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C5723

Anti-Cytochrome c antibody produced in sheep

whole antiserum

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0.2 mL
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$659.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, IP, WB
Citations:
6

$659.00


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biological source

sheep

Quality Segment

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

mol wt

antigen 14.4 kDa

species reactivity

human, rabbit, canine, rat

technique(s)

immunoprecipitation (IP): suitable, indirect immunofluorescence: suitable, western blot: 1:5,000 using MCF-7, Rat-1, MDCK or Jurkat cell extracts

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CYCS(54205)
rat ... Cycs(25309)

General description

Cytochrome c is an electron transport protein released from mitochondria as an early committed event in apoptosis. Cytochrome c and dATP are cofactors for the mammalian apoptosome, which is composed of Apaf-1, Bcl-2, and procaspase 9. When caspase 9 is activated, activation of other caspases follow including the death protease caspase 3. The release of cytochrome c is inhibited by the presence of Bcl-2 on these organelles preventing the initiation of apoptosis. In cells induced by several apoptotic agents (such as UV irradiation, staurosporine, and overexpression of Bax), caspase inhibitors do not prevent cytochrome c release.

Immunogen

rabbit cytochrome c

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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description

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conjugate

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biological source

sheep

biological source

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biological source

sheep

biological source

rabbit

antibody form

whole antiserum

antibody form

affinity isolated antibody

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affinity purified immunoglobulin

antibody form

affinity isolated antibody

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

100

mol wt

antigen 14.4 kDa

mol wt

antigen ~200 kDa

mol wt

-

mol wt

antigen 11 kDa

technique(s)

immunoprecipitation (IP): suitable, western blot: 1:5,000 using MCF-7, Rat-1, MDCK or Jurkat cell extracts, indirect immunofluorescence: suitable

technique(s)

indirect immunofluorescence: 1:100 using cultured rat NRK cells, microarray: suitable, western blot: 1:1,000 using whole cell extracts of cultured dog MDCK kidney cells and cultured human Jurkat cells

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

ELISA: 1:10000, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000


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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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