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Monoclonal Anti-DNP−Alkaline Phosphatase Conjugate antibody produced in mouse

Name: Monoclonal Anti-DNP−Alkaline Phosphatase Conjugate antibody produced in mouse
Brand: SIGMA
Price: 1200 USD
Availability: InStock

clone SPE-7, purified immunoglobulin, buffered aqueous glycerol solution

Synonym(s):

Anti-Dinitrophenyl, Monoclonal Anti-DNP

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Size/SKU	Availability	Price
0.5 mL	Available to ship TODAYfromMILWAUKEE	$1,200.00

About This Item

NACRES:

NA.46

UNSPSC Code:

12352203

Conjugate:

alkaline phosphatase conjugate

Clone:

SPE-7, monoclonal

Application:

DB, ELISA (d)

Citations:

$1,200.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

alkaline phosphatase conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

SPE-7, monoclonal

form

buffered aqueous glycerol solution

technique(s)

direct ELISA: 1:25,000 using DNP-BSA, dot blot: 1:180,000 (chemiluminescence)

isotype

IgE

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Description

General description

Dinitrophenyl (DNP) is a hapten that is used for labeling primary or secondary probes in immunological analyses. Thus, anti-DNP antibody conjugated to a detectable substrate is a useful tool for the study of target proteins. Mouse monoclonal anti-DNP-alkaline phosphatase conjugate antibody is specific for DNP.

Immunogen

DNP-KLH conjugate

Application

Mouse monoclonal anti-DNP-alkaline phosphatase conjugate antibody can be used for ELISA (1:25,000) and dot blot (1:180,000) assays.

The antibody may be used for experimental induction of controlled allergic reactions or as a source of mouse IgE.

The antibody may be used for experimental induction of controlled allergic reactions or as a source of mouse IgE.To induce systemic anaphylaxis, mice were injected with 3 μg of anti-DNP mouse IgE and then given by IV 500 μg of DNP-BSA twenty hours later. To induce local anaphylaxis, mice were injected intradermally with 10ng of anti-DNP mouse IgE and then challenged with an i.v. injection of 500 μg DNP-BSA.

Used to induce controlled allergic reactions or as a source of mouse IgE.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl₂, 1% BSA, and 50% glycerol and 15 mM Sodium azide

Analysis Note

Monoclonal Anti-DNP is tested to ensure minimal reactivity against bovine serum albumin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	A2189	A2306	A7554
Sigma-Aldrich A2831 Monoclonal Anti-DNP−Alkaline Phosphatase Conjugate antibody produced in mouse	Sigma-Aldrich A2189 Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase, Mouse monoclonal	Sigma-Aldrich A2306 Monoclonal Anti-Rabbit Immunoglobulins−Alkaline Phosphatase antibody produced in mouse	Sigma-Aldrich A7554 Monoclonal Anti-Bovine IgG−Alkaline Phosphatase antibody produced in mouse
description clone SPE-7, purified immunoglobulin, buffered aqueous glycerol solution	description clone MB-11, purified from hybridoma cell culture	description clone RG-16, purified immunoglobulin, buffered aqueous glycerol solution	description clone BG-18, purified immunoglobulin, buffered aqueous glycerol solution
antibody form purified immunoglobulin	antibody form purified from hybridoma cell culture	antibody form purified immunoglobulin	antibody form purified immunoglobulin
conjugate alkaline phosphatase conjugate	conjugate alkaline phosphatase conjugate	conjugate alkaline phosphatase conjugate	conjugate alkaline phosphatase conjugate
biological source mouse	biological source mouse	biological source mouse	biological source mouse
clone SPE-7, monoclonal	clone MB-11, monoclonal	clone RG-16, monoclonal	clone BG-18, monoclonal
form buffered aqueous glycerol solution	form buffered aqueous glycerol solution	form buffered aqueous glycerol solution	form buffered aqueous glycerol solution
technique(s) direct ELISA: 1:25,000 using DNP-BSA, dot blot: 1:180,000 (chemiluminescence)	technique(s) direct ELISA: 1:35,000, dot blot: 1:30,000 using 20 ng of human IgM/dot (chemiluminescence), dot blot: 1:20,000 using 20 ng of human IgM/dot	technique(s) western blot: 1:160,000-1:320,000 using detecting β-actin in total cell extract of HeLa cells (5-10 μg per well), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160 using human tonsil and Cat. No. I8635 as the primary antibody, direct ELISA: 1:40,000	technique(s) direct ELISA: 1:25,000, dot blot: suitable, western blot (chemiluminescent): 1:100,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:75 using using rabbit spleen sections and bovine anti-rabbit IgG

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Storage Class

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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