biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
SPE-7, monoclonal
form
buffered aqueous solution
concentration
~1 mg/mL
technique(s)
indirect ELISA: 0.05-0.1 μg/mL using using 0.5 μg/well DNP-BSA as the coating antigen and 1 μg/mL Monoclonal Anti-Mouse IgE, Peroxidase conjugate as the detector antibody.
isotype
IgE
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
IgE基因在人类染色体14上编码。IgE是循环血中含量最少的抗体,但对于引发过敏原导致的超敏反应至关重要。
Immunogen
Application
二硝基苯单克隆抗体特异性小鼠IgE已被用于通过酶标仪和荧光分光光度计检测大鼠肥大细胞的脱颗粒反应。
蛋白质免疫印迹分析(1篇论文)
Biochem/physiol Actions
Physical form
Preparation Note
Disclaimer
1 of 1
本產品 | |||
|---|---|---|---|
| description IgE isotype, ~1 mg/mL, clone SPE-7, affinity purified immunoglobulin, buffered aqueous solution | description clone SPE-7, purified immunoglobulin, buffered aqueous glycerol solution | description clone GG-7, purified from hybridoma cell culture | description clone HP-6025, purified from hybridoma cell culture |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| clone SPE-7, monoclonal | clone SPE-7, monoclonal | clone GG-7, monoclonal | clone HP-6025, monoclonal |
| conjugate unconjugated | conjugate alkaline phosphatase conjugate | conjugate - | conjugate - |
| antibody form affinity purified immunoglobulin | antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form purified immunoglobulin |
| technique(s) indirect ELISA: 0.05-0.1 μg/mL using using 0.5 μg/well DNP-BSA as the coating antigen and 1 μg/mL Monoclonal Anti-Mouse IgE, Peroxidase conjugate as the detector antibody. | technique(s) direct ELISA: 1:25,000 using DNP-BSA, dot blot: 1:180,000 (chemiluminescence) | technique(s) ELISA: suitable, immunofluorescence: suitable, indirect ELISA: 0.25-0.5 μg/mL using 5μg/mL human IgG for coating. | technique(s) immunoblotting: suitable, indirect ELISA: 0.15-0.3 μg/mL using 1 μg/ml human IgG4 myeloma proteins for coating. |
| isotype IgE | isotype IgE | isotype IgG1 | isotype IgG1 |
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存儲類別/等級
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable



