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Monoclonal Anti-Growth Associated Protein-43 antibody produced in mouse

Name: Monoclonal Anti-Growth Associated Protein-43 antibody produced in mouse
Brand: SIGMA
Price: 552 USD

clone GAP-7B10, ascites fluid

Synonym(s):

Anti-GAP-43

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Size/SKU	Availability	Price
100 μL	Check Cart for Availability	$552.00
0.2 mL	Check Cart for Availability	$727.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00164594

Conjugate:

unconjugated

Clone:

GAP-7B10, monoclonal

Application:

IHC, WB

Citations:

$552.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

GAP-7B10, monoclonal

mol wt

antigen 46 kDa

contains

15 mM sodium azide

species reactivity

hamster, human, feline, rat, chicken, snake, mouse

technique(s)

immunohistochemistry: suitable, western blot: 1:2,000 using newborn rat brain extract

isotype

IgG2a

UniProt accession no.

P17677

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GAP43(2596)
mouse ... Gap43(14432)
rat ... Gap43(29423)

Description

General description

Monoclonal Anti-Growth Associated Protein-43 (mouse IgG2a isotype) is derived from the GAP-7B10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with HPLC-purified GAP-43 from neonatal rat forebrain membranes. Growth Associated Protein 43 (GAP-43) has a very acidic pI (4.3-4.6, depending on the species) and an anomalous behavior in SDS gels such that its reported molecular mass ranges from 43-57 kDa whereas, the primary sequence of the protein and its hydrodynamic behavior indicate a molecular mass of about 24 kDa.

Immunogen

GAP-43 from neonatal rat forebrain membranes.

Application

Monoclonal Anti-Growth Associated Protein-43 antibody produced in mouse has been used in enzyme linked immunosorbent assay (ELISA).

Monoclonal Anti-Growth Associated Protein-43 antibody produced in mouse is suitable for:

immunostaining of tissue sections from the prefrontal cortex and hippocampus of rat pups to recognize an epitope present on kinase C domain in the N terminal of GAP-43 protein
immunohistochemistry at a working dilution of 1:3000 using sections from fetal and adult brains of mice
immunofluorescence at a working dilution of 1:4000 using 8μm sections of mice brain
western blotting using hippocampal lysates from rats

It is also suitable for immunoblotting at a working dilution of 1:2000 using newborn rat brain extract. The antibody is useful in the study of the role and function of GAP-43 during axonogenesis and in the adult nervous system.

Biochem/physiol Actions

Growth Associated Protein 43(GAP-43) precise function in growth cones and synaptic terminals is not known, increased phosphorylation of GAP-43 correlates with exocytosis, depolarization, and treatment of growth cones with NGF, implying that kinase C and possibly other protein kinase-mediated phosphorylation of GAP-43 could be a component of a common response of growth cones to diverse extracellular stimuli. GAP43 associates with the plasma membrane via acylated residues near the amino terminus and with the submembrane fraction of the cytoskeleton, presumably through its highly charged, extended carboxyl terminus, which bears similarities to neurofilament proteins. Monoclonal antibody reacting specifically against GAP-43 is a useful tool in the study of the role and function of GAP-43 during axonogenesis and in the adult nervous system.

Recognizes an epitope present on both kinase C phosphorylated and dephosphorylated forms of GAP-43.

The neural-specific Growth-Associated Protein-43 (GAP-43) is an intracellular phosphoprotein found exclusively in the peripheral and central nervous systems. The axonal growth cones that are involved in the functions of motility and pathfinding during axonogenesis and regeneration contain GAP-43. Phosphorylation of GAP-43 is associated with exocytosis, depolarization and treatment of growth cones with nerve growth factor (NGF).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	C8093	M4278	SAB4300525
Sigma-Aldrich G9264 Monoclonal Anti-Growth Associated Protein-43 antibody produced in mouse	Sigma-Aldrich C8093 Monoclonal Anti-Connexin-43 antibody produced in mouse	Sigma-Aldrich M4278 Monoclonal Anti-MAP1 antibody produced in mouse	Sigma-Aldrich SAB4300525 Anti-GAP43 (Ab-41) antibody produced in rabbit
species reactivity hamster, human, feline, rat, chicken, snake, mouse	species reactivity rat, hamster, bovine, mouse, human	species reactivity mouse, rat	species reactivity rat, human, mouse
antibody form ascites fluid	antibody form ascites fluid	antibody form ascites fluid	antibody form affinity isolated antibody
clone GAP-7B10, monoclonal	clone CXN-6, monoclonal	clone HM-1, monoclonal	clone polyclonal
Gene Information human ... GAP43(2596) mouse ... Gap43(14432) rat ... Gap43(29423)	Gene Information human ... GJA1(2697), GJA1P1(2698) mouse ... Gja1(14609) rat ... Gja1(24392)	Gene Information mouse ... Mtap1a(17754) rat ... Map1a(25152)	Gene Information human ... GAP43(2596)
biological source mouse	biological source mouse	biological source mouse	biological source rabbit
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c