the epithelial lining of the urinary bladder and urethra. The main function of the hindgut is to absorb most of the water and nutrients remaining from partially digested food. The Hindgut Induction Medium is a defined ready-to-use serum-free media used for the efficient differentiation of definitive endoderm cells to CDX-2 positive hindgut endoderm cells. The 4-day differentiation protocol is simple and has been validated on two human iPS cell lines. Differentiated cells express high levels of the hindgut endoderm marker, CDX-2. CDX-2 hindgut endoderm cells can be further differentiated into specialized lineages such as intestinal colon organoids after embedding in Matrigel and cultured in 3dGRO® Human Colon Organoid Expansion Medium (SCM304).

Application

Ready-to-use serum-free media used for the efficient differentiation of iPSC derived definitive endoderm (DE) cells to CDX-2+ hindgut endoderm cells.

Research Category:
  • Stem Cell Research
  • Cell Culture

Preparation Note

Upon receipt, store at -20°C. When ready to use, thaw overnight at 2-8°C. Once thawed, mix thoroughly and aliquot into smaller volumes (i.e. 10 mL), use immediately and store at 2-8°C for up to 1 week. Do not re-freeze. Unused aliquots may be stored at -20°C until the expiry date.

Analysis Note

Appearance: Clear liquid/no particulates
Sterility Tested: No growth
Endotoxin: <2 EU/mL
pH: 7.0−7.4
Mycoplasma: Negative
Functional Assay: >60% CDX-2+ expression after 4 days of differentiation from iPSC derived DE cells.

Legal Information

3dGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item
SCM012SCM105NIST3671
Hindgut Induction Medium Ready-to-use serum-free media used for the efficient differentiation of iPSC derived definitive endoderm (DE) cells to CDX-2+ hindgut endoderm cells.

SCM303

Hindgut Induction Medium

NDiff Neuro-2 Medium Supplement (200x) NDiff Neuro-2 Medium Supplement is a serum-free, N2-like supplement for the in vitro differentiation of murine embryonic stem (ES) cells into post-mitotic neurons particularly via monolayer differentiation.

Sigma-Aldrich

SCM012

NDiff Neuro-2 Medium Supplement (200x)

3dGRO&#174; L-WRN Conditioned Media Supplement

Sigma-Aldrich

SCM105

3dGRO® L-WRN Conditioned Media Supplement

vibrant-m

NIST3671

Nicotine metabolites in human urine (frozen)

description

Ready-to-use serum-free media used for the efficient differentiation of iPSC derived definitive endoderm (DE) cells to CDX-2+ hindgut endoderm cells.

description

NDiff Neuro-2 Medium Supplement is a serum-free, N2-like supplement for the in vitro differentiation of murine embryonic stem (ES) cells into post-mitotic neurons particularly via monolayer differentiation.

description

-

description

NIST® SRM® 3671

form

liquid

form

liquid

form

liquid

form

-

technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | stem cell: suitable

technique(s)

cell based assay: suitable

technique(s)

LC/MS: suitable, GC/MS: suitable

packaging

pkg of 50 mL

packaging

-

packaging

-

packaging

pkg of 3 x 10 mL

impurities

<2 EU/mL endotoxin

impurities

-

impurities

-

impurities

-

pH

7.0-7.4

pH

-

pH

-

pH

-

Storage Class

12 - Non Combustible Liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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