EMS0004
SOLu-Trypsin Protease
Ready-to-use stable solution for automation, suitable for mass spectrometry, recombinant, expressed in Pichia pastoris
Synonym(s):
Protein Digestion for Mass Spectrometry, Ready to Use Recombinant Trypsin for Protein Digestion in Mass Spectrometry, Recombinant Trypsin for Protein Digestion in Mass Spectrometry, Trypsin for Protein Digestion, rTrypsin
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 100 μg | Please contact Customer Service for Availability | $125.00 | |
| 4 x 100 μg | Please contact Customer Service for Availability | $466.00 |
About This Item
Product Name
SOLu-Trypsin, recombinant, expressed in Pichia pastoris, Proteomics Grade, liquid
recombinant
expressed in Pichia pastoris
Quality Segment
grade
Proteomics Grade
description
Activity:≥ 10,000 unit/mg protein
form
ready-to-use solution
suitability
suitable for mass spectrometry
UniProt accession no.
shipped in
wet ice
storage temp.
2-8°C
General description
Application
- has been used to digest the gel pieces for mass spectrometry identification of active deubiquitnases (DUBs)[1]
- has been used in trypsin sensitivity assay
- may be used for the digestion of wine proteins
Biochem/physiol Actions
- SOLu-Trypsin (EMS0004) is our exclusive, Advanced Proteomics Grade enzyme that is solution-stable for mass spectrometry.
- Designed to be stable in solution when refrigerated, SOLu-Trypsin can be used immediately without preparation.
- Other forms of trypsin require thawing or reconstitution, and must be discarded if not used immediately.
- SOLu-Trypsin allows excess product to be saved for future use, thus eliminating unnecessary waste and cost.
- It is formulated with a high-purity recombinant trypsin, free of chymotryptic activity, to ensure high fidelity digestion.
Features and Benefits
- Ready to use - no preparation, such as reconstitution or thawing, is required
- Fits seamlessly into established workflow - no need to modify protocols
- Eliminates waste - remains stable in the refrigerator after use so there is no need to discard excess product
- Recombinant, porcine sequence - no chymotryptic activity
- Stable for short-term use at room temperature in an autosampler or on a liquid handling robot
1 of 1
This Item | |||
|---|---|---|---|
| recombinant expressed in Pichia pastoris | recombinant - | recombinant expressed in HEK 293 cells | recombinant - |
| grade Proteomics Grade | grade - | grade - | grade - |
| form ready-to-use solution | form ready-to-use solution | form liquid | form ready-to-use solution |
| suitability suitable for mass spectrometry | suitability - | suitability suitable for mass spectrometry (internal calibrator) | suitability - |
| shipped in wet ice | shipped in wet ice | shipped in dry ice | shipped in - |
| UniProt accession no. | UniProt accession no. - | UniProt accession no. | UniProt accession no. - |
Still not finding the right product?
Explore all of our products under SOLu-Trypsin
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Protocols
An optimized LC-MS/MS based workflow for low artifact tryptic digestion and peptide mapping of monoclonal antibody, adalimumab (Humira) using filter assisted sample preparation (FASP).
Articles
Method development for protein fingerprinting of AAV serotype 5 using both intact mass analysis and peptide mapping, to determine critical quality attributes for gene therapy, utilizing three different columns.
An optimized peptide mapping protocol using NISTmAb as a model monoclonal antibody, shorter incubation times, and improved digestion buffer to demonstrate minimal artificial asparagine deamidation and methionine oxidation.
This application note compares three different column chemistries from the BIOshell™ line of columns with superficially porous particles to evaluate their performance in peptide mapping.
Related Content
Instructions
Global Trade Item Number
| SKU | GTIN |
|---|---|
| EMS0004-4X100UG | 04061822415751 |
| EMS0004-100UG | 04061833253205 |