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Monoclonal Anti-Splicing Factor SC-35 antibody produced in mouse

Name: Monoclonal Anti-Splicing Factor SC-35 antibody produced in mouse
Brand: SIGMA
Price: 552 USD
Availability: InStock

clone SC-35, ascites fluid

Synonym(s):

Anti-SC-35, Anti-SC35

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Size/SKU	Availability	Price
100 μL	Available to ship TODAYfromMILWAUKEE	$552.00
0.2 mL	Available to ship TODAYfromMILWAUKEE	$763.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00282472

Conjugate:

unconjugated

Clone:

SC-35, monoclonal

Application:

ELISA (i), EM, ICC, IF, IP

Citations:

107

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

SC-35, monoclonal

mol wt

antigen 35 kDa (a doublet)

contains

15 mM sodium azide

species reactivity

frog, Drosophila, newt, rat, human

technique(s)

electron microscopy: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, indirect immunofluorescence: 1:2,000 using cultured human fibroblasts

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SFRS2(6427)
rat ... Sfrs2(494445)

Description

General description

Monoclonal Anti-Splicing Factor SC-35 (mouse IgG1 isotype) is derived from the SC-35 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized RBF-DNJ mouse. Splicing component of 35 kDa (SC-35) is also termed as PR264. SC-35 displays a speckled distribution in the nucleus that co-localizes with small nuclear ribonucleoproteins (snRNPs), but unlike snRNPs, SC-35 does not give diffuse nuclear labeling.

Nuclear pre-mRNA splicing takes place in a multi-component structure termed a spliceosome. Major subunits of spliceosomes are U1, U2, U4/U6 and U5 small nuclear ribonucleoproteins (snRNP′s). In addition to the snRNP′s, a number of protein factors have been identified which are required for spliceosome assembly and splicing. For example, the protein factors U2AF, SF2 and SF3 are required for the binding of the U2 snRNP to the intron branch-point and for assembly of the pre-splicing complex. Two other non-snRNP splicing factors, SF2/ASF and SC-35 (splicing component of 35 kD, also termed PR264), are both required for the first step of splicing and spliceosome assembly. SF2/ASF and SC-35 are also involved in 5N splice site selection of alternatively spliced pre-mRNA′s.
The essential non-snRNP splicing factor SC-35 displays a speckled distribution in the nucleus that co-localizes with snRNPs, but unlike snRNPs, SC-35 does not give diffuse nuclear labelling. In the nucleus, snRNPs are concentrated in coiled bodies and in the speckled regions, whereas SC-35 is found in speckles but not in coiled bodies.

Immunogen

partially purified mammalian splicesome.

Application

Monoclonal Anti-Splicing Factor SC-35 antibody produced in mouse has been used in:

fluorescence image analysis
immunohistochemistry
two or three color immunofluorescence staining
enzyme-linked immunosorbent assay (ELISA)
immunohistology
immunoelectronmicroscopy
immunoaffinity purification
immunoprecipitation

Monoclonal Anti-Splicing Factor SC-35 may be used for the localization of SC-35 using ELISA, immunohistology and immunoelectronmicroscopy, for inhibition and depletion of splicing activity in nuclear extracts, and for immunoaffinity purification and immunoprecipitation.
Indirect immunofluorescence: a dilution of at least 1:2,000 was determined by staining cultured human fibroblasts.

Biochem/physiol Actions

Recognizes a phospho-epitope on the non-snRNP (small nuclear ribonucleoprotein particles) factor SC-35. The antibody reacts with the splicing factor SC-35 and with the SC-35-related non-snRNP factor SF2/ASF. The antibody labels SC-35 as a speckled pattern in the nucleoplasm, excluding the nucleoli. Other uses include inhibition and depletion of splicing activity in nuclear extracts and immunoaffinity purification.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Show Differences

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This Item	SAB4200725	SAB2109143	HPA049905
Sigma-Aldrich S4045 Monoclonal Anti-Splicing Factor SC-35 antibody produced in mouse	Sigma-Aldrich SAB4200725 Anti-Splicing Factor SC-35 antibody, Mouse monoclonal	Sigma-Aldrich SAB2109143 Anti-SFRS2 (C-terminal) antibody produced in rabbit	Sigma-Aldrich HPA049905 Anti-SRSF2 antibody produced in rabbit
description clone SC-35, ascites fluid	description clone SC-35, purified from hybridoma cell culture	description affinity isolated antibody	description Prestige Antibodies^® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
species reactivity frog, Drosophila, newt, rat, human	species reactivity human, mouse, quail, hamster, frog, porcine, rat	species reactivity -	species reactivity human
Gene Information human ... SFRS2(6427) rat ... Sfrs2(494445)	Gene Information human ... SRSF2(6427)	Gene Information human ... SRSF2(6427)	Gene Information human ... SRSF2(6427)
biological source mouse	biological source mouse	biological source rabbit	biological source rabbit
antibody form ascites fluid	antibody form purified from hybridoma cell culture	antibody form affinity isolated antibody	antibody form affinity isolated antibody
clone SC-35, monoclonal	clone SC-35, monoclonal	clone polyclonal	clone polyclonal

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Storage Class

10 - Combustible liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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