Key Documents

Anti-CHIP (C-terminal) antibody produced in rabbit

Name: Anti-CHIP (C-terminal) antibody produced in rabbit
Brand: SIGMA

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-CHIP, carboxy terminus of Hsp70p-interacting protein, Anti-HSPABP2, Anti-NY-CO-7, Anti-STUB1, stip2 homology and U-box containing protein 1, Anti-UBOX1

About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

polyclonal

Application:

Citations:

This product is discontinued. Contact Technical Service for assistance.

Properties

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~35 kDa

species reactivity

human, mouse, rat

concentration

~1 mg/mL

technique(s)

western blot: 1-2 μg/test using HEK-293T cells lysate and mouse brain extract (S2 fraction)

UniProt accession no.

Q9UNE7

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... STUB1(10273)
mouse ... Stub1(56424)
rat ... Stub1(287155)

Description

General description

CHIP (carboxy terminus of Hsp70-interacting protein, also known as STIP1-homology and U-box containing protein 1, STUB1, HSPABP2, NY-CO-7, SDCCAG7, UBOX1), is a dual-function chaperone/E3 ubiquitin ligase. CHIP has 3 functional domains: a N-terminal tetratricopeptide repeat (TPR), a U-box at its C-terminus, and a highly charged internal region. It is located on human chromosome 16p13.3.

Application

Anti-CHIP (C-terminal) antibody produced in rabbit has been used in western blotting and histology to stain testes and ovary sections.

Anti-CHIP (C-terminal) antibody produced in rabbit is suitable for immunoblotting at a working concentration of 1-2 μg/mL using HEK-293T cells lysate and mouse brain extract (S2 fraction).

Biochem/physiol Actions

CHIP (carboxy terminus of Hsp70-interacting protein) plays a crucial role in regulating protein quality control at multiple levels. It ubiquitinates Hsp70, a cytosolic chaperone and enhances refolding of stress damaged proteins. Additionally, it has E3 ubiquitin ligase activity and triggers proteasome degradation of irreversibly damaged proteins to prevent cellular toxicity. The ubiquitination, aggregation and degradation of several proteins involved in neurodegenerative disorders including tau, huntingtin, Cu/Zn SOD1, ataxin-1, and α-synuclein are regulated by CHIP and Hsp70. The aggregation and toxicity of polyglutamine (polyQ) expanded proteins is suppressed by CHIP. It also regulates stress-dependent apoptosis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	B4436	SAB4200403	L9918
Sigma-Aldrich C9243 Anti-CHIP (C-terminal) antibody produced in rabbit	Sigma-Aldrich B4436 Anti-53BP1 (C-terminal) antibody produced in rabbit	Sigma-Aldrich SAB4200403 Anti-EXOSC8 (N-terminal) antibody produced in rabbit	Sigma-Aldrich L9918 Anti-LRRK2 (C-terminal region) antibody produced in rabbit
description ~1 mg/mL, affinity isolated antibody, buffered aqueous solution	description ~1 mg/mL, affinity isolated antibody, buffered aqueous solution	description ~1.0 mg/mL, affinity isolated antibody	description ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
Gene Information human ... STUB1(10273) mouse ... Stub1(56424) rat ... Stub1(287155)	Gene Information human ... TP53BP1(7158) mouse ... Trp53bp1(27223) rat ... Tp53bp1(296099)	Gene Information bovine ... EXOSC8(532080) dog ... EXOSC8(477300) human ... EXOSC8(11340) mouse ... Exosc8(69639)	Gene Information human ... LRRK2(120892) mouse ... Lrrk2(66725) rat ... Lrrk2(300160)
UniProt accession no. Q9UNE7	UniProt accession no. Q12888	UniProt accession no. Q96B26	UniProt accession no. Q5S007
species reactivity human, mouse, rat	species reactivity mouse (predicted), rat (predicted), human	species reactivity mouse, human, rat	species reactivity rat, mouse, human

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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