Yes. When performing fluorescent staining procedures, aldehydes and alcohols are common fixatives. Paraformaldehyde solutions are prepared without the use of methanol. It is believed that methanol can adversely affect the staining of membrane bound proteins. It it for this reason that freshly prepared paraformaldehyde solutions are preferred over formaldehyde solutions that may contain up to 10 or 15% methanol to keep the formaldehyde from re-polymerizing back into solid paraformaldehyde. When using paraformaldehyde to fix tissue, frozen sections are preferred over paraffin processing.
42874
Tetramethylrhodamine isothiocyanate–Dextran
average mol wt 40,000
Synonym(s):
TRITC-Dextran 40, TRITC-Dextran
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 g | Available to ship TODAYfromMILWAUKEE | $707.00 |
About This Item
$707.00
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biological source
synthetic
Quality Level
form
powder
mol wt
average mol wt 40,000
extent of labeling
0.001-0.008 mol/mol TRITC glucose
fluorescence
λex 550 nm; λem 577 nm±5 nm in 0.1 M Tris pH 8.0
application(s)
cell analysis
General description
Tetramethylrhodamine isothiocyanate–Dextran, also known as TRITC-Dextran or TRITC-Dextran 40, is a fluorescent dye and a novel drug. The fluorescent activity of TRITC-Dextran is detected at 555nm wavelength.[1] The TRITC-Dextran molecules are released in the cells through passive diffusion due to a concentration gradient.[2]
Application
Tetramethylrhodamine isothiocyanate–Dextran (TRITC-Dextran 40) is used as a fluorescent probe to study cell processes such as cell permeability, phagocytosis and endocytosis and to study mechanisms of biomolecular drug delivery.
1 of 1
This Item | |||
|---|---|---|---|
| biological source synthetic | biological source synthetic | biological source synthetic (organic) | biological source - |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| form powder | form powder or crystals | form powder | form powder |
| mol wt average mol wt 40,000 | mol wt average mol wt 500,000 | mol wt average mol wt 155,000 | mol wt average mol wt 65,000-85,000 |
| fluorescence λex 550 nm; λem 577 nm±5 nm in 0.1 M Tris pH 8.0 | fluorescence λex 550 nm; λem 575 nm±5 nm in 0.1 M Tris pH 8.0 | fluorescence - | fluorescence - |
| extent of labeling 0.001-0.008 mol/mol TRITC glucose | extent of labeling 0.001-0.008 mol/mol TRITC glucose | extent of labeling ~1 TRITC per 100 glucose residues | extent of labeling ~1 TRITC per 100 glucose residues |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 810156C-1MG | 04061837666490 |
| 800816P-25MG | 04061835533916 |
| 800816P-10MG | 04061835533893 |
| 800816C-10MG | 04061838169617 |
| D2135-250MG | 04061832947198 |
| D2135-100MG | 04061833561652 |
| D2135-1G | 04061833561669 |
| 800816P-200MG | 04061835533909 |
| 800816C-25MG | 04061838169624 |
| 42874-1G | 04061826643372 |
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Can I use paraformaldehyde to fix the tissue and then use fluorescence imaging? For example: Inject Tritc i.v. and then extract the brain tissue. The brain would be fixed in paraformaldehyde to be sliced later and imaged for fluorescence
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