The concentration and volume of this product varies from lot to lot. The protein content and activity of this enzyme are reported on the lot-specific Certificate of Analysis. The volume may be calculated using this information. For example, the 200 unit package size of a given lot that reports a protein content of 2 mg/mL and an activity of 600 units per milligram protein will have a volume of 167 uL. Please see the link below to review a sample or lot-specific Certificate:
https://www.sigmaaldrich.com/US/en/product/sigma/g5760#product-documentation
G5760
Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides
Type XXIII, ammonium sulfate suspension, 550-1,100 units/mg protein (biuret), ≥2.0 mg/mL Biuret
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Synonym(s):
G-6-P-DH
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| Size/SKU | Availability | Price |
|---|---|---|
200 units | Check Cart for Availability | $78.00 |
1000 units | Available to ship TODAYfromMILWAUKEE | $228.00 |
2000 units | Check Cart for Availability | $393.00 |
10000 units | Available to ship TODAYfromMILWAUKEE | $1,350.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-602-6
MDL number:
Specific activity:
550-1,100 units/mg protein (biuret)
Biological source:
bacterial (Leuconostoc mesenteroides)
Concentration:
≥2.0 mg/mL Biuret
biological source
bacterial (Leuconostoc mesenteroides)
Quality Segment
type
Type XXIII
form
ammonium sulfate suspension
specific activity
550-1,100 units/mg protein (biuret)
mol wt
54 kDa
concentration
≥2.0 mg/mL Biuret
foreign activity
6-Phosphogluconic dehydrogenase, hexokinase, NADH oxidase and NADPH oxidase ≤0.005%, PGI ≤0.01%
storage temp.
2-8°C
General description
Research area: Cell Signaling
Glucose-6-phosphate dehydrogenase (G6PD) is found in the cytoplasm of all cells. The G6PD enzyme is encoded by the Gd gene, located on the long arm of the X chromosome.Glucose-6-phosphate Dehydrogenase (G6PD) from Leuconostoc mesenteroides catalyzes the oxidation of glucose 6-phosphate in the presence of nicotinamide adenine dinucleotide (NAD+) or nicotinamide adenine dinucleotide phosphate (NADP+). It corresponds to a molecular weight of 54 kDa and exists as a homodimer.
Glucose-6-phosphate dehydrogenase (G6PD) is found in the cytoplasm of all cells. The G6PD enzyme is encoded by the Gd gene, located on the long arm of the X chromosome.Glucose-6-phosphate Dehydrogenase (G6PD) from Leuconostoc mesenteroides catalyzes the oxidation of glucose 6-phosphate in the presence of nicotinamide adenine dinucleotide (NAD+) or nicotinamide adenine dinucleotide phosphate (NADP+). It corresponds to a molecular weight of 54 kDa and exists as a homodimer.
Application
Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides has been used:
- as a component of reaction mixture for assaying mannose- and glucose-6-phosphate activity
- as a component of reaction mixture in the nevirapine inhibition studies in human hepatic microsomes
- in the glucose-phosphorylating activity of chloroplast extracts
- as a model to test the effect of seed protein fractions on enzyme protection during dehydration.
- in assays for nicotinamide adenine dinucleotide and tissue pyridine nucleotides.
Biochem/physiol Actions
Glucose-6-phosphate dehydrogenase (G6PD) is a housekeeping enzyme that protects cells from damage caused by reactive oxygen species by supplying substrates that help prevent oxidative harm. G6PD from Leuconostoc mesenteroides catalyzes the oxidation of glucose 6-phosphate in the presence of nicotinamide adenine dinucleotide (NAD+) or nicotinamide adenine dinucleotide phosphate (NADP+) as the first step in the pentose phosphate pathway. Lysine 21 in the Glucose-6-phosphate Dehydrogenase (G6PD) is crucial for binding NAD+. G6PD uses NAD+ or NADP+ based on the catabolic or anabolic metabolic pathway.G6PD deficiency can lead to acute hemolysis, neonatal jaundice, or severe chronic non-spherocytic hemolytic anemia.
Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.
Physical form
Suspension in 2.7 M (NH4)2SO4 solution containing 42 mM Tris and 0.8 mM MgCl2
Other Notes
One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NAD at pH 7.8 at 30 °C.
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This Item | |||
|---|---|---|---|
| description Type XXIII, ammonium sulfate suspension, 550-1,100 units/mg protein (biuret), ≥2.0 mg/mL Biuret | description recombinant, expressed in E. coli, ammonium sulfate suspension, ≥550 units/mg protein (biuret) | description lyophilized powder, >= 550 units/mg protein (biuret) | description from Leuconostoc mesenteroides |
| biological source bacterial (Leuconostoc mesenteroides) | biological source bacterial (Leuconostoc mesenteroides) | biological source bacterial (Leuconostoc mesenteroides) | biological source bacterial (Leuconostoc mesenteroides) |
| specific activity 550-1,100 units/mg protein (biuret) | specific activity ≥550 units/mg protein (biuret) | specific activity >= 550 units/mg protein (biuret) | specific activity ~550 units/mg protein (At 25 °C (650 U/mg at 30 °C) with glucose-6-P and NAD as the substrates.) |
| concentration ≥2.0 mg/mL Biuret | concentration - | concentration - | concentration ≥0.1-1.0 % (w/w) |
| form ammonium sulfate suspension | form ammonium sulfate suspension | form lyophilized powder | form solution, suspension |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| mol wt 54 kDa | mol wt 128 kDa | mol wt 128 kDa | mol wt dimer 110 kDa |
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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