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C-12909

Human CD14+ Monocytes (hMoCD14+-PB)

Isolated from peripheral blood, single donor, 10 million cryopreserved cells

Synonym(s):

hMoCD14+-PB cells

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1 ea
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$1,290.00

About This Item

NACRES:
NA.81
UNSPSC Code:
41106514
Biological source:
human peripheral blood
Morphology:
(monocytes)

$1,290.00

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biological source

human peripheral blood

packaging

pkg of 10,000,000 cells

morphology

(monocytes)

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

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This Item
C-12907C-14110C-12921
Human CD14+ Monocytes (hMoCD14+-PB) Isolated from peripheral blood, single donor, 10 million cryopreserved cells

Sigma-Aldrich

C-12909

Human CD14+ Monocytes (hMoCD14+-PB)

Human Mononuclear Cells (hMNC) Isolated from peripheral blood, single donor, ultra-pure, 25 million cryopreserved cells

Sigma-Aldrich

C-12907

Human Mononuclear Cells (hMNC)

Human CD14+ Monocytes (hMoCD14+-PB) Isolated from peripheral blood, single donor, 1 million cells per pellet

Sigma-Aldrich

C-14110

Human CD14+ Monocytes (hMoCD14+-PB)

Human CD34+ Progenitor Cells (hCD34+-CB) Isolated from cord blood, single donor, 100,000 cryopreserved cells

Sigma-Aldrich

C-12921

Human CD34+ Progenitor Cells (hCD34+-CB)

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

biological source

human peripheral blood

biological source

human peripheral blood

biological source

human peripheral blood

biological source

human umbilical cord blood

morphology

(monocytes)

morphology

(mononuclear)

morphology

(monocyte)

morphology

(progenitor)

packaging

pkg of 10,000,000 cells

packaging

pkg of 25,000,000 cells

packaging

pkg of 1,000,000 cells

packaging

pkg of 100,000 cells

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−196°C

storage temp.

−196°C

storage temp.

−20°C

storage temp.

−196°C

General description

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Application

Monocytes (Mo) are immature phagocytic cells circulating in the blood. Acting as antigen-presenting immune cells, they can phagocytize and degrade microbes and particulate matter. Monocytes can differentiate into several different cell types. The most common applications for Monocytes are the in vitro differentiation into monocyte-derived dendritic cells (moDC), macrophages, and osteoclasts as well as their use as phagocytic immune cells in immunology and infection biology.CD14 belongs to the family of LPS receptor antigens and is strongly expressed on the majority of Monocytes. Thus, binding of immunomagnetic particles to this antigen is employed for purification of PromoCell CD14+-Monocytes (hMoCD14+-PB) from Mononuclear Cells (MNC). Human CD14+ Monocytes are isolated from fresh peripheral blood. After isolation of the ultra-pure mononuclear cell fraction by proprietary methods, the CD14+ Monocytes are isolated by positive selection with >95% purity.

Biochem/physiol Actions

Blood

Preparation Note

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Analysis Note

Rigid quality control tests are performed for each lot of Monocytes. They are routinely characterized by flow cytometry analyzing the viability, cell size and purity. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Other Notes

Recommended Plating Density: 1 x 106 cells per mL
Recommended Primary Cell Culture Media:Link

Disclaimer

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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