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Monoclonal Anti-Heat Shock Protein 70 antibody produced in mouse

Name: Monoclonal Anti-Heat Shock Protein 70 antibody produced in mouse
Brand: SIGMA
Price: 469.2 USD
Availability: InStock

clone BRM-22, ascites fluid

Synonym(s):

HSP70 Antibody - Monoclonal Anti-Heat Shock Protein 70 antibody produced in mouse, Hsp70 Antibody

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Size/SKU	Availability	Price
100 μL	Available to ship TODAYfromMILWAUKEE	$552.00 $469.20
0.2 mL	Available to ship TODAYfromMILWAUKEE	$727.00 $617.95

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00164609

Conjugate:

unconjugated

Clone:

BRM-22, monoclonal

Application:

ARR, ELISA (i), EM, IHC (f), WB

Citations:

104

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

BRM-22, monoclonal

mol wt

antigen 70 kDa

species reactivity

bovine, chicken, guinea pig, plant, Drosophila, rat, human, nematode, rabbit, hamster

technique(s)

electron microscopy: suitable, immunohistochemistry (frozen sections): suitable, indirect ELISA: suitable, microarray: suitable, western blot: 1:5,000 using bovine brain extract

isotype

IgG1

UniProt accession no.

P0DMV8

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... HSPA1A(3303), HSPA1B(3304)
rat ... Hspa1a(24472), Hspa1b(294254)

Description

General description

A variety of environmental disruptions, such as a sudden increase in temperature, induce cells to rapidly synthesize a group of polypeptides known as heat shock (stress) proteins. Eukaryotic cells contain a multigene family that encodes several closely related 70 kD stress proteins (the HSP70 family) that differ in their intracellular location and regulation.These include four proteins: the constitutive (or cognate) HSP73, the stress-inducible HSP72 and the glucose regulated proteins grp78 (or BiP) and grp75.

Monoclonal Anti-Heat Shock Protein 70 (mouse IgG1 isotype) is derived from the BRM-22 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified bovine brain HSP70. Heat shock protein family A (Hsp70) member 1A (HSPA1A), is encoded by the gene mapped to human chromosome 6p21.33. It is widely expressed in all tissues. The encoded protein is characterized with an N-terminal ATPase domain of 45 kDa and a C-terminal substrate binding domain of 25 kDa.

Immunogen

HSP70 isolated from bovine brain

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)
Western Blotting (4 papers)

Monoclonal Anti-Heat Shock Protein 70 antibody produced in mouse has been used in:

enzyme-linked immunosorbent assay (ELISA)
immunoblot
dot blot
immunocytochemistry

The antibody titer of at least 1:5,000 was determined by immunoblotting using bovine brain extract.

Biochem/physiol Actions

By immunoblotting, the antibody localizes both the constitutive (HSP73) and inducible (HSP72) forms of HSP70. The antibody recognizes HSP70 from brain and other tissue. Immunofluorescent staining demonstrates a rapid and reversible accumulation of the HSP70 protein within the nucleus of heat-shocked (42 °C, 1 hour) human fibroblasts.

Members of the HSP70 family play a major role in the folding, unfolding and translocation of polypeptides as well as in the assembly and disassembly of oligomeric protein complexes. In addition, several possible roles have been attributed to the HSP70 family of proteins, in the immune response. It has been shown that alcoholic liver disease is associated with intracytoplasmic accumulation of HSP70. HSP72 was found to increase dramatically in the brains of Alzheimer′s disease patient, and was localized exclusively in neuritic plaques and neurofibrillary tangles. HSP70 concentrates in nuclei during heat shock and returns to the cytoplasm when the shock is removed.

Other Notes

This product can be found as purified product that was produced using cell culture hybridoma product.
SAB4200714 Anti-Heat Shock Protein 70 (HSP70) antibody, Mouse monoclonal
clone BRM-22, purified from hybridoma cell culture

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	SAB1403949	SAB3701436	SAB5700631
Sigma-Aldrich H5147 Monoclonal Anti-Heat Shock Protein 70 antibody produced in mouse	Sigma-Aldrich SAB1403949 Monoclonal Anti-HSPA1B antibody produced in mouse	Sigma-Aldrich SAB3701436 Monoclonal Anti-Hsc70 (Hsp73) antibody produced in mouse	Sigma-Aldrich SAB5700631 Anti-HSP70 antibody produced in rabbit
description clone BRM-22, ascites fluid	description clone 2D11, purified immunoglobulin, buffered aqueous solution	description clone N27F3-4, purified immunoglobulin	description -
clone BRM-22, monoclonal	clone 2D11, monoclonal	clone N27F3-4, monoclonal	clone polyclonal
biological source mouse	biological source mouse	biological source mouse	biological source rabbit
antibody form ascites fluid	antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form affinity isolated antibody
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
species reactivity bovine, chicken, guinea pig, plant, Drosophila, rat, human, nematode, rabbit, hamster	species reactivity rat, human, mouse	species reactivity canine, bovine, salmon, beluga, rat, Caenorhabditis elegans, human, carp, Drosophila, mouse, chicken	species reactivity mouse, human, rat
Gene Information human ... HSPA1A(3303), HSPA1B(3304) rat ... Hspa1a(24472), Hspa1b(294254)	Gene Information human ... HSPA1B(3304)	Gene Information human ... HSPA1A(3303)	Gene Information human ... HSPA1A(3303)

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c