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G8404

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides

recombinant, expressed in E. coli, ammonium sulfate suspension, ≥550 units/mg protein (biuret)

Synonym(s):

Entner-Doudoroff enzyme, G6PD, G6PDH, NADP glucose 6-phosphate dehydrogenase, G-6-P-DH

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Size/SKUAvailabilityPrice
200 units

Available to ship TODAYfromMILWAUKEE

$76.00
1000 units

Available to ship TODAYfromMILWAUKEE

$167.00
2000 units

Available to ship TODAYfromMILWAUKEE

$271.00
10000 units

Available to ship TODAYfromMILWAUKEE

$920.00

About This Item

CAS Number:
9001-40-5
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
1.1.1.49 (BRENDA, IUBMB)
MDL number:
MFCD00081656
Specific activity:
≥550 units/mg protein (biuret)
Biological source:
bacterial (Leuconostoc mesenteroides)
Recombinant:
expressed in E. coli

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biological source

bacterial (Leuconostoc mesenteroides)

Quality Segment

300

recombinant

expressed in E. coli

form

ammonium sulfate suspension

specific activity

≥550 units/mg protein (biuret)

mol wt

128 kDa

storage condition

(Tightly closed)

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

UPI0021A4D550

foreign activity

NADH oxidase, NADPH oxidase, 6-phosphogluconic dehydrogenase, phosphoglucose isomerase, hexokinase ≤0.01%

storage temp.

2-8°C

General description

Glucose 6-phosphate dehydrogenase (G-6-P-DH) is a key regulatory enzyme in the first step of the pentose phosphate pathway. G-6-P-DH is a glycoprotein with a molecular mass of 128 kDa (gel filtration).G6PD is a dimer and consists of a single active site at each subunit.

Research area: Cell Signaling

Application

Glucose-6-phosphate dehydrogenase has been used:

  • to test ketose reductase activity in developing maize endosperm.
  • to determine the levels of mannose in coronary heart disease patient-derived serum
  • to study its activity on extracellular polymeric substance (EPS) extract to determine cell lysis through sonication
  • to determine the glucose uptake in cultured human muscle satellite cells

Biochem/physiol Actions

G6PD provides all cells with reducing power as nicotinamide adenine dinucleotide phosphate (NADPH) which enables the cells to balance oxidative stress. Mutations in the G6PD gene are associated with X-linked G6PD deficiency, a hereditary genetic defect such as neonatal jaundice and acute hemolytic anemia.
Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.

Physical form

Suspension in 3.2 M (NH4)2SO4 containing 50 mM Tris and 1 mM MgCl2, pH 7.5

Other Notes

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NAD at pH 7.8 at 30 °C.
The volume is lot specific and can be calculated using the data:
For example, lot 0000114274
Units per mg Protein = 846
Mg Protein per mL - 9
Units per mL = 7614
G8404-1000U = approx 132 ul

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This Item
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Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides recombinant, expressed in E. coli, ammonium sulfate suspension, ≥550 units/mg protein (biuret)

Sigma-Aldrich

G8404

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides lyophilized powder, >= 550 units/mg protein (biuret)

Sigma-Aldrich

G5885

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides

Glucose-6-phosphate Dehydrogenase, Leuconostoc mesenteroides Catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconate.

Sigma-Aldrich

346774

Glucose-6-phosphate Dehydrogenase, Leuconostoc mesenteroides

Glucose-6-phosphate Dehydrogenase from baker’s yeast (S. cerevisiae) Type IX, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian)

Sigma-Aldrich

G4134

Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae)

description

recombinant, expressed in E. coli, ammonium sulfate suspension, ≥550 units/mg protein (biuret)

description

lyophilized powder, >= 550 units/mg protein (biuret)

description

Catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconate.

description

Type IX, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian)

biological source

bacterial (Leuconostoc mesenteroides)

biological source

bacterial (Leuconostoc mesenteroides)

biological source

-

biological source

-

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

technique(s)

-

specific activity

≥550 units/mg protein (biuret)

specific activity

>= 550 units/mg protein (biuret)

specific activity

≥200 units/mg protein

specific activity

200-400 units/mg protein (modified Warburg-Christian)

form

ammonium sulfate suspension

form

lyophilized powder

form

lyophilized

form

lyophilized powder

recombinant

expressed in E. coli

recombinant

-

recombinant

-

recombinant

-

UniProt accession no.

UPI0021A4D550

UniProt accession no.

-

UniProt accession no.

-

UniProt accession no.

-

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

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Questions

  1. Hallo, wieviel beträgt das Volumen für 1 kU? Vielen Dank.

    1 answer

    1. The volume is lot specific and can be calculated using the data provided in the Certificate of Analysis.
      For example, lot 0000114274
      Units per mg Protein = 846
      Mg Protein per mL - 9
      Units per mL = 7614
      G8404-1000U = approx 132 ul

      Please see the link below to review this Certificate of Analysis:
      https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/G8404-10KU0000241288.pdf

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