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Anti-Prion Protein antibody, Mouse monoclonal

Name: Anti-Prion Protein antibody, Mouse monoclonal
Brand: SIGMA
Price: 672 USD
Availability: InStock

clone 8H4, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-Prion Protein antibody produced in mouse, Anti-AA960666, Anti-CD230, Anti-PRIP, Anti-PrP, Anti-PrP<C>, Anti-PrPSc, Anti-Prn-i, Anti-RP23-401J24.1, Anti-Sinc

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Size/SKU	Availability	Price
200 μL	Available to ship TODAYfromMILWAUKEE	$672.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD01865558

Conjugate:

unconjugated

Clone:

8H4, monoclonal

Application:

ELISA (i), EM, FACS, ICC, IHC, IP, WB

Citations:

$672.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

8H4, monoclonal

form

buffered aqueous solution

mol wt

antigen 25-35 kDa

species reactivity

bovine, mouse, monkey, human, sheep, rat

packaging

antibody small pack of 25 μL

concentration

~1.5 mg/mL

technique(s)

electron microscopy: suitable, flow cytometry: suitable, immunohistochemistry: suitable, indirect ELISA: suitable, western blot: 2-4 μg/mL using mouse brain extract, immunocytochemistry: suitable, immunoprecipitation (IP): suitable

isotype

IgG2b

UniProt accession no.

P04156

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

bovine ... PRNP(281427)
human ... PRNP(5621)
mouse ... Prnp(19122)
rat ... Prnp(24686)
sheep ... PRNP(493887)

Description

General description

Anti-Prion Protein antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the hybridoma 8H4 produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from Prnp-/- mice immunized with recombinant murine PrPc. Prion protein (PrP) is mapped to human chromosome 20p13. PrP is constitutively expressed in brain and other tissues of healthy humans and animals and is present in high levels at the synapse.

Prion is a cell surface glycoprotein present in two isoform- PrPc (a cellular isoform) and PrPsc (a disease associated isoform). Monoclonal anti-prion protein antibody is useful for the treatment of prion disease, by inhibiting the abnormal isoform PrPsc . This antibody can also be used for passive immunization of animals to protect them from prion infection. Anti prion protein antibody may be used to inhibit abnormal prion protein accumulation in cultured scrapie-infected neuroblastoma cells. The antibody reacts specifically with amino acids 145-180 of human prion. It also has specificity for monkey, cow, sheep, deer, squirrel, hamster, mouse and rat.

Immunogen

Recombinant MoPrp residues 121-231

recombinant mouse PrP^C.

Application

Anti-Prion Protein antibody, mouse monoclonal may be used in:

immunoblotting
flow cytometry
immunocytochemistry
immunoprecipitation
immunoelectron microscopy
immunohistochemistry
enzyme linked immunosorbent assay

Biochem/physiol Actions

Prion-related diseases are fatal neurodegenerative disorders also known as transmissible spongiform encephalopathies (TSEs). Prion plaques are of three types: unicentric (single, compact core), multicentric (two or more cores and definite border), and diffuse plaques without a definite central core. Disease-associated prion protein specifically inhibits the proteolytic β-subunits of the 26S proteasome. This may clarify the mechanism of cell death by the prion protein. Prion protein may be involved in copper utilization, serving to buffer copper at the synaptic cleft or to mediate re-uptake of copper into the presynapse. Alternatively, bound copper may influence PrP binding characteristics. The PrP-copper complex may be crucial for synaptic homeostasis as a result of its antioxidant activity.

The antibody epitope resides within amino acids 145-180 of human prion.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	MAB5424	MABN2298M	MAB1562
Sigma-Aldrich P0110 Anti-Prion Protein antibody, Mouse monoclonal	Sigma-Aldrich MAB5424 Anti-Prion Protein Antibody	Sigma-Aldrich MABN2298M Anti-Prion Protein Antibody, clone POM2	Sigma-Aldrich MAB1562 Anti-Prion Protein Antibody, a.a. 109-112, clone 3F4
description clone 8H4, purified from hybridoma cell culture	description Chemicon^®, from mouse	description clone POM2, from mouse	description clone 3F4, Chemicon^®, from mouse
Gene Information bovine ... PRNP(281427) human ... PRNP(5621) mouse ... Prnp(19122) rat ... Prnp(24686) sheep ... PRNP(493887)	Gene Information human ... PRNP(5621)	Gene Information mouse ... Prnp(19122)	Gene Information human ... PRNP(5621)
biological source mouse	biological source mouse	biological source mouse	biological source mouse
clone 8H4, monoclonal	clone monoclonal	clone POM2, monoclonal	clone 3F4, monoclonal
species reactivity bovine, mouse, monkey, human, sheep, rat	species reactivity sheep, mouse, mule deer, bovine, elk	species reactivity mouse, human	species reactivity hamster, human
antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified immunoglobulin
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c