The stability of this compound in solution has not been evaluated. However, external sources indicate that stock solutions prepared in DMSO are stable for up to 6 months at -20°C when protected from light. Similar compound DCFHDA, product 35845, is stable in organic solvents for up to 6 months at -20°C, when protected from light.
F7378
Fluorescein diacetate
Powder
Synonym(s):
3,6-Diacetoxyfluoran, Di-O-acetylfluorescein
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 5 g | Check Cart for Availability | $94.40 | |
| 10 g | Check Cart for Availability | $177.00 | |
| 25 g | Check Cart for Availability | $363.00 | |
| 100 g | Check Cart for Availability | $1,040.00 |
About This Item
Empirical Formula (Hill Notation):
C24H16O7
CAS Number:
Molecular Weight:
416.38
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
209-877-6
MDL number:
Beilstein/REAXYS Number:
62575
Product Name
Fluorescein diacetate, used as cell viability stain
Quality Level
form
powder
technique(s)
titration: suitable
color
white to faint yellow
mp
200-203 °C (lit.)
solubility
acetone: 25 mg/mL
ε (extinction coefficient)
≥86000 at 488-494 nm in 0.1 M NaOH
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5ccccc45)c1
InChI
1S/C24H16O7/c1-13(25)28-15-7-9-19-21(11-15)30-22-12-16(29-14(2)26)8-10-20(22)24(19)18-6-4-3-5-17(18)23(27)31-24/h3-12H,1-2H3
InChI key
CHADEQDQBURGHL-UHFFFAOYSA-N
General description
Lipase substrate
Fluorescein diacetate (FDA) is a non-fluorescent molecule, which is hydrolysed to fluorescent fluorescein in live cells. Dead cells cannot accumulate or hydrolyse FDA.
Application
Fluorescein diacetate has been used for the differentiation of live cells from dead cells.
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This Item | |||
|---|---|---|---|
| technique(s) titration: suitable | technique(s) - | technique(s) - | technique(s) titration: suitable |
| color white to faint yellow | color - | color white to yellow | color orange to red-brown |
| application(s) diagnostic assay manufacturing | application(s) - | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| form powder | form powder | form powder | form powder |
| solubility acetone: 25 mg/mL | solubility DMSO: soluble | solubility chloroform/ethanol (1:1): 9.80-10.20 mg/mL, clear, colorless to light yellow | solubility - |
| storage temp. room temp | storage temp. 2-8°C | storage temp. −20°C | storage temp. room temp |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| F7378-5G | 04061835347742 |
| F7378-10G | 04061835347728 |
| F7378-25G | 04061835347735 |
| F7378-100G | 04061833619599 |
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How long can you store the FdA F7378 dissolved in acetone (5mg/ml) at -20°C?
1 answer-
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Storage of the product
1 answer-
This product is stored at room temperature.
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After Product F7378, Fluorescein diacetate, has been converted to fluorescein, what are the maximum excitation and emission wavelengths?
1 answer-
The maximum excitation wavelength is 490 nm and the maximum emission wavelength is 526 nm.
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Is Product F7378, Fluorescein diacetate, a fluorescent compound?
1 answer-
No. Only upon hydrolysis of the esters, catalyzed by cellular esterase, the lactone ring opens yielding fluorescein. Only the fluorescein form is fluorescent.
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What solubility information do you have on Product F7378, Fluorescein diacetate?
1 answer-
We have found that it is slightly soluble in water at 0.1%, and in ethanol at 0.3%. It is also soluble in dimethylformamide (DMF).
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What are some common applications for Product F7378, Fluorescein diacetate?
1 answer-
This compound is used extensively for the staining of living cells. It has been used as a viability stain for cultured neurons and plant ways, and in measuring of esterase and cholinesterase activity. See the following references for usage: Fluorometric determination of lipase: Guilbault, G.G. and Kramer, D.N., "Fluorometric Determination of Lipase, Acylase, Alpha- and Gamma- Chymotrypsin and Inhibitors of These Enzymes", Anal. Chem. 36, 409, 1964. ; Anal. Biochem., Vol. 14, 28 (1966);Fluorescent stain for viable rosette-forming cells: J. Immun. Meth., Vol. 5,305 (1974);Fluorogenic substrate detection of viable pathogenic protozoa: Science, Vol. 227, 435 (1985);Reference Standard in antibody studies: Anal. Biochem., Vol. 9, 474 (1964);Cell viability staining: Staining Procedures, G. Clark, 4th ed., p. 93.Guilbault, G.G. and Kramer, D.N., "Fluorometric Determination of Lipase, Acylase, Alpha- and Gamma- Chymotrypsin and Inhibitors of These Enzymes, Anal. Chem. 36, 409, 1964."" "
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How stable are solutions of Product F7378, Fluorescein diacetate, once prepared?
1 answer-
Based on our experience, stock solutions of fluorescein diacetate at 5 mg/mL in acetone or DMSO can be stored at -20 °C. According to one on-line source, solutions at 5 mg/mL in acetone or ethanol can be stored refrigerated.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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