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Anti-Neurocan antibody, Mouse monoclonal

Name: Anti-Neurocan antibody, Mouse monoclonal
Brand: SIGMA
Price: 639 USD
Availability: InStock

clone 650.24, purified from hybridoma cell culture

Synonym(s):

Anti-CSPG3, Anti-Chondroitin Sulfate Proteogycan 3

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Size/SKU	Availability	Price
200 μL	Check Cart for Availability	$639.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD02263808

Conjugate:

unconjugated

Clone:

650.24, monoclonal

Application:

ELISA (i), ICC, IHC (f), WB

Citations:

$639.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

650.24, monoclonal

form

buffered aqueous solution

mol wt

antigen 260 kDa (additional band at 160 kDa)

species reactivity

rat

packaging

antibody small pack of 25 μL

concentration

~1 mg/mL

technique(s)

immunocytochemistry: suitable, immunohistochemistry (frozen sections): suitable, indirect ELISA: suitable, western blot: 0.1-0.2 μg/mL using neonatal rat brain treated with chondroitinase.

isotype

IgG1

UniProt accession no.

P55067

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

rat ... Ncan(58982)

Description

General description

Monoclonal Anti-Neurocan (mouse IgG1 isotype) is derived from the hybridoma 650.24 produced by the fusion of mouse myeloma cells (P3X cells) and splenocytes from BALB/c mice immunized with embryonic rat brain proteoglycans. Neurocan is a chondroitin sulfate proteoglycan (known also as CSPG3). It accounts for 20−30% of chondroitin sulfate proteoglycans in early postnatal rat brain. The mouse neurocan cDNA encodes for a 1,257-amino acid protein with a 22-amino acid signal peptide followed by an immunoglobulin-like domain and repeating motifs characteristic of the hyaluronic acid-binding region of aggregating proteoglycans. Neurocan is found in large amounts in glial scar, a primarily astrocytic structure formed after an injury in the central nervous system (CNS).

Neurocan is a chondroitin sulfate proteoglycan (knownalso as CSPG3) thought to be involved in the modulation of cell adhesion and migration. It accounts for 20-30% of chondroitin sulfate proteoglycans in early postnatal rat brain.

Neurocan is a proteoglycan which is expressed in the nervous tissue.

Immunogen

embryonic rat brain proteoglycans.

Application

Anti-Neurocan antibody, Mouse monoclonal has been used in:

western blotting
immunofluorescence
enzyme-linked immunosorbent assay (ELISA)
immunocytochemistry
immunohistochemistry

Biochem/physiol Actions

Neurocan can inhibit the growth of axons. In presence of central nervous system injuries, it also takes part in tissue repair and neural network reconstruction.

Neurocan participates in the modulation of cell adhesion and migration. It exerts a negative effect on growing cerebellar axons.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	I0282	SAB5200871	SAB5200880
Sigma-Aldrich N0913 Anti-Neurocan antibody, Mouse monoclonal	Sigma-Aldrich I0282 Anti-α-Internexin antibody, Mouse monoclonal	Sigma-Aldrich SAB5200871 Monoclonal Anti-Brevican - Atto 390 antibody produced in mouse	Sigma-Aldrich SAB5200880 Monoclonal Anti-Brevican - Apc antibody produced in mouse
description clone 650.24, purified from hybridoma cell culture	description clone 2E3, purified from hybridoma cell culture	description clone S294A-6, purified immunoglobulin	description clone S294A-6, purified immunoglobulin
Gene Information rat ... Ncan(58982)	Gene Information human ... INA(9118) rat ... Ina(24503)	Gene Information rat ... Bcan(25393)	Gene Information rat ... Bcan(25393)
conjugate unconjugated	conjugate unconjugated	conjugate Atto 390 conjugate	conjugate Allophycocyanin conjugate
species reactivity rat	species reactivity feline, rat, bovine, human, pig	species reactivity mouse, rat	species reactivity rat, mouse
clone 650.24, monoclonal	clone 2E3, monoclonal	clone S294A-6, monoclonal	clone S294A-6, monoclonal
antibody form purified from hybridoma cell culture	antibody form purified from hybridoma cell culture	antibody form purified immunoglobulin	antibody form purified immunoglobulin
biological source mouse	biological source mouse	biological source mouse	biological source mouse

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Storage Class

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)