FNCAS9PROT
FnCas9 Protein
from Francisella novicida, recombinant, expressed in E. coli, 1X NLS
Synonym(s):
Francisella Novicida Cas9
Select a Size
| Size/SKU | Availability | Price |
|---|---|---|
50 μg | Estimated to ship onOctober 01, 2026fromMILWAUKEE | $121.00 |
250 μg | Estimated to ship onOctober 05, 2026fromMILWAUKEE | $441.00 $427.77 |
About This Item
recombinant
expressed in E. coli
Quality Segment
assay
≥95% (SDS-PAGE)
form
lyophilized powder
packaging
pkg of 1 kit (4 components)
application(s)
CRISPR
shipped in
wet ice
storage temp.
−20°C
General description
Application
Biochem/physiol Actions
Features and Benefits
- Suitable for plasmid or large genomic DNA fragment cloning
- Results in 4-bp 5′ overhangs.
- Cuts purified plasmid DNA more efficiently than SpCas9
Packaging
pkg of 250 μg (≥ 1500 pmol)
Preparation Note
Other Notes
Enter the 20bp genomic DNA target sequence (5′ to 3′) plus the adjacent 3′ PAM site. Under special instructions, indicate "FnCas9 crRNA"
Click here to order FnCas9 tracrRNA
Check out our other MISSION® Cas9 Proteins at SigmaAldrich.com/CRISPRproteins
- one vial of FnCas9 recombinant protein
- one vial containing 1 mL of 1
× Dilution buffer - one vial containing 1 mL of Nuclease free water with glycerol
- one vial containing 1 mL of 10× Digestion Buffer
Legal Information
1 of 1
This Item | |||
|---|---|---|---|
| description from Francisella novicida, recombinant, expressed in E. coli, 1X NLS | description from Streptococcus pyogenes, recombinant, expressed in E. coli, 1X NLS | description from Streptococcus pyogenes, fused with enhanced GFP, recombinant, expressed in E. coli, 3X NLS | description from Streptococcus pyogenes with mutations conferring enhanced specificity, recombinant, expressed in E. coli, 1X NLS |
| recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in E. coli |
| assay ≥95% (SDS-PAGE) | assay ≥95% (SDS-PAGE) | assay ≥90% (SDS-PAGE) | assay ≥95% (SDS-PAGE) |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in ambient |
| packaging pkg of 1 kit (4 components) | packaging pkg of 1 kit (3 components) | packaging pkg of 1 kit (3 components) | packaging pkg of 1 kit (3 components) |
Kit Components Only
- FnCas9-NLS from Francisella novicida, expressed in Escherichia coli
- Dilution buffer for Cas9 proteins
- Reconstitution solution for Cas9 proteins
- Digestion buffer 10× for FnCas9
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
Storage Class
11 - Combustible Solids
wgk
WGK 3
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.