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M0696

Anti-MNK2 (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-GPRK7, Anti-MAP kinase interacting serine/threonine kinase 2, Anti-MKNK2

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Pack SizeSKUAvailabilityPrice
200 μL

Available to ship TODAYfromMILWAUKEE

$584.00
$496.40

About This Item

NACRES:
NA.44
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ICC, IF, IP, WB
Citations:
6

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biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~50 kDa

species reactivity

rat (predicted), human, mouse (predicted)

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

concentration

~1.0 mg/mL

technique(s)

immunocytochemistry: 2-5 μg/mL using paraformadehyde fixed HEK-293T cells transfected with human MNK2, immunoprecipitation (IP): 2-4 μg using lysates of HEK-293T cells transfected with human MNK2, indirect immunofluorescence: suitable, western blot: 1-2 μg/mL using lysates of HEK-293T cells transfected with human MNK2

UniProt accession no.

Q9HBH9

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MKNK2(2872)
mouse ... Mknk2(17347)
rat ... Mknk2(299618)

General description

MNK2 is a serine threonine kinase that phosphorylates endogenous eIF4E and thereby regulates protein translation and cell growth. This kinase inhibits apoptotic responses induced by arsenic trioxide. MNK2 has two distinct splice forms, namely; MNK2a and MNK2b. The localization and function of MNK2 splice variants are directed by their N and C termini . Anti-MNK2 (N-terminal) antibody is specific for human MNK2. The antibody is also expected to react with mouse and rat MNK2. In immunoblotting, staining of the MNK2 band is specifically inhibited by the immunizing peptide.

Immunogen

synthetic peptide corresponding to amino acids 51-64 of human MNK2 (corresponding to isoforms 1 and 2), conjugated to KLH via a C-terminal added cysteine residue. The corresponding sequence is conserved in human, rat, and mouse.

Application

Anti-MNK2 (N-terminal) antibody is suitable for use in immunocytochemistry (2-5 μg/mL using paraformaldehyde fixed, MNK2-transfected, HEK-293T cells) and indirect immunofluorescence. The antibody may also be used for immunoprecipitation (2-4 μg) and western blot (1-2 μg/mL) using lysates of HEK-293T cells transfected with human MNK2.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anti-MNK2 (N-terminal) antibody produced in rabbit ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

M0696

Anti-MNK2 (N-terminal) antibody produced in rabbit

Anti-MNK1 antibody produced in rabbit ~1.4 mg/mL, affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

M3946

Anti-MNK1 antibody produced in rabbit

Anti-MKNK2 antibody produced in rabbit affinity isolated antibody

Sigma-Aldrich

SAB2101483

Anti-MKNK2 antibody produced in rabbit

ANTI-MNK2 (MKNK2)(N-TERMINAL) antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

Sigma-Aldrich

SAB1306024

ANTI-MNK2 (MKNK2)(N-TERMINAL) antibody produced in rabbit

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

-

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

-

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

technique(s)

immunocytochemistry: 2-5 μg/mL using paraformadehyde fixed HEK-293T cells transfected with human MNK2, indirect immunofluorescence: suitable, immunoprecipitation (IP): 2-4 μg using lysates of HEK-293T cells transfected with human MNK2, western blot: 1-2 μg/mL using lysates of HEK-293T cells transfected with human MNK2

technique(s)

western blot: 1-2 μg/mL using lysates of HEK-293T cells transfected with human Mnk1

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

western blot: 1:1000

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000140239
0000133859

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Related Content

Data Sheet - M0696

Instructions

Senthilmurugan Ramalingam et al.
Cancers, 11(3) (2019-03-06)
Currently, there are no effective therapies for patients with triple-negative breast cancer (TNBC), an aggressive and highly metastatic disease. Activation of eukaryotic initiation factor 4E (eIF4E) by mitogen-activated protein kinase (MAPK)-interacting kinases 1 and 2 (Mnk1/2) play a critical role
Y Shi et al.
Oncogene, 32(2), 190-197 (2012-03-01)
When mTOR inhibitor rapalogs prevent cap-dependent translation of cell-cycle proteins like c-myc, continuing tumor cell growth depends on cap-independent translation, which is mediated by internal ribosome entry sites (IRESes) located in the 5'-UTR (untranslated region) of transcripts. To investigate if
Gert C Scheper et al.
Molecular and cellular biology, 23(16), 5692-5705 (2003-08-05)
The cap-binding eukaryotic initiation factor eIF4E is phosphorylated by the mitogen-activated protein (MAP) kinase-interacting kinases (Mnk's). Three forms of the Mnk's exist in human cells: Mnk1, Mnk2a, and Mnk2b. These last two are derived from the same gene by alternative
View All Related Papers

Global Trade Item Number

SKUGTIN
M0696-200UL04061838031624

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