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Anti-HRD1/SYVN1 antibody, Mouse monoclonal

Name: Anti-HRD1/SYVN1 antibody, Mouse monoclonal
Brand: SIGMA
Price: 564 USD
Availability: InStock

clone HRD1-5, purified from hybridoma cell culture

Synonym(s):

Anti-E3 ubiquitin-protein ligase synoviolin, Anti-Synovial apoptosis inhibitor 1, Anti-Synoviolin

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Size/SKU	Availability	Price
200 μL	Check Cart for Availability	$564.00

About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

HRD1-5, monoclonal

Application:

IP, WB

Citations:

$564.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

HRD1-5, monoclonal

form

buffered aqueous solution

mol wt

antigen ~70 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 5-10 μg using lysates of human HEK-293T cells, western blot: 2-4 μg/mL using whole extracts of HEK-293T cells over-expressing human HRD1/SYVN1

isotype

IgG1

UniProt accession no.

Q86TM6

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SYVN1(84447)

Description

General description

Monoclonal Anti-HRD1/SYVN1 (mouse IgG1 isotype) is derived from the hybridoma HRD1-5 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the C-terminal region of human HRD1/SYVN1 conjugated to KLH. β-Hydroxy β-methylglutaryl-CoA (HMG-CoA) reductase degradation protein (HRD1)/synoviolin (SYVN1) is an endoplasmic reticulum (ER)-membrane resident E3 ubiquitin ligase. It has a five-transmembrane domain, a really interesting new gene (RING)-finger domain and a proline-rich domain.

Synoviolin 1 (SYVN1), also referred as 3-hydroxy-3-methylglutaryl-CoA reductase degradation protein 1 (HRD1), is encoded by the gene mapped to human chromosome 11q13.1. It is mainly expressed in liver, spleen, prostate and pancreas.

Immunogen

a synthetic peptide corresponding to the C-terminal region of human HRD1/SYVN1, conjugated to KLH. The corresponding sequence differs by two amino-acids in rat and mouse.

Application

Anti-HRD1/SYVN1 antibody, Mouse monoclonal has been used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

β-Hydroxy β-methylglutaryl-CoA (HMG-CoA) reductase degradation protein (HRD1)/synoviolin (SYVN1) protects against ER stress-induced apoptosis through ER stress-associated protein degradation (ERAD). The (RING)-finger domain mediates the transfer of ubiquitin from E2 to substrates. HRD1 expression is strongly induced by ER stress. It mediates proteasomal degradation via p53 tumor suppressor gene. Overexpression of HRD1 is implicated in the pathogenesis of rheumatoid arthritis.

Synoviolin 1 (SYVN1) plays a vital role in endoplasmic reticulum (ER)-associated degradation. The encoded protein acts as an E3 ubiquitin ligase and suppresses endoplasmic reticulum stress. It might also be involved in the protein quality control of optineurin (OPTN). Upregulated expression of the protein enhanced tau cytotoxicity and promoted cell survival in a study. SYVN1 interacts with Pael -R (substrate protein of familial Parkinson′s disease responsible gene Parkin) to promote neuronal cell death. It also increases degradation of APP (precursor of Alzheimer′s disease-causing protein amyloid β). SYVN1 activity is induced via inositol-requiring enzyme 1-X-box binding protein 1 (IRE1-XBP1) pathway. Therefore, drugs that enhance or suppress the expression of SYVN1, can be used as a therapeutic drugs for neurodegenerative diseases.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	ABS1623	SAB4200552	SAB4200337
Sigma-Aldrich SAB4200423 Anti-HRD1/SYVN1 antibody, Mouse monoclonal	Sigma-Aldrich ABS1623 Anti-HRD1 Antibody	Sigma-Aldrich SAB4200552 Anti-WASH1 antibody, Mouse monoclonal	Sigma-Aldrich SAB4200337 Anti-phospho-RasGRP1(pThr¹⁸⁴) antibody, Mouse monoclonal
description clone HRD1-5, purified from hybridoma cell culture	description from rabbit, purified by affinity chromatography	description clone WASH1-27, purified from hybridoma cell culture	description clone p-RasGRP1-1, purified from hybridoma cell culture
species reactivity human	species reactivity mouse, human, rat	species reactivity mouse, human	species reactivity human
clone HRD1-5, monoclonal	clone polyclonal	clone WASH1-27, monoclonal	clone p-RasGRP1-1, monoclonal
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
antibody form purified from hybridoma cell culture	antibody form purified antibody	antibody form purified from hybridoma cell culture	antibody form purified from hybridoma cell culture
Gene Information human ... SYVN1(84447)	Gene Information human ... SYVN1(84447)	Gene Information human ... WASH1(100287171)	Gene Information human ... RASGRP1(10125) mouse ... Rasgrp1(19419) rat ... Rasgrp1(29434)
biological source mouse	biological source rabbit	biological source mouse	biological source mouse

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Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c