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E9762

Endoglycosidase F1

Name: Endoglycosidase F1
Brand: SIGMA

Cleaves within N-linked glycans, suitable for Mass Spectrometry, from Elizabethkingia miricola, recombinant, expressed in E. coli

Synonyme(s) :

Endoglycosidase F1, Endoglycosidase F1 from Elizabethkingia meningoseptica

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A propos de cet article

UNSPSC Code:

12352204

NACRES:

NA.32

Numéro CE :

3.2.1.96 (BRENDA, IUBMB)

MDL number:

MFCD00146843

Specific activity:

≥16 U/mg

Recombinant:

expressed in E. coli

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Propriétés

Nom du produit

Endoglycosidase F1, recombinant, expressed in E. coli, ≥16 U/mg, buffered aqueous solution

recombinant

expressed in E. coli

Quality Segment

200

conjugate

(N-linked)

form

buffered aqueous solution

specific activity

≥16 U/mg

mol wt

32 kDa

shipped in

wet ice

storage temp.

2-8°C

Description

General description

Endoglycosidase F1 from Elizabethkingia miricola is a glycan specific enzyme.

Application

Cleaves asparagine-linked or free oligomannose and hybrid, but not complex, oligosaccharides.

Endoglycosidase F1 from Elizabethkingia miricola has been used to remove binding of human hemochromatosis protein (HFE) to cation independent mannose-6-phosphate receptor (CI-MPR) and to glycosylate fucosylated N-glycopeptides.

Biochem/physiol Actions

Endoglycosidase F1 from Elizabethkingia miricola plays an important role in cleavage of glycan structures from the protein by cleaving between the two N-acetylglucosamine residues of the chitobiose core. It mediates high mannose and hybrid oligosaccharides cleavage.

Packaging

Supplied with 5× Reaction Buffer, 250 mM NaH₂PO₄, pH 5.5.

Physical form

Aseptically filled solution in 20 mM Tris-HCl pH 7.5

Other Notes

One unit will release N-linked oligosaccharides from 1 μmole of denatured Ribonuclease B in 1 minute at 37 °C, pH 5.5.

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Cet article	E0639	E2264	324725
Sigma-Aldrich E9762 Endoglycosidase F1	Sigma-Aldrich E0639 Endoglycosidase F2 from Elizabethkingia miricola	Sigma-Aldrich E2264 Endoglycosidase F3 from Elizabethkingia miricola	Sigma-Aldrich 324725 Endoglycosidase F1, Elizabethkingia meningosepticum, Recombinant, E. coli
description recombinant, expressed in E. coli, ≥16 U/mg, buffered aqueous solution	description recombinant, expressed in E. coli, 20 U/mg	description recombinant, expressed in E. coli, 30 U/mg	description Endoglycosidase F1, Elizabethkingia meningosepticum, Recombinant, E. coli cleaves asparagine-linked or free oligomannose and hybrid. Suitable for deglycosylation of native proteins.
specific activity ≥16 U/mg	specific activity 20 U/mg	specific activity 30 U/mg	specific activity ≥16 units/mg protein, ≥17 units/mL
recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in E. coli
form buffered aqueous solution	form solution	form solution	form liquid
mol wt 32 kDa	mol wt 32 kDa	mol wt 32 kDa	mol wt -
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in wet ice	shipped in wet ice	shipped in wet ice	shipped in wet ice

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Classe de stockage

10 - Combustible liquids

wgk

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flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

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