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S7025

Sigma-Aldrich

50 bp DNA Step Ladder

for DNA electrophoresis

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Synonym(s):
DNA Ladder, Step Ladder, 50 bp, 17 Fragments, 50-3000 bp
NACRES:
NA.25

Quality Level

form

liquid

usage

100 uses

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

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This Item
D3812D0428P9577
50 bp DNA Step Ladder for DNA electrophoresis

S7025

50 bp DNA Step Ladder

DirectLoad™ 50 bp DNA Step Ladder ready-to-use marker for DNA electrophoresis

D3812

DirectLoad 50 bp DNA Step Ladder

1 kb DNA Ladder for DNA electrophoresis

D0428

1 kb DNA Ladder

PCR 50 - 2,000 bp Marker for electrophoresis of PCR fragments

P9577

PCR 50 - 2,000 bp Marker

Quality Level

100

Quality Level

200

Quality Level

100, 200

Quality Level

200

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

usage

100 uses

usage

sufficient for 100 loads

usage

-

usage

sufficient for 50 uses

suitability

suitable for electrophoresis (DNA)

suitability

suitable for electrophoresis (DNA)

suitability

suitable for electrophoresis (DNA)

suitability

suitable for electrophoresis (DNA)

NA electrophoresis

NA electrophoresis, ladder, marker

Ladder, Marker, NA electrophoresis

-

General description

Sigma′s 50 bp DNA Step Ladder contains 17 blunt-ended fragments consisting of 50 bp repeats from 50 to 500 bp, 100 bp repeats from 600 to 900 bp, and 1 kb repeats from 1 to 3 kb. For NA electrophoresis, 10 μl of the marker should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel. Suitable for Northern and Southern blotting.

Application

Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels.

Components

Sigma′s 50 bp DNA Step Ladder is provided in a solution of 10 mM Tris-HCl (pH 7.5), 10 mM NaCl, and 1 mM EDTA.

Other Notes

Contains blunt-ended fragments of DNA, 50-500 bp by 50, 600-900 bp by 100, and 1000-3000 bp by 1000 (17 bands).
For optimal resolution, the recommended agarose gel concentration is 2.0%.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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T1503
Product Number
-
25G
Pack Size/Quantity

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705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

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Articles

We offers a variety of markers that aid size determination of samples separated by agarose and/or polyacrylamide gel electrophoresis. These products include markers for DNA, PCR fragments and RNA and can be concurrently run with the samples. All the markers stain well with common nucleic acid stains.

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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