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Anti-β-O-Linked N-Acetylglucosamine antibody, Mouse monoclonal

Name: Anti-β-O-Linked N-Acetylglucosamine antibody, Mouse monoclonal
Brand: SIGMA
Price: 926 USD
Availability: InStock

clone CTD110.6, purified from hybridoma cell culture

Synonym(s):

Anti-O-GLcNAc, Anti-OGT

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Size/SKU	Availability	Price
200 μL	Check Cart for Availability	$926.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD07370422

Conjugate:

unconjugated

Clone:

CTD110.6, monoclonal

Application:

ELISA (i), ICC, IHC, IP, WB

Citations:

$926.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

CTD110.6, monoclonal

form

buffered aqueous solution

species reactivity

wide range

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot: 0.2-0.4 μg/mL using total cell extract of HeLa cells

isotype

IgM

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... OGT(8473)

Description

General description

Monoclonal Anti-β-O-Linked N-Acetylglucosamine (mouse IgM isotype) is derived from the hybridoma CTD110.6 produced by the fusion of mouse myeloma cells (P3X63-Ag8.653 cells) and cells from BALB/c mice immunized with a synthetic peptide YSPTS(O-GlcNAc)PSK conjugated to KLH. This antibody recognizes O-GlcNAc in β-O-glycosidic linkage to both serine and threonine and does not cross react with α-linked Ser/Thr-O-GlcNAc, α-linked Ser-O-linked N-acetylgalactosamine or N-linked oligosaccharides on ovalbumin and immunoglobulin G. O-linked N-acetylglucosamine (GlcNAc) transferase (OGT) has 13.5 tetratricopeptide-repeats (TPR) and a globular catalytic domain. The gene encoding this protein is localized on human chromosome Xq13.1.

Immunogen

synthetic peptide YSPTS(O-GlcNAc)PSK.

Application

Monoclonal Anti-β-O-Linked N-Acetylglucosamine antibody produced in mouse has been used in Western Blotting.

Biochem/physiol Actions

O-linked N-acetylglucosamine (GlcNAc) transferase (OGT) has a role in the addition of O-linked N-acetylglucosamine (O-GlcNAc) to serine/threonine residues in the protein of interest. It is crucial for O-GlcNAcylation of RNA polymerase II. Absence of OGT has been shown to reduce transcription and pol II promoter occupancy. The gene is upregulated in breast cancer tissues, colon cancer, lung cancer and laryngeal cancer. This protein might be associated with tumorigenesis and metastasis.

The antibody recognizes O-GlcNAc in β-O-glycosidic linkage to both serine and threonine and does not cross reacts with α-linked Ser/Thr-O-GlcNAc, α-linked Ser-O-linked N-acetylgalactosamine or N-linked oligosaccharides on ovalbumin and immunoglobulin G.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	MABS157	MABS1254	SAB5202310
Sigma-Aldrich O7764 Anti-β-O-Linked N-Acetylglucosamine antibody, Mouse monoclonal	Sigma-Aldrich MABS157 Anti-O-Linked N-Acetylglucosamine Antibody, clone RL2	Sigma-Aldrich MABS1254 Anti-O-GlcNAc Antibody, clone CTD110.6	Sigma-Aldrich SAB5202310 Monoclonal Anti-O-GlcNAc antibody produced in mouse
description clone CTD110.6, purified from hybridoma cell culture	description clone RL2, from mouse	description clone CTD110.6, from mouse	description clone 9H6
species reactivity wide range	species reactivity -	species reactivity -	species reactivity human
clone CTD110.6, monoclonal	clone RL2, monoclonal	clone CTD110.6, monoclonal	clone 9H6, monoclonal
biological source mouse	biological source mouse	biological source mouse	biological source mouse
Gene Information human ... OGT(8473)	Gene Information human ... OGT(8473)	Gene Information human ... OGT(8473)	Gene Information human ... OGT(8473)
antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified immunoglobulin
conjugate unconjugated	conjugate -	conjugate -	conjugate unconjugated

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Storage Class

10 - Combustible liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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