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B9754

BIS-TRIS

≥98.0% (titration)

BIS-TRIS

Synonym(s):

2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane

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25 g

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$71.30
100 g

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About This Item

Empirical Formula (Hill Notation):
C8H19NO5
CAS Number:
6976-37-0
Molecular Weight:
209.24
UNSPSC Code:
12161700
NACRES:
NA.25
PubChem Substance ID:
24892155
EC Number:
230-237-7
Beilstein/REAXYS Number:
2205275
MDL number:
MFCD00002853

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Quality Level

400

assay

≥98.0% (titration)

form

crystalline powder

storage condition

dry at room temperature

technique(s)

UV/Vis spectroscopy: suitable, electrophoresis: suitable

color

white

useful pH range

5.8-7.2

pKa (25 °C)

6.5

solubility

H2O: 500 mg/mL, clear, colorless to very faintly yellow

suitability

suitable for Western blot, suitable for column chromatography of proteins

application(s)

advanced drug delivery
diagnostic assay manufacturing
general analytical
microbiology

SMILES string

OCCN(CCO)C(CO)(CO)CO

InChI

1S/C8H19NO5/c10-3-1-9(2-4-11)8(5-12,6-13)7-14/h10-14H,1-7H2

InChI key

OWMVSZAMULFTJU-UHFFFAOYSA-N

General description

Bis-tris methane, also known as BIS-TRIS or BTM, is a zwitterionic buffering agent widely employed in biochemical and biological research. It functions effectively in aqueous solutions within a pH range of 5.8 to 7.2 and demonstrates minimal shifts in its dissociation constant. Further, it can be used as a substitute for the highly toxic buffer cacodylate and is also recognized for its ability to form complexes with different metals.

Application

Bis-Tris has been used :
  • as a buffer for Electrophoresis[1]
  • as a component of transfer buffer for Western Blotting[2]

Features and Benefits

  • Suitable for Biological and Biochemical Research
  • Effective Buffering from pH 5.8-7.2 (25 °C) with a pKa of 6.5 (25 °C)

Other Notes

For additional information on our range of Biochemicals, please complete this form.

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This Item
B442939133514879
BIS-TRIS ≥98.0% (titration)

Sigma-Aldrich

B9754

BIS-TRIS

BIS-TRIS BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, ≥98.0%

Sigma-Aldrich

B4429

BIS-TRIS

BIS-TRIS ULTROL® Grade, ≥99% dry basis (titration), suitable where a calcium phosphate-DNA complex formation is desirable

Millipore

391335

BIS-TRIS

BIS-TRIS BioUltra, ≥99.0% (NT)

Sigma-Aldrich

14879

BIS-TRIS

assay

≥98.0% (titration)

assay

≥98.0%

assay

≥99% dry basis (titration)

assay

≥99.0% (NT)

technique(s)

UV/Vis spectroscopy: suitable, electrophoresis: suitable

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

Quality Level

400

Quality Level

400

Quality Level

100

Quality Level

200

form

crystalline powder

form

crystalline powder

form

solid

form

powder

application(s)

advanced drug delivery
diagnostic assay manufacturing
general analytical
microbiology

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

-

solubility

H2O: 500 mg/mL, clear, colorless to very faintly yellow

solubility

H2O: 500 mg/mL, clear, colorless to very faintly yellow

solubility

water: soluble

solubility

H2O: 1 M at 20 °C, clear, colorless

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pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000560021
0000552411
0000552412
0000549752
0000549754

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Protocols

Enzymatic Activity of Glucose-6-Phosphatase [EC 3.1.3.9]

To measure glucose-6-phosphatase activity, the Taussky-Shorr method is used. This method is a spectrophotometric stop-rate determination assay that is measured at 660 nm.

Related Content

Product Information Sheet - B9754

Product Information Sheet

Raman Kumar et al.
STAR protocols, 3(4), 101693-101693 (2022-09-20)
Immunoprecipitation (IP) of endogenously expressed proteins is one of the most biologically relevant techniques to identify protein-protein interactions. We describe an adaptable IP protocol reliant on a specific antibody to the target protein. We detail a quantitative proteomics workflow for
Elizabeth M MacDonald et al.
Disease models & mechanisms, 7(4), 471-481 (2014-02-08)
The purpose of our study was to compare two acquired muscle atrophies and the use of myostatin inhibition for their treatment. Myostatin naturally inhibits skeletal muscle growth by binding to ActRIIB, a receptor on the cell surface of myofibers. Because
Lesley Cheng et al.
Kidney international, 86(2), 433-444 (2013-12-20)
Micro RNAs (miRNAs) have been shown to circulate in biological fluids and are enclosed in vesicles such as exosomes; they are present in urine and represent a noninvasive methodology to detect biomarkers for diagnostic testing. The low abundance of RNA
View All Related Papers

Global Trade Item Number

SKUGTIN
B9754-500G04061833443965
B9754-1KG04061833443941
B9754-25KG04061832546629
B9754-5KG04061833443972
B9754-100G04061833443934
B9754-25G04061833443958
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Questions

1–2 of 2 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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