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Monoclonal Anti-Alkaline Phosphatase, Human Placental antibody produced in mouse

Name: Monoclonal Anti-Alkaline Phosphatase, Human Placental antibody produced in mouse
Brand: SIGMA
Price: 598 USD
Availability: InStock

clone 8B6, ascites fluid

Synonym(s):

Monoclonal Anti-Alkaline Phosphatase, Human Placental, Anti-Phosphatase, Alkaline, Human Placental, PLAP

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Size/SKU	Availability	Price
0.2 mL	Available to ship TODAYfromMILWAUKEE	$598.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162106

Conjugate:

unconjugated

Clone:

8B6, monoclonal

Application:

ELISA (i), ICC, IHC (f), IHC (p), RIA, WB

Citations:

$598.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

8B6, monoclonal

contains

15 mM sodium azide

species reactivity

human

technique(s)

immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:4,000 using human placenta, immunohistochemistry (frozen sections): suitable, indirect ELISA: suitable using whole cells, radioimmunoassay: suitable using radio-immunolocalization of xenografts of human cell lines expressing PLAP in nude mice², western blot: suitable

isotype

IgG2a

UniProt accession no.

P05187

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ALPP(250)

Description

General description

In SDS gels, the product reacts with both Regan and Nagao isozymes of human placental alkaline phosphatase (hPLAP, 130 kDa, 67/130 kDa). By RIA, the antibody binds to hPLAP with an affinity constant of 5 × 10⁹ LM^-1. It does not react with PLAP-like enzymes.

Monoclonal Anti-Human Placental Alkaline Phosphatase (mouse IgG2a isotype) is derived from the 8B6 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Placental alkaline phosphatase (PLAP) or ALPP is a phosphomonoesterase. It is expressed in the placenta and endometrium. The PLAP gene is localized on human chromosome 2q37.1. Small amounts of hPLAP are found in the endocervix, fallopian tubes and lung. Very small amounts of heat-stable AP resembling hPLAP (hPLAPlike AP) are expressed in the testis, thymus and in rare colon epithelial cells.

Immunogen

Human epidermoid carcinoma cell line expressing placental alkaline phosphatase.

Application

Anti-Alkaline phosphatase human placental antibody coupled to agarose was packed in a column and used to purify alkaline-phosphatase conjugated FGF receptor out of conditioned media from COS-7 cells. Column was washed with 1M NaCl.

Cultured pancreatic islet cells were fixed with cold 4% paraformaldhyde at room temperature for 10 minutes and incubated with mouse monoclonal anti-Alkaline Phosphatase, Human Placental antibody at 1:500. Mouse monoclonal anti-Alkaline Phosphatase, Human Placental antibody was used to detect a unique molecular probe that consisted of the FGFR extracellular domain expressed as a chimera with human PLAP.

Monoclonal Anti-Alkaline Phosphatase, Human Placental antibody produced in mouse has been used in:

enzyme-linked immunosorbent assay (ELISA)
immunocytochemistry
radio-immunolocalization
western blot
immunofluorescence
immunohistochemistry

Biochem/physiol Actions

Placental alkaline phosphatase (PLAP) or ALPP is a hydrolase, which is involved in the hydrolysis of phosphomonoesters. It has been shown to be a biomarker for ovarian cancer. The protein is expressed in carcinomas such as ovarian adenocarcinoma, dysgerminoma, serous cystadenocarcinoma and undifferentiated carcinoma. Although hPLAP has been analyzed as a potential tumor marker, it has not been widely used in the routine clinical laboratory because of its low overall specificity due to heat-inactivation, cross-reactivity with intestinal AP and L-phenylalanine sensitivity. The use of monoclonal antibodies as reagents has greatly improved the sensitivity, reproducibility and accuracy of the tests used to distinguish placental isozymes from their tumor cell counterparts. Thus, monoclonal anti-alkaline phosphatase may be useful as diagnostic tools for tumor detection.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	SAB4700990	SAB5500164	A3312
Sigma-Aldrich A2951 Monoclonal Anti-Alkaline Phosphatase, Human Placental antibody produced in mouse	Sigma-Aldrich SAB4700990 Monoclonal Anti-Placental Alkaline Phosphatase antibody produced in mouse	Sigma-Aldrich SAB5500164 Anti-PLAP antibody, Rabbit monoclonal	Sigma-Aldrich A3312 Anti-Human IgG (γ-chain specific), F(ab′)₂ fragment−Alkaline Phosphatase antibody produced in goat
description clone 8B6, ascites fluid	description clone H7E8	description recombinant, expressed in proprietary host, clone SP15, tissue culture supernatant	description affinity isolated antibody, buffered aqueous glycerol solution
biological source mouse	biological source mouse	biological source rabbit	biological source goat
clone 8B6, monoclonal	clone H7E8, monoclonal	clone SP15, monoclonal	clone polyclonal
Gene Information human ... ALPP(250)	Gene Information human ... ALPP(250)	Gene Information human ... ALPP(250)	Gene Information -
antibody form ascites fluid	antibody form purified immunoglobulin	antibody form tissue culture supernatant	antibody form affinity isolated antibody
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate alkaline phosphatase conjugate
species reactivity human	species reactivity -	species reactivity human (tested)	species reactivity human

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Storage Class

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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