EMS0004
SOLu-Trypsin Protease
Ready-to-use stable solution for automation, suitable for mass spectrometry, recombinant, expressed in Pichia pastoris
Synonym(s):
Protein Digestion for Mass Spectrometry, Ready to Use Recombinant Trypsin for Protein Digestion in Mass Spectrometry, Recombinant Trypsin for Protein Digestion in Mass Spectrometry, Trypsin for Protein Digestion, rTrypsin
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About This Item
Product Name
SOLu-Trypsin, recombinant, expressed in Pichia pastoris, Proteomics Grade, liquid
recombinant
expressed in Pichia pastoris
Quality Level
grade
Proteomics Grade
description
Activity:≥ 10,000 unit/mg protein
form
ready-to-use solution
suitability
suitable for mass spectrometry
UniProt accession no.
shipped in
wet ice
storage temp.
2-8°C
General description
Application
- has been used to digest the gel pieces for mass spectrometry identification of active deubiquitnases (DUBs)[1]
- has been used in trypsin sensitivity assay
- may be used for the digestion of wine proteins
Biochem/physiol Actions
- SOLu-Trypsin (EMS0004) is our exclusive, Advanced Proteomics Grade enzyme that is solution-stable for mass spectrometry.
- Designed to be stable in solution when refrigerated, SOLu-Trypsin can be used immediately without preparation.
- Other forms of trypsin require thawing or reconstitution, and must be discarded if not used immediately.
- SOLu-Trypsin allows excess product to be saved for future use, thus eliminating unnecessary waste and cost.
- It is formulated with a high-purity recombinant trypsin, free of chymotryptic activity, to ensure high fidelity digestion.
Features and Benefits
- Ready to use - no preparation, such as reconstitution or thawing, is required
- Fits seamlessly into established workflow - no need to modify protocols
- Eliminates waste - remains stable in the refrigerator after use so there is no need to discard excess product
- Recombinant, porcine sequence - no chymotryptic activity
- Stable for short-term use at room temperature in an autosampler or on a liquid handling robot
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This Item | |||
|---|---|---|---|
| recombinant expressed in Pichia pastoris | recombinant expressed in Pichia pastoris | recombinant expressed in HEK 293 cells | recombinant - |
| grade Proteomics Grade | grade Proteomics Grade | grade - | grade - |
| form ready-to-use solution | form ready-to-use solution | form liquid | form ready-to-use solution |
| suitability suitable for mass spectrometry | suitability suitable for mass spectrometry | suitability suitable for mass spectrometry (internal calibrator) | suitability - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. - |
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
An optimized LC-MS/MS based workflow for low artifact tryptic digestion and peptide mapping of monoclonal antibody, adalimumab (Humira) using filter assisted sample preparation (FASP).
Articles
Evaluation of Recombinant, Chemically Treated Trypsin in Proteomics and Protein Characterization Assays
Rapid trypsin digest kit yields reliable results in less than 2 hours for mass spectrometry analysis.
Method development for protein fingerprinting of AAV serotype 5 using both intact mass analysis and peptide mapping, to determine critical quality attributes for gene therapy, utilizing three different columns.
Related Content
Instructions
Global Trade Item Number
| SKU | GTIN |
|---|---|
| EMS0004-100UG | 04061833253205 |
| EMS0004-4X100UG | 04061822415751 |