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GenElute^™ Plasmid Midiprep Kit

Name: GenElute<SUP>™</SUP> Plasmid Midiprep Kit
Brand: SIGMA
Price: 491 CAD

sufficient for 35 purifications

Synonym(s):

Gen Elute, GenElute^™ Plasmid Kit, plasmid extraction kit, plasmid isolation kit, plasmid purification kit

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Pack Size	SKU	Availability	Price
1 kit		Please contact Customer Service for Availability	$491.00

About This Item

NACRES:

NA.52

UNSPSC Code:

41105501

$491.00

Please contact Customer Service for Availability

Technical Service

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Properties

usage

sufficient for 35 purifications

Quality Level

200

greener alternative product characteristics

Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

technique(s)

DNA purification: suitable

test parameters

: ~45 min kit run time, sample volume: 5-40 mL bacterial culture

greener alternative category

Aligned,

storage temp.

15-25°C

Description

General description

Click here to view the kit protocol

The GenElute Plasmid Midiprep Kit offers a simple, rapid, cost-effective method for isolating plasmid DNA from recombinant E. coli cultures. By combining silica- binding technology and the convenience of a spin column format, up to 300 μg of plasmid DNA can be recovered from 20-40 ml of Luria Broth (LB) in about 45 minutes. Note that actual yield and optimal volume of culture to use depend on the plasmid and the culture medium.

An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto silica in the presence of high salts. Contaminants are then removed by a spin-wash step. Finally, the bound DNA is eluted in water or Tris-EDTA buffer.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Application

The DNA is ready for immediate use in such downstream applications as restriction enzyme digestion, cloning, PCR, transcription and automated sequencing.

The recovered plasmid DNA is predominately in its supercoiled form. There is no visual evidence of genomic DNA or RNA contamination detected by agarose gel electrophoresis. The DNA is ready for immediate use in downstream applications such as restriction digestion, ligation, sequencing, PCR, and transfection.

Biochem/physiol Actions

Bacterial cells are harvested via centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorbtion of DNA onto silca in the presence of high salts. Contaminants are then removed by a simple wash step. Finally, the bound DNA is eluted in water or Tris-EDTA buffer. The recovered plasmid DNA is predominately in its supercoiled form. There is no visual evidence of genomic DNA or RNA contamination.

Other Notes

GenElute Plasmid Midi- and Maxiprep Kits offer a simple, rapid and cost-effective method for isolating plasmid DNA from E. coli cultures. These kits combine silica-based membrane technology with the convenience of a spin column format and deliver up to 350 μg (Midi) and 1.2 mg (Maxi) yield of high-copy plasmid DNA.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

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This Item	PLED35	NA0200	PLN70
Sigma-Aldrich PLD35 GenElute^™ Plasmid Midiprep Kit	Sigma-Aldrich PLED35 GenElute^™ Endotoxin-free Plasmid Midiprep Kit	Sigma-Aldrich NA0200 GenElute^™ HP Plasmid Midiprep Kit	Sigma-Aldrich PLN70 GenElute^™ Plasmid Miniprep Kit
technique(s) DNA purification: suitable	technique(s) DNA purification: suitable	technique(s) DNA purification: suitable	technique(s) DNA purification: suitable
usage sufficient for 35 purifications	usage sufficient for 35 purifications	usage sufficient for 25 purifications	usage sufficient for 70 purifications
greener alternative category Aligned,	greener alternative category Aligned,	greener alternative category , Aligned	greener alternative category , Aligned
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
sustainability Greener Alternative Product	sustainability Greener Alternative Product	sustainability Greener Alternative Product	sustainability Greener Alternative Product
test parameters : ~45 min kit run time, sample volume: 5-40 mL bacterial culture	test parameters : <2 hr kit run time for up to 12 preps, sample volume: 25-200 mL bacterial culture	test parameters sample volume: 1-5 mL bacterial culture	test parameters : <30 min kit run time, sample volume: 1-5 mL