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A8312

m-Aminophenylboronic acid–Agarose

aqueous suspension

Synonym(s):

(3-aminophenyl)boronic acid-Agarose, 3-Aminophenylboronic acid–Agarose, m-APBA-Agarose, m-Aminophenylboronic acid resin

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Pack SizeSKUAvailabilityPrice
5 mL
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$345.00

About This Item

UNSPSC Code:
12161501
PubChem Substance ID:
329770955
NACRES:
NA.32
MDL number:
MFCD00801303

$345.00

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form

aqueous suspension

Quality Segment

300

extent of labeling

40-80 μmol per mL

matrix

6% beaded agarose

matrix activation

epichlorohydrin

matrix attachment

through amino to carboxyls of EDTA

matrix spacer

9 atoms

capacity

≥8 mg/mL binding capacity (peroxidase Type VI)

storage temp.

2-8°C

SMILES string

[X]Nc1cc(ccc1)B(O)O

General description

m-Aminophenylboronic acid (m-APBA) immobilized on an agarose gel is useful in immunoglobulins capture, but also leads to non-specific interactions.[1] m-APBA is a boronate affinity matrix that is effectively used for affinity purification of monoclonal antibodies.[2]

Application

m-Aminophenylboronic acid-Agarose has been used:
  • as a component of the column for the purification of Escherichia coli TOP10F cells
  • with cell lysates of various cells for detection of PARylated (Poly(ADP-ribos)ylation) proteins.
  • in the affinity purification of horseradish peroxidase (HRPO)[2]
  • in the glycated human serum albumin (gHSA) purification[3]

Physical form

Suspension in water containing 0.002% chlorhexidine diacetate.

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This Item
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m-Aminophenylboronic acid–Agarose aqueous suspension

Sigma-Aldrich

A8312

m-Aminophenylboronic acid–Agarose

Agarose, low gelling temperature BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture

Sigma-Aldrich

A9045

Agarose, low gelling temperature

m-Aminophenylboronic acid–Agarose saline suspension

Sigma-Aldrich

A8530

m-Aminophenylboronic acid–Agarose

Agarase from Pseudomonas atlantica lyophilized powder, ≥5,000 units/mg protein (Lowry)

Sigma-Aldrich

A6306

Agarase from Pseudomonas atlantica

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

storage temp.

2-8°C

form

aqueous suspension

form

powder

form

saline suspension

form

lyophilized powder

matrix activation

epichlorohydrin

matrix activation

-

matrix activation

epoxy

matrix activation

-

matrix

6% beaded agarose

matrix

-

matrix

cross-linked 6% beaded agarose

matrix

-

matrix attachment

through amino to carboxyls of EDTA

matrix attachment

-

matrix attachment

amino

matrix attachment

-

matrix spacer

9 atoms

matrix spacer

-

matrix spacer

12 atoms

matrix spacer

-

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000280684
0000280684
0000269923
SLCQ0049
SLCL5912

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Related Content

T A Myöhänen et al.
The Biochemical journal, 197(3), 683-688 (1981-09-01)
The synthesis of 3-nitro-4-(6-aminohexylamido)phenylboronic acid is described. The properties of two novel forms of immobilized phenylboronate agarose adsorbents [m-aminophenylboronic acid-Matrex Gel and 3-nitro-4-(6-aminohexylamido)phenylboronic acid-Sepharose CL-6B] were investigated. Both gels bind and selectively retard the glycoprotein alpha-glucosidase from yeast. The retardation
RUNX poly (ADP-ribosyl) ation and BLM interaction facilitate the Fanconi anemia pathway of DNA repair
Tay L S, et al.
Testing, 24(7), 1747-1755 (2018)
Monika Kijewska et al.
Molecules (Basel, Switzerland), 25(3) (2020-02-14)
We report herein a novel ChemMatrix® Rink resin functionalised with two phenylboronate (PhB) moieties linked on the N-α and N-ε amino functions of a lysine residue to specifically capture deoxyfructosylated peptides, compared to differently glycosylated peptides in complex mixtures. The
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Global Trade Item Number

SKUGTIN
A8312-5ML04061833391426

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