Unless otherwise stated, this product is intended for research use only. It is not suitable for any other purpose, which includes, but is not limited to, unauthorized commercial use, in vitro diagnostic use, or therapeutic use in humans or animals.
M2272
Melittin from honey bee venom
≥85% (HPLC)
Melittin from honey bee venom
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 mg | Please contact Customer Service for Availability | $113.00 | |
| 5 mg | Please contact Customer Service for Availability | $385.00 | |
| 25 mg | Please contact Customer Service for Availability | $1,200.00 |
About This Item
Empirical Formula (Hill Notation):
C131H229N39O31
CAS Number:
Molecular Weight:
2846.46
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352200
MDL number:
biological source
animal (Apis mellifera)
Quality Level
assay
≥85% (HPLC)
form
lyophilized powder
mol wt
~_2.8 kDa
solubility
water: 5.00-5.20 mg/mL, clear, colorless to faintly yellow
antibiotic activity spectrum
Gram-negative bacteria, Gram-positive bacteria
foreign activity
Phospholipase A2 ≤0.5%
mode of action
cell membrane | interferes
storage temp.
−20°C
SMILES string
CC[C@H](C)[C@H](NC(=O)CN)C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CO)C(=O)N[C@@H](Cc2c[nH]c3ccccc23)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(N)=O)C(N)=O
InChI
1S/C131H229N39O31/c1-23-71(16)102(163-97(176)60-135)122(194)146-62-98(177)148-74(19)109(181)164-100(69(12)13)124(196)160-88(55-65(4)5)116(188)155-84(41-30-33-51-134)115(187)165-101(70(14)15)125(197)161-90(57-67(8)9)118(190)168-106(77(22)173)128(200)169-105(76(21)172)123(195)147-63-99(178)150-92(58-68(10)11)129(201)170-54-36-44-94(170)121(193)149-75(20)108(180)158-89(56-66(6)7)117(189)166-104(73(18)25-3)127(199)162-93(64-171)120(192)159-91(59-78-61-145-80-38-27-26-37-79(78)80)119(191)167-103(72(17)24-2)126(198)157-83(40-29-32-50-133)111(183)154-85(42-34-52-143-130(139)140)112(184)152-82(39-28-31-49-132)110(182)153-86(43-35-53-144-131(141)142)113(185)156-87(46-48-96(137)175)114(186)151-81(107(138)179)45-47-95(136)174/h26-27,37-38,61,65-77,81-94,100-106,145,171-173H,23-25,28-36,39-60,62-64,132-135H2,1-22H3,(H2,136,174)(H2,137,175)(H2,138,179)(H,146,194)(H,147,195)(H,148,177)(H,149,193)(H,150,178)(H,151,186)(H,152,184)(H,153,182)(H,154,183)(H,155,188)(H,156,185)(H,157,198)(H,158,180)(H,159,192)(H,160,196)(H,161,197)(H,162,199)(H,163,176)(H,164,181)(H,165,187)(H,166,189)(H,167,191)(H,168,190)(H,169,200)(H4,139,140,143)(H4,141,142,144)/t71-,72-,73-,74-,75-,76+,77+,81-,82-,83-,84-,85-,86-,87-,88-,89-,90-,91-,92-,93-,94-,100-,101-,102-,103-,104-,105-,106-/m0/s1
InChI key
VDXZNPDIRNWWCW-JFTDCZMZSA-N
General description
Chemical structure: peptide
Melittin is hydrophobic in nature except for a region with Lys-Arg-Lys-Arg sequence near C-terminal end. This structural characteristic makes melittin a highly surface-active and a powerful, direct haemolytic agent.† The encoded protein containins 26 amino acids. Monomeric form of melittin has a molecular weight of 2,840 Daltons and tetrameric form has molecular weight of approximately 12,500 Daltons.
Application
Melittin from honey bee venom has been used:
- In 3-(4, 5-dimethyl thiazol-2-yl)-2,5diphenyl tetrazolium bromide (MTT) assay to determine its cytotoxicity effect on the growth of human cell lines.
- To study the anti-microbial activity of melittin on the growth of Borrelia burgdorferi in in vitro conditions.
- As a positive control in hemolysis assay and as a cytotoxic agent against HeLa cells.[1]
Biochem/physiol Actions
Binds calmodulin in a Ca2+-dependent manner; inhibits Na+-K+-ATPase.
Melittin acts as an anti-coagulating protein by increasing the time of blood clotting in vitro. Melittin inhibits the activity of S100 calcium-binding protein B (S100B) and plays a vital role in Epilepsy treatment.
Other Notes
The principle hemolytic component of honeybee venom.
1 of 1
This Item | |||
|---|---|---|---|
| assay ≥85% (HPLC) | assay - | assay ≥97% (HPLC) | assay - |
| biological source animal (Apis mellifera) | biological source - | biological source - | biological source human plasma |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 300 |
| form lyophilized powder | form lyophilized powder | form - | form powder |
| mol wt ~_2.8 kDa | mol wt ~_2.8 kDa | mol wt - | mol wt α-chain 63.5 kDa, β-chain 56 kDa, γ chain 47 kDa (about 4% carbohydrate content), soluble dimer 340 kDa |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
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signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Oral
Storage Class
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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The antimicrobial agent melittin exhibits powerful in vitro inhibitory effects on the Lyme disease spirochete.
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Clinical Infectious Diseases, suppl 1, S48-S51 (1997)
Selective cytotoxicity Assay in anticancer drug of Melittin Isolated from
Bee Venom (Apis cerana indica) to several human cell lines: HeLa,
WiDr and Vero.
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Journal of Chemical and Pharmaceutical Sciences
, 9:4, 2674-2676 (2016)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| M2272-25MG | 04061834041757 |
| M2272-5MG | 04061834041764 |
| M2272-1MG | 04061834041740 |
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