Key Documents

View All Documentation

Anti-Mouse-IgG - Atto 647N antibody produced in goat

Name: Anti-Mouse-IgG - Atto 647N antibody produced in goat
Brand: SIGMA

contains 50% glycerol as stabilizer

Synonym(s):

Atto 647N-Anti-Mouse-IgG antibody produced in goat

Select a Size

Change View

Size/SKU	Availability	Price

About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD06798561

Conjugate:

Atto 647N conjugate

Clone:

polyclonal

Application:

Citations:

Pricing and availability is not currently available.

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

conjugate

Atto 647N conjugate

Quality Segment

100

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

contains

50% glycerol as stabilizer

species reactivity

mouse

concentration

≥0.8 mg/mL IgG

technique(s)

immunofluorescence: 5 μg/mL

fluorescence

λ_ex 647 nm; λ_em 665 nm in PBS

suitability

in accordance for fluorescence

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice. Atto 647N-goat anti-mouse IgG associates with mouse IgGs.

Immunogen

mouse IgG

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.Atto 647N goat anti-rabbit IgG was used as the secondary antibody for immunofluorescene at a concentration of 5μg/ml on cells fixed in 2% formaldehyde.

Physical form

Atto 647 goat anti-mouse IgG (whole molecule) is provided in unit sizes of 1 ml as 1 mg/ml solutions in 0.1 M sodium phosphate, 0.1 M NaCl, pH 7.5, containing 5 mM sodium azide as a preservative.

Analysis Note

unconjugated dye ≤5% of total fluorescence

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item	M4155	A1047	A3438
Sigma-Aldrich 50185 Anti-Mouse-IgG - Atto 647N antibody produced in goat	Sigma-Aldrich M4155 Anti-Mouse IgG (Fab specific) antibody produced in goat	Sigma-Aldrich A1047 Anti-Mouse IgG (γ-chain specific)−Alkaline Phosphatase antibody produced in goat	Sigma-Aldrich A3438 Anti-Mouse IgG (γ-chain specific)−Alkaline Phosphatase antibody produced in goat
technique(s) immunofluorescence: 5 μg/mL	technique(s) Ouchterlony double diffusion: suitable, indirect ELISA: 1:15,000	technique(s) direct ELISA: 1:7,000-1:21,000, western blot: suitable	technique(s) direct ELISA: 1:30,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000
conjugate Atto 647N conjugate	conjugate unconjugated	conjugate alkaline phosphatase conjugate	conjugate alkaline phosphatase conjugate
species reactivity mouse	species reactivity -	species reactivity mouse	species reactivity mouse
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. 2-8°C
clone polyclonal	clone polyclonal	clone polyclonal	clone polyclonal

Still not finding the right product?

Try our Product Selector Tool to narrow your options

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.