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D1152

Dulbecco′s Modified Eagle′s Medium - high glucose

HEPES Modification, With 4500 mg/L glucose, L-glutamine, and 25 mM HEPES, without sodium bicarbonate and pyruvate, powder, suitable for cell culture

Synonym(s):

DME, DMEM

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10 L

Available to ship TODAYfromMILWAUKEE

$93.00
10 x 1 L

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$99.80
50 L

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$332.00

About This Item

NACRES:
NA.75
UNSPSC Code:
12352207

$93.00

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form

powder

technique(s)

cell culture | mammalian: suitable

components

sodium pyruvate: no
NaHCO3: no
phenol red: yes
HEPES: 25 mM
L-glutamine: yes
glucose: high

shipped in

ambient

storage temp.

2-8°C

Application

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Preparation Note

Formulated to contain 17.3 grams of powder per liter of medium.
Supplement with 3.7 g/L sodium bicarbonate.

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D7777D5648D6171
Sigma-Aldrich

Sigma-Aldrich

D1152

Dulbecco′s Modified Eagle′s Medium - high glucose

Sigma-Aldrich

Sigma-Aldrich

D7777

Dulbecco′s Modified Eagle′s Medium - high glucose

Dulbecco’s Modified Eagle’s Medium - high glucose With 4500 mg/L glucose and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Sigma-Aldrich

D5648

Dulbecco′s Modified Eagle′s Medium - high glucose

Dulbecco’s Modified Eagle’s Medium - high glucose HEPES modification, With 4500 mg/L glucose, 25 mM HEPES, and sodium bicarbonate, without L-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

SAFC

D6171

Dulbecco′s Modified Eagle′s Medium - high glucose

form

powder

form

powder

form

powder

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

sodium pyruvate: no
NaHCO3: no
phenol red: yes
HEPES: 25 mM
L-glutamine: yes
glucose: high

components

glucose: high
sodium pyruvate: yes
NaHCO3: no
HEPES: no
phenol red: yes
L-glutamine: yes

components

phenol red: yes
L-glutamine: yes
NaHCO3: no
HEPES: no
glucose: high
sodium pyruvate: no

components

sodium pyruvate: no
HEPES: 25 mM
glucose: high
NaHCO3: yes
L-glutamine: no
phenol red: yes

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

classic media, classical media, FBS, glucose enriched

classic media, classical media, FBS, glucose enriched

classic media, classical media, FBS, glucose enriched

classic media, classical media, serum supplement, FBS, HEPES, high glucose, pyridoxine

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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000534309
0000534307
0000534309
0000534310
0000519691

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Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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Global Trade Item Number

SKUGTIN
D1152-50K04061834180180
D1152-1L04061837468025
D1152-10L04061838259226
D1152-50L04061833558775
D1152-10X1L04061838259233

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