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A2831

Monoclonal Anti-DNP−Alkaline Phosphatase Conjugate antibody produced in mouse

clone SPE-7, purified immunoglobulin, buffered aqueous glycerol solution

Synonym(s):

Anti-Dinitrophenyl, Monoclonal Anti-DNP

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0.5 mL
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$1,200.00

About This Item

NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
alkaline phosphatase conjugate
Clone:
SPE-7, monoclonal
Application:
DB, ELISA (d)
Citations:
5

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biological source

mouse

Quality Segment

200

conjugate

alkaline phosphatase conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

SPE-7, monoclonal

form

buffered aqueous glycerol solution

technique(s)

direct ELISA: 1:25,000 using DNP-BSA, dot blot: 1:180,000 (chemiluminescence)

isotype

IgE

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Dinitrophenyl (DNP) is a hapten that is used for labeling primary or secondary probes in immunological analyses. Thus, anti-DNP antibody conjugated to a detectable substrate is a useful tool for the study of target proteins. Mouse monoclonal anti-DNP-alkaline phosphatase conjugate antibody is specific for DNP.

Immunogen

DNP-KLH conjugate

Application

Mouse monoclonal anti-DNP-alkaline phosphatase conjugate antibody can be used for ELISA (1:25,000) and dot blot (1:180,000) assays.
The antibody may be used for experimental induction of controlled allergic reactions or as a source of mouse IgE.
The antibody may be used for experimental induction of controlled allergic reactions or as a source of mouse IgE.To induce systemic anaphylaxis, mice were injected with 3 μg of anti-DNP mouse IgE and then given by IV 500 μg of DNP-BSA twenty hours later. To induce local anaphylaxis, mice were injected intradermally with 10ng of anti-DNP mouse IgE and then challenged with an i.v. injection of 500 μg DNP-BSA.
Used to induce controlled allergic reactions or as a source of mouse IgE.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% BSA, and 50% glycerol and 15 mM Sodium azide

Analysis Note

Monoclonal Anti-DNP is tested to ensure minimal reactivity against bovine serum albumin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item
D8406A9469A5477
Monoclonal Anti-DNP−Alkaline Phosphatase Conjugate antibody produced in mouse clone SPE-7, purified immunoglobulin, buffered aqueous glycerol solution

Sigma-Aldrich

A2831

Monoclonal Anti-DNP−Alkaline Phosphatase Conjugate antibody produced in mouse

Anti -Dinitrophenyl antibody, Mouse monoclonal IgE isotype, ~1 mg/mL, clone SPE-7, affinity purified immunoglobulin, buffered aqueous solution

Sigma-Aldrich

D8406

Anti -Dinitrophenyl antibody, Mouse monoclonal

Monoclonal ANTI-FLAG® M2-Alkaline Phosphatase antibody produced in mouse clone M2, purified immunoglobulin, buffered aqueous glycerol solution

Sigma-Aldrich

A9469

Monoclonal ANTI-FLAG® M2-Alkaline Phosphatase antibody produced in mouse

Anti-HA−Alkaline Phosphatase antibody, Mouse monoclonal clone HA-7, purified from hybridoma cell culture

Sigma-Aldrich

A5477

Anti-HA−Alkaline Phosphatase antibody, Mouse monoclonal

description

clone SPE-7, purified immunoglobulin, buffered aqueous glycerol solution

description

IgE isotype, ~1 mg/mL, clone SPE-7, affinity purified immunoglobulin, buffered aqueous solution

description

clone M2, purified immunoglobulin, buffered aqueous glycerol solution

description

clone HA-7, purified from hybridoma cell culture

clone

SPE-7, monoclonal

clone

SPE-7, monoclonal

clone

M2, monoclonal

clone

HA-7, monoclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

conjugate

alkaline phosphatase conjugate

conjugate

unconjugated

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

antibody form

purified immunoglobulin

antibody form

affinity purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

technique(s)

direct ELISA: 1:25,000 using DNP-BSA, dot blot: 1:180,000 (chemiluminescence)

technique(s)

indirect ELISA: 0.05-0.1 μg/mL using using 0.5 μg/well DNP-BSA as the coating antigen and 1 μg/mL Monoclonal Anti-Mouse IgE, Peroxidase conjugate as the detector antibody.

technique(s)

indirect ELISA: 1:20,000

technique(s)

western blot: 1:4,000 using extracts of mammalian cells expressing HA tagged fusion proteins

form

buffered aqueous glycerol solution

form

buffered aqueous solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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