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H1506

3α-Hydroxysteroid Dehydrogenase from Pseudomonas testosteroni

≥15 units/mg protein

Synonym(s):

3α-Hydroxysteroid:NAD(P)+ oxidoreductase

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50 units
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$716.00

About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-839-5
MDL number:
EC Number:
Specific activity:
≥15 units/mg protein
Biological source:
bacterial (Pseudomonas testosteroni)

$716.00


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biological source

bacterial (Pseudomonas testosteroni)

Quality Segment

form

lyophilized solid

specific activity

≥15 units/mg protein

purified by

chromatography

composition

Protein, ≥25%

foreign activity

β-hydroxysteroid dehydrogenase ≤0.5%, alcohol dehydrogenase ≤0.05%

storage temp.

−20°C

Application

3α-Hydroxysteroid Dehydrogenase from Pseudomonas testosterone has been used:
  • in the preparation of composite electrode platform to for the determination of androsterone
  • in potassium phosphate buffer for the bioluminescent assay of bile acids in serum
  • for total bile salts assay using an enzymatic method

Biochem/physiol Actions

3α-hydroxysteroid dehydrogenase (3α-HSD) may help to modulate the occupancy of the androgen receptor (AR) in androgen target tissues. It may also act in hindering androgen action.

Physical form

Lyophilized powder containing potassium phosphate buffer salt, EDTA, and DL-dithiothreitol

Other Notes

One unit will oxidize 1.0 μmole of androsterone per min at pH 8.9 at 25 °C in the presence of β-NAD+.

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This Item
L3888G3512F8649
description

≥15 units/mg protein

description

lyophilized powder, 150-500 units/mg protein

description

lyophilized powder, ≥50 units/mg protein

description

lyophilized powder, 5.0-15.0 units/mg protein

specific activity

≥15 units/mg protein

specific activity

150-500 units/mg protein

specific activity

≥50 units/mg protein

specific activity

5.0-15.0 units/mg protein

biological source

bacterial (Pseudomonas testosteroni)

biological source

bacterial (Lactobacillus leichmannii)

biological source

bacterial (Cellulomonas spp.)

biological source

fungus (Candida boidinii)

form

lyophilized solid

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

foreign activity

alcohol dehydrogenase ≤0.05%, β-hydroxysteroid dehydrogenase ≤0.5%

foreign activity

Malic dehydrogenase <0.5% of base activity

foreign activity

-

foreign activity

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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