Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
D5637
3,3′-Diaminobenzidine tetrahydrochloride hydrate
≥96%
Synonym(s):
(1,1′-Biphenyl)-3,3′,4,4′-tetramine tetrahydrochloride dihydrate
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 g | Please contact Customer Service for Availability | €50.30 | |
| 5 g | Please contact Customer Service for Availability | €166.00 | |
| 10 g | Please contact Customer Service for Availability | €307.00 | |
| 25 g | Please contact Customer Service for Availability | €661.00 | |
| 50 g | Please contact Customer Service for Availability | €992.00 |
About This Item
Linear Formula:
C12H14N4 · 4HCl · xH2O
CAS Number:
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
231-018-9
MDL number:
Quality Level
assay
≥96%
form
powder, crystals or chunks
technique(s)
titration: 96-104% (with NaOH)
color
white to light brown, and Faint Red and Faint Grey to Light Grey
mp
280 °C
solubility
H2O: 50 mg/mL
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
−20°C
SMILES string
O.Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2
InChI
1S/C12H14N4.4ClH.H2O/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;;/h1-6H,13-16H2;4*1H;1H2
InChI key
DXWSCXIZIHILNP-UHFFFAOYSA-N
General description
3,3′-Diaminobenzidine tetrahydrochloride hydrate is a chromogen commonly used in immunohistochemistry and other staining techniques to visualize the presence of antigens or antibodies in biological samples. DAB HCl is designed as an immunohistology stain to visualize peroxidase activity in cells. DAB oxidation can be measured using a spectrophotometer.
Application
3,3′-Diaminobenzidine tetrahydrochloride hydrate is suitable for immunohistology staining procedures, western blotting, and FISH (fluorescence in situ hybridization). DAB is used in studying epithelial cell apoptosis in the event of early cataracts, and immunohistochemistry protocols of autosomal dominant polycystic kidney disease (PKD).
Biochem/physiol Actions
In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidized DAB forms an insoluble brown-colored complex.
1 of 1
This Item | |||
|---|---|---|---|
| technique(s) titration: 96-104% (with NaOH) | technique(s) - | technique(s) - | technique(s) UV/Vis spectroscopy: suitable |
| color white to light brown, and Faint Red and Faint Grey to Light Grey | color off-white to faint red, to beige | color - | color - |
| solubility H2O: 50 mg/mL | solubility water: 50 mg/mL, clear to slightly hazy | solubility acetic acid: water (1:2): 20 mg/mL, clear to slightly hazy | solubility - |
| form powder, crystals or chunks | form powder | form powder | form - |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) - | application(s) - |
| assay ≥96% | assay ≥96% (TLC) | assay ≥98% | assay ≥97.5% |
Still not finding the right product?
Explore all of our products under 3,3′-Diaminobenzidine tetrahydrochloride hydrate
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
868272-85-9
CAS No.
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Articles
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
Related Content
Instructions
Fatima Solange Pasini et al.
Journal of gastroenterology, 49(11), 1453-1466 (2013-11-13)
The TNM Classification of Malignant Tumours (TNM) staging system is the primary means of determining a prognosis for gastric adenocarcinoma (GC). However, tumor behavior in the individual patient is unpredictable and in spite of treatment advances, a classification of 'advanced
Franc Llorens et al.
Nature communications, 11(1), 619-619 (2020-02-01)
The clinical diagnosis of vascular dementia (VaD) is based on imaging criteria, and specific biochemical markers are not available. Here, we investigated the potential of cerebrospinal fluid (CSF) lipocalin 2 (LCN2), a secreted glycoprotein that has been suggested as mediating
Nicole M Derks et al.
Neuroscience research, 66(1), 117-123 (2009-10-17)
Women have higher vulnerability to stress and stress-induced diseases than men. Estrogen may be involved in the control of sex-dependent stress adaptation via estrogen receptors alpha and beta (ERalpha/beta). Urocortin 1 (Ucn1) in the npEW plays an important role in
Global Trade Item Number
| SKU | GTIN |
|---|---|
| D5637-50G | 04061835347506 |
| D5637-1G | 04061835347483 |
| D5637-25G | 04061835347490 |
| D5637-5G | 04061835347513 |
| D5637-10G | 04061835347476 |
-
What is the Department of Transportation shipping information for this product?
1 answer-
Helpful?
-
-
Do you have a staining protocol for Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB)?
1 answer-
Dissolve 10 mg in 15 mL of Tris buffered saline, pH 7.6 (Tris-buffered saline tablet, Sigma Product No. T5030 may be used). Add 12 uL of fresh 30% hydrogen peroxide (Sigma Product No. H1009) prior to use. Solution may be filtered through a 0.2 micron filter immediately prior to use if necessary. DAB and hydrogen peroxide concentrations may be adjusted to suit individual applications. The stain can be enhanced with Ni. Make up 0.3% (w/v) NiCl2 in water. Add 1 mL of the NiCl2 stock to 9 mL DAB solution.
Helpful?
-
-
What is the solution stability of Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB)?
1 answer-
In our experience, solutions of DAB should be made immediately prior to use for best results. If and only if necessary, solutions can be kept in the dark and refrigerated for overnight storage. Based on our Immunology Department’s experience, storing the solution overnight did not give good reproducibility. However, the literature indicates that it is possible to store solutions of DAB at -20°C. DAB dissolved in Tris buffered saline (pH 7.6) at 0.6 mg/ml was reported to be stable for several weeks at 4°C.
Helpful?
-
-
How is Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB), shipped and stored?
1 answer-
The product is shipped at ambient temperature. However, it is stored at -20 degrees C for long term storage.
Helpful?
-
-
Can the solutions of Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB), be filtered?
1 answer-
DAB solution may be filtered through a 0.2 micron filter immediately prior to use.
Helpful?
-
-
What is Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB), soluble in?
1 answer-
DAB HCl is soluble in water at 50 mg/ml yielding a clear to slightly hazy solution. For consistency, it is important to allow DAB to come to room temperature before making solutions. Water quality is extremely important, so only high quality deionized or distilled water should be used to make DAB solutions.
Helpful?
-
-
What is the shelf life of Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB)?
1 answer-
This product has a shelf life of 3 years.
Helpful?
-