This recombinant protein is full-length and includes the transmembrane domain.
C1427
Cytochrome b5 human
recombinant, expressed in E. coli (histidine-tagged), ≥90% (SDS-PAGE)
Synonym(s):
Cytochrome b5
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| Size/SKU | Availability | Price |
|---|---|---|
0.1 mg | Check Cart for Availability | $637.00 |
About This Item
Form:
solution
Assay:
≥90% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in E. coli (histidine-tagged)
Mol wt:
16 kDa
biological source
human
Quality Segment
recombinant
expressed in E. coli (histidine-tagged)
assay
≥90% (SDS-PAGE)
form
solution
mol wt
16 kDa
technique(s)
inhibition assay: suitable
suitability
suitable for molecular biology
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... CYB5A(1528)
General description
Cytochrome b5 (CYB5A) gene codes for cytochrome b5 protein. It is located on human chromosome 18. It is co-expressed in the Leydig cells of the testis and theca cells of the ovary.
Biochem/physiol Actions
Cytochrome b5 is a membrane-bound heme-containing protein that enhances the catalytic efficiency of some P450 isoforms. Its mechanism of action is unknown.
Membrane-bound heme-containing protein; enhances catalytic efficiency of some P450 isoforms.
Cytochrome b5 (CYB5A) mediates NADH-dependent reduction of methemoglobin (erythrocytes), electron transfer to fatty-acid desaturases (mainly liver) and steroid and xenobiotics metabolism by cytochrome P450 enzymes. It is essential for androstenedione synthesis. CYB5A helps in the efficient synthesis of gonadal androgen.
Physical form
Solution in 20 mM phosphate buffer, pH 7.25, with 0.1 mM EDTA
Analysis Note
Heme content measured using difference spectroscopy. An extinction coefficient of 100 mM-1 for the absorbance difference between 490 nm and 424 nm is used to calculate the concentration of cytochrome b5.
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This Item | |||
|---|---|---|---|
| description recombinant, expressed in E. coli (histidine-tagged), ≥90% (SDS-PAGE) | description ≥95% (SDS-PAGE), lyophilized powder | description recombinant, expressed in E. coli, ≥80% (SDS-PAGE) | description recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE) |
| technique(s) inhibition assay: suitable | technique(s) cell culture | mammalian: suitable, mass spectrometry (MS): suitable, western blot (chemiluminescent): suitable | technique(s) electrophoretic mobility shift assay: suitable | technique(s) activity assay: suitable, inhibition assay: suitable |
| recombinant expressed in E. coli (histidine-tagged) | recombinant - | recombinant expressed in E. coli | recombinant expressed in baculovirus infected Sf9 cells |
| biological source human | biological source human heart | biological source human | biological source human |
| assay ≥90% (SDS-PAGE) | assay ≥95% (SDS-PAGE) | assay ≥80% (SDS-PAGE) | assay ≥70% (SDS-PAGE) |
| suitability suitable for molecular biology | suitability - | suitability - | suitability - |
| mol wt 16 kDa | mol wt 11 kDa by SDS-PAGE | mol wt ~39.1 kDa | mol wt 62 kDa |
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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does this recombinant version of the protein still contain its membrane bound domain?
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