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Anti-MAP2 Antibody

Name: Anti-MAP2 Antibody
Brand: SIGMA
Price: 192 USD
Availability: InStock

mouse monoclonal, AP-20

Synonym(s):

Anti-MAP2 (2a+2b) antibody, Mouse monoclonal, Anti-Microtubule Associated Protein 2

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Size/SKU	Availability	Price
25 μL	Available to ship TODAYfromMILWAUKEE	$192.00
100 μL	Available to ship TODAYfromMILWAUKEE	$764.00
200 μL	Available to ship TODAYfromMILWAUKEE	$933.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00164684

Conjugate:

unconjugated

Clone:

AP-20, monoclonal

Application:

ARR, ICC, WB

Citations:

$192.00

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Properties

Product Name

Anti-MAP2 (2a+2b) antibody, Mouse monoclonal, ~2 mg/mL, clone AP-20, purified from hybridoma cell culture

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

AP-20, monoclonal

form

buffered aqueous solution

mol wt

apparent mol wt 280 kDa

species reactivity

Xenopus, mouse, quail, human, bovine, rat, aquatic salamander

packaging

antibody small pack of 25 μL

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable, microarray: suitable, western blot: 1-3 μg/mL using rat brain preparation or rat cerebral cortex extract

isotype

IgG1

UniProt accession no.

P11137

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MAP2(4133)
mouse ... Mtap2(17756)
rat ... Map2(25595)

Description

General description

MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). In the newborn rat brain, MAP2b and MAP2c are present, while MAP2a is absent. Between postnatal days 10 and 20 MAP2a appears, at levels equal to that of MAP2b. At the same time, the level of MAP2c drops by 10-fold. This change happens during the period when dendrite growth is completed and when neurons have reached their mature morphology. MAP2 is degraded by a Cathepsin D like protease in the brain of aged rats. There is some indication that MAP2 is expressed at higher levels in some types of neurons than in others.

Monoclonal Anti-MAP2 (2a + 2b) (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with bovine microtubule associated protein 2 (MAP2).

Monoclonal Anti-MAP2 specificity was established by an immunoblot. The antibody localizes the high molecular weight forms of MAP2, namely MAP2a and MAP2b, but shows no reactivity with MAP2c. No crossreactivity is observed with MAP1, MAP5, tubulin, or tau (t).

Immunogen

bovine MAP2

Application

Monoclonal Anti-MAP2 (2a+2b) antibody produced in mouse has been used in immunocytochemistry.

Mouse monoclonal clone AP-20 anti-MAP2 (2a+2b) antibody is used to tag MAP2 for detection and quantitation by Western blotting and immunohistochemical (IHC) techniques. It is used as a probe to determine the roles of MAP2a and MAP2b in cell signaling especially in neural tissues.

Biochem/physiol Actions

Microtubule associated protein 2 (MAP2) is known to promote microtubule assembly and to form side arms on microtubules. It also interacts with neurofilaments, actin and other elements of the cytoskeleton. MAP2 is degraded by a cathepsin D like protease in the brain of aged rats. There is some indication that MAP2 is expressed at higher levels in some types of neurons than in others. MAP2 is known to promote microtubule assembly and to form side-arms on microtubules. It also interacts with neurofilaments, actin and other elements of the cytoskeleton.

Monoclonal Anti-MAP2 antibody reacts with human, bovine, rat, mouse, Xenopus, salamander, and quail tissue or cells by immunocytochemical techniques utilizing either a fluorescent or peroxidase label, or by immunohistochemistry.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	M9942	MAB378	AMAB91375
Sigma-Aldrich M2320 Anti-MAP2 (2a+2b) antibody, Mouse monoclonal	Sigma-Aldrich M9942 Monoclonal Anti-MAP2 antibody produced in mouse	Sigma-Aldrich MAB378 Anti-MAP2A Antibody, AP20	Sigma-Aldrich AMAB91375 Monoclonal Anti-MAP2 antibody produced in mouse
description ~2 mg/mL, clone AP-20, purified from hybridoma cell culture	description clone HM-2, purified from hybridoma cell culture	description ascites fluid, clone AP20, Chemicon^®	description Prestige Antibodies^® Powered by Atlas Antibodies, clone CL5420, purified immunoglobulin, buffered aqueous glycerol solution
antibody form purified from hybridoma cell culture	antibody form purified immunoglobulin	antibody form ascites fluid	antibody form purified immunoglobulin
clone AP-20, monoclonal	clone HM-2, monoclonal	clone AP20, monoclonal	clone CL5420, monoclonal
Gene Information human ... MAP2(4133) mouse ... Mtap2(17756) rat ... Map2(25595)	Gene Information human ... MAP2(4133) mouse ... Mtap2(17756) rat ... Map2(25595)	Gene Information human ... MAP2(4133)	Gene Information human ... MAP2(4133)
biological source mouse	biological source mouse	biological source mouse	biological source mouse
species reactivity Xenopus, mouse, quail, human, bovine, rat, aquatic salamander	species reactivity rat, chicken, human, mouse, bovine, quail	species reactivity quail, bovine, human, mouse, amphibian, Xenopus, rat, avian	species reactivity human
conjugate unconjugated	conjugate unconjugated	conjugate -	conjugate unconjugated

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Storage Class

10 - Combustible liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)