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For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
P9620
Puromycin dihydrochloride
Ready Made Solution, from Streptomyces alboniger, 10 mg/mL in H2O, suitable for cell culture
Synonym(s):
3′-(L-α-Amino-p-methoxyhydrocinnamamido)-3′-deoxy-N,N-dimethyladenosine dihydrochloride, Stylomycin dihydrochloride
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 10 mL | Available to ship TODAYfromMilliporeSigma Canada Ltd. | $603.00 |
About This Item
biological source
Streptomyces alboniger
Quality Level
type
for cell culture
assay
>98% (HPLC)
form
ready-to-use solution
storage condition
(Store in cool place. Keep container tightly closed in a dry and well -ventilated place. )
concentration
10 mg/mL in H2O
technique(s)
cell culture | mammalian: suitable
color
colorless to yellow
solubility
H2O: soluble 10 mg/mL
antibiotic activity spectrum
Gram-positive bacteria, neoplastics, parasites
application(s)
advanced drug delivery
mode of action
protein synthesis | interferes
shipped in
wet ice
storage temp.
−20°C
SMILES string
Cl.Cl.COc1ccc(C[C@H](N)C(=O)N[C@H]2[C@@H](O)[C@@H](O[C@@H]2CO)n3cnc4c(ncnc34)N(C)C)cc1
InChI
1S/C22H29N7O5.2ClH/c1-28(2)19-17-20(25-10-24-19)29(11-26-17)22-18(31)16(15(9-30)34-22)27-21(32)14(23)8-12-4-6-13(33-3)7-5-12;;/h4-7,10-11,14-16,18,22,30-31H,8-9,23H2,1-3H3,(H,27,32);2*1H/t14-,15+,16+,18+,22+;;/m0../s1
InChI key
MKSVFGKWZLUTTO-FZFAUISWSA-N
General description
Puromycin′s structural similarity to the amino acid-bearing end of tRNA allows it to enter the ribosome during protein synthesis, bind to the nascent polypeptide chain, and halt chain elongation. Widely used in cell culture, puromycin serves as a selective agent for cells that have been transformed with a gene conferring resistance to puromycin.
Application
Biochem/physiol Actions
Mode of Resistance: Puromycin acetyltransferase is an effective resistance gene.
Antimicrobial Spectrum: This product is active against gram-positive microorganisms, less active against acid-fast bacilli and more weakly active against gram-negative microorganisms. Puromycin can prevent growth of bacteria, protozoa, algae and mammalian cells and acts quickly, killing 99% of cells within 2 days.
Features and Benefits
- High-quality antibiotic suitable for multiple research applications
- Antibiotic and protein synthesis inhibitor
- Effective against prokaryotic and eukaryotic cells
- Inhibits protein synthesis by interfering with RNA function
- Commonly used as a selective agent in cell biology research
Preparation Note
Other Notes
1 of 1
This Item | |||
|---|---|---|---|
| mode of action protein synthesis | interferes | mode of action protein synthesis | interferes | mode of action protein synthesis | interferes | mode of action DNA synthesis | interferes, enzyme | inhibits |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) - |
| antibiotic activity spectrum Gram-positive bacteria, parasites, neoplastics | antibiotic activity spectrum Gram-positive bacteria, neoplastics, parasites | antibiotic activity spectrum Gram-positive bacteria, neoplastics, parasites | antibiotic activity spectrum neoplastics |
| Quality Level 300 | Quality Level 300 | Quality Level 300 | Quality Level 200 |
| application(s) advanced drug delivery | application(s) agriculture | application(s) - | application(s) - |
| assay >98% (HPLC) | assay ≥98% (HPLC) | assay ≥98% (HPLC) | assay 95% (HPLC) |
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Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, multi-purpose combination respirator cartridge (US)
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Protocols
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Articles
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| P9620-10ML | 04061838446862 |
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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