This product is full-length human cytochrome b5 and includes the transmembrane domain. This product is supplied as a buffered solution and is not incorporated into a membrane bilayer. Proper folding and function within a membrane bilayer have not been specifically validated.
C1427
Cytochrome b5 human
recombinant, expressed in E. coli (histidine-tagged), ≥90% (SDS-PAGE)
Synonym(s):
Cytochrome b5
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Size/SKU | Availability | Price |
|---|---|---|
0.1 mg | Check Cart for Availability | $660.00 |
About This Item
Form:
solution
Assay:
≥90% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in E. coli (histidine-tagged)
Mol wt:
16 kDa
biological source
human
Quality Segment
recombinant
expressed in E. coli (histidine-tagged)
assay
≥90% (SDS-PAGE)
form
solution
mol wt
16 kDa
technique(s)
inhibition assay: suitable
suitability
suitable for molecular biology
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... CYB5A(1528)
General description
Cytochrome b5 (CYB5A) gene codes for cytochrome b5 protein. It is located on human chromosome 18. It is co-expressed in the Leydig cells of the testis and theca cells of the ovary.
Biochem/physiol Actions
Cytochrome b5 is a membrane-bound heme-containing protein that enhances the catalytic efficiency of some P450 isoforms. Its mechanism of action is unknown.
Membrane-bound heme-containing protein; enhances catalytic efficiency of some P450 isoforms.
Cytochrome b5 (CYB5A) mediates NADH-dependent reduction of methemoglobin (erythrocytes), electron transfer to fatty-acid desaturases (mainly liver) and steroid and xenobiotics metabolism by cytochrome P450 enzymes. It is essential for androstenedione synthesis. CYB5A helps in the efficient synthesis of gonadal androgen.
Physical form
Solution in 20 mM phosphate buffer, pH 7.25, with 0.1 mM EDTA
Analysis Note
Heme content measured using difference spectroscopy. An extinction coefficient of 100 mM-1 for the absorbance difference between 490 nm and 424 nm is used to calculate the concentration of cytochrome b5.
1 of 1
This Item | |||
|---|---|---|---|
| description recombinant, expressed in E. coli (histidine-tagged), ≥90% (SDS-PAGE) | description Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution | description Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution | description targeting human CYB5A |
| technique(s) inhibition assay: suitable | technique(s) immunohistochemistry: 1:200-1:500, immunofluorescence: 0.25-2 μg/mL | technique(s) immunofluorescence: 0.25-2 μg/mL, immunoblotting: 0.04-0.4 μg/mL, immunohistochemistry: 1:50-1:200 | technique(s) - |
| recombinant expressed in E. coli (histidine-tagged) | recombinant - | recombinant - | recombinant - |
| biological source human | biological source rabbit | biological source rabbit | biological source - |
| assay ≥90% (SDS-PAGE) | assay - | assay - | assay - |
| suitability suitable for molecular biology | suitability - | suitability - | suitability - |
| mol wt 16 kDa | mol wt - | mol wt - | mol wt - |
Storage Class
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
-
Is this a properly folded functional protein in a membrane bilayer environment? Has this product been tested to work in electron transfer reactions in in vitro enzyme assays?
1 answer-
Helpful?
-
-
does this recombinant version of the protein still contain its membrane bound domain?
1 answer-
This recombinant protein is full-length and includes the transmembrane domain.
Helpful?
-