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Anti-Chk1 antibody, Mouse monoclonal

Name: Anti-Chk1 antibody, Mouse monoclonal
Brand: SIGMA
Price: 481.1 USD
Availability: InStock

~2 mg/mL, clone DCS-310, purified from hybridoma cell culture

Synonym(s):

Anti-CHK1

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Size/SKU	Availability	Price
0.2 mL	Available to ship TODAYfromMILWAUKEE	$566.00 $481.10

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD02262709

Conjugate:

unconjugated

Clone:

DCS-310, monoclonal

Application:

ARR, IHC (p), IP, WB

Citations:

$481.10

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

DCS-310, monoclonal

form

buffered aqueous solution

mol wt

antigen 54 kDa

species reactivity

human

concentration

~2 mg/mL

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, microarray: suitable, western blot: 1-2 μg/mL using whole extract of cultured 293T (human embryonal kidney) cells

isotype

IgG2b

UniProt accession no.

O14757

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CHEK1(1111)

Description

General description

Checkpoint kinase 1 (Chk1) protein is a serine/threonine-protein kinase with a molar mass of 54 kDa. Monoclonal Anti-Chk1 (mouse IgG2b isotype) is derived from the DCS-310 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a recombinant human Chk1.

Immunogen

recombinant human Chk1.

Application

Monoclonal Anti-Chk1 antibody produced in mouse has been used in:

immunoprecipitation
immunohistochemistry
immunoblotting

Monoclonal Anti-Chk1 antibody produced in mouse is suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections), immunoprecipitation, microarray and western blot at a working concentration of 1-2 μg/mL using whole extract of cultured 293T (human embryonal kidney) cells. It was used for immunohistochemistry in a study to investigate the specific role of Chk1 phosphorylations in cell survival and checkpoint activation. It was used for immunohistochemistry at a working dilution of 1:4000 in a study to explore Chk1 knockdown as a novel therapeutic approach to arrest cell-cycle progression in MM cells, thus increasing the rate of cell death. It was used for the immunoprecipitation and detection of total Chk1 by western blot to study a possible regulatory relationship between Chk1 and CK1, a member of the casein kinase 1 family.

Biochem/physiol Actions

Checkpoint kinase 1 (Chk1) protein acts as a cell cycle checkpoint regulator.

The protein propagates signals from damaged or unreplicated DNA. It is expressed and active only in S-G2 phases of the cell cycle. In response to DNA damage, it phosphorylates CDC25C at Ser 216, thus preventing activation of the Cdc2-cyclin B complex and mitotic entry, blocking cell cycle progression. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Chk1 can phosphorylate Wee1, a negative regulator of G2 to M transition.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	C7855	SAB4500211	D4941
Sigma-Aldrich C9358 Anti-Chk1 antibody, Mouse monoclonal	Sigma-Aldrich C7855 Anti-Cdh1 antibody,Mouse monoclonal	Sigma-Aldrich SAB4500211 Anti-CHK1 antibody produced in rabbit	Sigma-Aldrich D4941 Anti-phospho-DAP-Kinase (pSer³⁰⁸) antibody, Mouse monoclonal
description ~2 mg/mL, clone DCS-310, purified from hybridoma cell culture	description clone DCS-266, purified from hybridoma cell culture	description affinity isolated antibody	description clone DKPS308, purified from hybridoma cell culture
clone DCS-310, monoclonal	clone DCS-266, monoclonal	clone polyclonal	clone DKPS308, monoclonal
antibody form purified from hybridoma cell culture	antibody form purified from hybridoma cell culture	antibody form affinity isolated antibody	antibody form purified from hybridoma cell culture
Gene Information human ... CHEK1(1111)	Gene Information human ... CDH1(999)	Gene Information human ... CHEK1(1111)	Gene Information human ... DAPK1(1612)
species reactivity human	species reactivity human	species reactivity mouse, rat, human	species reactivity human
biological source mouse	biological source mouse	biological source rabbit	biological source mouse
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c