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About This Item
description
Detection reagent 1: Colorless Detection reagent 2: Colorless Peroxidase labelled anti-mouse antibody: Colorless Peroxidase labelled anti-rabbit antibody: Colorless Blocking agent: White
feature
wetted part: no
packaging
pkg of 1 kit
manufacturer/tradename
Cytiva RPN2108
storage temp.
2-8°C
General description
ECL™ Western Blotting System uses horseradish peroxidase (HRP) conjugated anti-mouse and anti-rabbit antibodies for luminol-based detection of Western blots. Blots probed with mouse or rabbit primary antibodies are incubated with HRP-conjugated secondary antibodies. Addition of ECL™ Detection Reagents results in a chemiluminescent signal that can be captured on film or detected with a CCD camera.
Each system contains the following reagents, sufficient for detection of 1000 cm2 membrane:
Anti-mouse HRP conjugate, 100 μl
Anti-rabbit HRP conjugate, 100 μl
ECL™ Detection Reagents 1 and 2, 62.5 mL each
Blocking agent, 5 g.
Features and Benefits
- Detection of mouse and rabbit primary antibodies on western blots using HRP-conjugated secondary antibodies and ECL™ reagents.
- Optimized system provides rapid, sensitive results using simple protocols.
- Designed for use with nitrocellulose and PVDF membranes.
- High sensitivity: Up to 10 times more sensitive than colorimetric methods.
- Rapid: Results can be rapidly obtained on X-ray film, providing hard copy data and avoiding fading associated with colorimetric detection methods.
- Easy reprobing: Save time and materials by reprobing on membranes up to 10 times.
- Quantifiable: Linear response in the range 0.2 to 2 OD units using pre-flashed Hyperfilm ECL,
- Non-radioactive-no specialized facilities needed
- Proven performance-the most widely referenced chemiluminescent detection method with hundreds of publications worldwide
Preparation Note
Analysis Note
Legal Information
1 of 1
This Item | |||
|---|---|---|---|
| description Cytiva RPN2108 | description Cytiva RPN2106 | description Cytiva RPN2232 | description Cytiva RPN2236 |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. - | storage temp. - |
| packaging pkg of 1 kit | packaging pkg of 1 kit | packaging pkg of 1 kit | packaging pkg of 1 kit |
| description Detection reagent 1: Colorless Detection reagent 2: Colorless Peroxidase labelled anti-mouse antibody: Colorless Peroxidase labelled anti-rabbit antibody: Colorless Blocking agent: White | description - | description - | description - |
| feature wetted part: no | feature wetted part: no | feature - | feature - |
| manufacturer/tradename Cytiva RPN2108 | manufacturer/tradename Cytiva RPN2106 | manufacturer/tradename Cytiva RPN2232 | manufacturer/tradename Cytiva RPN2236 |
Signal Word
Warning
Hazard Codes
Precautionary Statements
Storage Class
12 - Non Combustible Liquids
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