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| Size/SKU | Availability | Price |
|---|---|---|
50 μg | Check Cart for Availability | $226.00 |
About This Item
NACRES:
NA.25
UNSPSC Code:
41105335
$226.00
Check Cart for Availability
Quality Segment
form
liquid
usage
100 uses
suitability
suitable for electrophoresis (DNA)
storage temp.
−20°C
General description
Sigma′s 50 bp DNA Step Ladder contains 17 blunt-ended fragments consisting of 50 bp repeats from 50 to 500 bp, 100 bp repeats from 600 to 900 bp, and 1 kb repeats from 1 to 3 kb. For NA electrophoresis, 10 μl of the marker should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel. Suitable for Northern and Southern blotting.
Application
Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels.
Other Notes
Contains blunt-ended fragments of DNA, 50-500 bp by 50, 600-900 bp by 100, and 1000-3000 bp by 1000 (17 bands).
For optimal resolution, the recommended agarose gel concentration is 2.0%.
Sigma′s 50 bp DNA Step Ladder is provided in a solution of 10 mM Tris-HCl (pH 7.5), 10 mM NaCl, and 1 mM EDTA.
1 of 1
This Item | |||
|---|---|---|---|
| description for DNA electrophoresis | description ready-to-use marker for DNA electrophoresis | description for DNA electrophoresis | description for electrophoresis of PCR fragments |
| form liquid | form (liquid) | form liquid | form liquid |
| Quality Level 100 | Quality Level 200 | Quality Level 200, 100 | Quality Level 100 |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| usage 100 uses | usage sufficient for 100 loads | usage 100 uses (0.1 mg size), 250 uses (250 μg size) | usage 80 uses |
| suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) |
ladder | ladder, marker, NA electrophoresis | ladder, NA electrophoresis, marker | - |
Storage Class
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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