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Anti-AKR1C3 antibody, Mouse monoclonal

Name: Anti-AKR1C3 antibody, Mouse monoclonal
Brand: SIGMA
Price: 198 USD
Availability: InStock

clone NP6.G6.A6, purified from hybridoma cell culture

Synonym(s):

Anti-DD-3, Anti-DD3, Anti-HA1753

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Size/SKU	Availability	Price
25 μL	Available to ship TODAYfromMILWAUKEE	$198.00
200 μL	Available to ship TODAYfromMILWAUKEE	$761.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD06798693

Conjugate:

unconjugated

Clone:

NP6.G6.A6, monoclonal

Application:

ARR, ELISA (i), IHC, WB

Citations:

$198.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

NP6.G6.A6, monoclonal

form

buffered aqueous solution

mol wt

antigen ~38 kDa

species reactivity

human

packaging

antibody small pack of 25 μL

technique(s)

immunohistochemistry: suitable, indirect ELISA: suitable, microarray: suitable, western blot: 0.25-0.5 μg/mL using cytosolic fraction extract of A549 human lung carcinoma cell

isotype

IgG1

UniProt accession no.

P42330

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... AKR1C3(8644)

Description

General description

AKR1C3, aldo-keto reductase family 1, member C3 (3-α hydroxysteroid dehydrogenase, type II), encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of

Anti-AKR1C3 antibody, Mouse monoclonal (humanα - hydroxysteroid dehydrogenase, type 2,3; 17β- hydroxysteroid dehydrogenase, type 5) (mouse IgG1 isotype) is derived from the hybridoma NP6.G6. A6 produced by the fusion of mouse myeloma cells (SP20 cells) and splenocytes from BALB/c mice immunized with human AKR1C3 protein. Oxidoreduction of natural and foreign substrates is performed by three enzymes superfamilies, one of them being the AKRs (aldo-keto reductases) family. This family contains 114 proteins that are expressed in prokaryotes and eukaryotes and is divided into 14 subfamilies (AKR1-AKR14). The AKR1 family is the largest among the different fourteen families and contains the aldose reductases, aldehyde reductases, hydroxysteroid dehydrogenases, and steroid 5 β-reductases. AKR1Cs are expressed in many tissues.

Immunogen

human AKR1C3 protein.

Application

Anti-AKR1C3 antibody, Mouse monoclonal has been used in

western blotting
immunohistochemistry
enzyme linked immunoassay (ELISA).

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Immunohistochemistry on human placentas were performed using monoclonal mouse anti-AKR1C3 at a dilution of 1:100 or western blot at 1:500.

Mouse monoclonal clone NP6.G6.A6 anti-AKR1C3 antibody used to tag aldo-keto reductase 1C3 for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of aldo-keto reductase 1C3 in the regulation of the androgen 5α-dihydrotestosterone (DHT) and 3α-diol compound levels in vivo.

Biochem/physiol Actions

In humans AKR1C3 (aldo-keto reductase family 1 member C3) including AKR1C1,2 and 4, catalyze the reduction of the androgen 5α-dihydrotestosterone (DHT) into inactive 3β or 3α-androstane diol (3α/β-Diol). AKR1C1-4 isoforms also display 3α[17β]-hydroxysteroid oxidase activity using 3α-Diol as a substrate, in vitro. In non-small cell lung carcinoma, AKR1Cs expression is dramatically increased. Due to their product profile and discrete tissue localization, they may regulate the level of active androgens, estrogens, and progestins in target tissues.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	A2859	A1106	P6874
Sigma-Aldrich A6229 Anti-AKR1C3 antibody, Mouse monoclonal	Sigma-Aldrich A2859 Monoclonal Anti-ATG5 antibody produced in mouse	Sigma-Aldrich A1106 Anti-ATM antibody,Mouse monoclonal	Sigma-Aldrich P6874 Anti-p53 antibody, Mouse monoclonal
description clone NP6.G6.A6, purified from hybridoma cell culture	description clone ATG5-18, purified immunoglobulin, buffered aqueous solution	description clone MAT3-4G10/8, purified from hybridoma cell culture	description clone DO-1, purified from hybridoma cell culture
species reactivity human	species reactivity human, mouse, rat	species reactivity human, mouse	species reactivity human
biological source mouse	biological source mouse	biological source mouse	biological source mouse
Gene Information human ... AKR1C3(8644)	Gene Information human ... ATG5(9474) mouse ... Atg5(11793) rat ... Atg5(365601)	Gene Information human ... ATM(472) mouse ... Atm(11920)	Gene Information human ... TP53(7157)
clone NP6.G6.A6, monoclonal	clone ATG5-18, monoclonal	clone MAT3-4G10/8, monoclonal	clone DO-1, monoclonal
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified from hybridoma cell culture	antibody form purified immunoglobulin

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