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G8404

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides

recombinant, expressed in E. coli, ammonium sulfate suspension, ≥550 units/mg protein (biuret)

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Synonym(s):

Entner-Doudoroff enzyme, G6PD, G6PDH, NADP glucose 6-phosphate dehydrogenase, G-6-P-DH

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Size/SKUAvailabilityPrice
200 units

Available to ship TODAYfromMILWAUKEE

$78.70
1000 units

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$167.00
2000 units

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$271.00
10000 units

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$920.00

About This Item

CAS Number:
9001-40-5
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
1.1.1.49 (BRENDA, IUBMB)
MDL number:
MFCD00081656
Specific activity:
≥550 units/mg protein (biuret)
Biological source:
bacterial (Leuconostoc mesenteroides)
Recombinant:
expressed in E. coli

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biological source

bacterial (Leuconostoc mesenteroides)

Quality Segment

recombinant

expressed in E. coli

form

ammonium sulfate suspension

specific activity

≥550 units/mg protein (biuret)

mol wt

128 kDa

storage condition

(Tightly closed)

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

foreign activity

NADH oxidase, NADPH oxidase, 6-phosphogluconic dehydrogenase, phosphoglucose isomerase, hexokinase ≤0.01%

storage temp.

2-8°C

General description

Glucose 6-phosphate dehydrogenase (G-6-P-DH) is a key regulatory enzyme in the first step of the pentose phosphate pathway. G-6-P-DH is a glycoprotein with a molecular mass of 128 kDa (gel filtration).G6PD is a dimer and consists of a single active site at each subunit.

Research area: Cell Signaling

Application

Glucose-6-phosphate dehydrogenase has been used:

  • to test ketose reductase activity in developing maize endosperm.
  • to determine the levels of mannose in coronary heart disease patient-derived serum
  • to study its activity on extracellular polymeric substance (EPS) extract to determine cell lysis through sonication
  • to determine the glucose uptake in cultured human muscle satellite cells

Biochem/physiol Actions

G6PD provides all cells with reducing power as nicotinamide adenine dinucleotide phosphate (NADPH) which enables the cells to balance oxidative stress. Mutations in the G6PD gene are associated with X-linked G6PD deficiency, a hereditary genetic defect such as neonatal jaundice and acute hemolytic anemia.
Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.

Physical form

Suspension in 3.2 M (NH4)2SO4 containing 50 mM Tris and 1 mM MgCl2, pH 7.5

Other Notes

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NAD at pH 7.8 at 30 °C.
The volume is lot specific and can be calculated using the data:
For example, lot 0000114274
Units per mg Protein = 846
Mg Protein per mL - 9
Units per mL = 7614
G8404-1000U = approx 132 ul

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This Item
G8529G5760G5885
Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides recombinant, expressed in E. coli, ammonium sulfate suspension, ≥550 units/mg protein (biuret)

Sigma-Aldrich

G8404

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides recombinant, expressed in E. coli, lyophilized powder, ≥550 units/mg protein (biuret)

Sigma-Aldrich

G8529

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides Type XXIII, ammonium sulfate suspension, 550-1,100 units/mg protein (biuret), ≥2.0 mg/mL Biuret

Sigma-Aldrich

G5760

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides lyophilized powder, >= 550 units/mg protein (biuret)

Sigma-Aldrich

G5885

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides

description

recombinant, expressed in E. coli, ammonium sulfate suspension, ≥550 units/mg protein (biuret)

description

recombinant, expressed in E. coli, lyophilized powder, ≥550 units/mg protein (biuret)

description

Type XXIII, ammonium sulfate suspension, 550-1,100 units/mg protein (biuret), ≥2.0 mg/mL Biuret

description

lyophilized powder, >= 550 units/mg protein (biuret)

specific activity

≥550 units/mg protein (biuret)

specific activity

≥550 units/mg protein (biuret)

specific activity

550-1,100 units/mg protein (biuret)

specific activity

>= 550 units/mg protein (biuret)

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

technique(s)

-

biological source

bacterial (Leuconostoc mesenteroides)

biological source

-

biological source

bacterial (Leuconostoc mesenteroides)

biological source

bacterial (Leuconostoc mesenteroides)

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

-

recombinant

-

form

ammonium sulfate suspension

form

lyophilized powder

form

ammonium sulfate suspension

form

lyophilized powder

mol wt

128 kDa

mol wt

-

mol wt

54 kDa

mol wt

128 kDa

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Storage Class

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    0000574102
    0000574099
    0000574104
    0000574103
    0000473185

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    Questions

    1. Hallo, wieviel beträgt das Volumen für 1 kU? Vielen Dank.

      1 answer

      1. The volume is lot specific and can be calculated using the data provided in the Certificate of Analysis.
        For example, lot 0000114274
        Units per mg Protein = 846
        Mg Protein per mL - 9
        Units per mL = 7614
        G8404-1000U = approx 132 ul

        Please see the link below to review this Certificate of Analysis:
        https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/G8404-10KU0000241288.pdf

        Helpful?

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