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RANK Ligand/TRANCE human

Name: RANK Ligand/TRANCE human
Brand: SIGMA
Price: 800 USD
Availability: InStock

>90% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder

Synonym(s):

Osteoclast Differentiation Factor (ODF), Osteoprotegerin Ligand (OPGL), Receptor Activator of NF-KB Ligand (RANKL), TNF-related activation-induced cytokines (TRANCE)

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Size/SKU	Availability	Price
10 μg	Check Cart for Availability	$800.00

About This Item

CAS Number:

207621-35-0

UNSPSC Code:

12352202

NACRES:

NA.32

MDL number:

MFCD01867142

Form:

lyophilized powder

Assay:

>90% (SDS-PAGE)

Biological source:

human

Recombinant:

expressed in NSO cells

Mol wt:

glycolysylated recombinant product ~35 kDa by SDS-PAGE, calculated mol wt 23 kDa

$800.00

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Properties

biological source

human

Quality Segment

200

recombinant

expressed in NSO cells

assay

>90% (SDS-PAGE)

form

lyophilized powder

potency

1.5-7.5 ng/mL ED₅₀

mol wt

glycolysylated recombinant product ~35 kDa by SDS-PAGE, calculated mol wt 23 kDa

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

impurities

endotoxin, tested

UniProt accession no.

O14788

application(s)

cell analysis

storage temp.

−20°C

Gene Information

human ... TNFSF11(8600)

Description

General description

Tumor necrosis factor (ligand) superfamily, member 11 (Tnfsf11) is also known as receptor activator of nuclear factor-κB ligand (RANKL). It is a type II transmembrane signaling receptor. It has a molecular weight of around 35kDa. This protein contains an amino-terminal intracellular tail and a carboxy-terminal extracellular region, that bears a connecting stalk and a receptor-binding domain. Tnfsf11 is located on human chromosome 13q14.11.

Application

RANK Ligand/TRANCE human can be used in in vitro osteoclastogenic assays for osteoclast differentiation.

RANK Ligand/TRANCE human has been used to stimulate differentiation.

Biochem/physiol Actions

RANK Ligand (receptor activator of NF-kB ligand (RANKL) also referred to as TNF-related activation induced cytokines (TRANCE) or osteoprotegerin ligand is a protein that belongs to tumor necrosis factor (TNF) family. It stimulates the mature dendritic cells and induces the cytokine production. RANKL also facilitates bone remodelling and possess an angiogenic activity.

Member of the TNF superfamily; induces activation of the c-jun N-terminal kinase, enhances T-cell growth and dendritic cell function, induces osteoclastogenesis, and lymph node organogenesis; cell surface receptor for RANK Ligand.

RANK Ligand/TRANCE, a member of the TNF superfamily, induces activation of the c-jun N-terminal kinase, enhances T-cell growth and dendritic cell function, induces osteoclastogenesis, and lymph node organogenesis. Mouse and human RANK Ligand share 85% amino acid identity. RANK is the cell surface receptor for RANK Ligand.

Physical form

Lyophilized from a 0.2 μm filtered solution in 20 mM MOPS and 500 mM NaCl, pH 6.5, with 50 μg BSA per 1 μg as a carrier protein.

Analysis Note

The biological activity is measured by its ability to induce osteoclast differentiation on mouse splenocytes.

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This Item	GF091	R0525	T5694
Sigma-Aldrich T3573 RANK Ligand/TRANCE human	Sigma-Aldrich GF091 Soluble RANK Ligand (sRANKL) Protein, Recombinant human	Sigma-Aldrich R0525 RANK Ligand from mouse	Sigma-Aldrich T5694 TRAIL human
description >90% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder	description The recombinant human sRANKL protein is produced in E. Coli.	description ≥98% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture	description recombinant, expressed in NSO cells, >97% (SDS-PAGE), lyophilized powder
assay >90% (SDS-PAGE)	assay >98% (SDS-PAGE and HPLC)	assay ≥98% (SDS-PAGE)	assay >97% (SDS-PAGE)
biological source human	biological source human	biological source mouse	biological source human
recombinant expressed in NSO cells	recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in NSO cells
form lyophilized powder	form -	form lyophilized powder	form lyophilized powder
mol wt glycolysylated recombinant product ~35 kDa by SDS-PAGE, calculated mol wt 23 kDa	mol wt -	mol wt -	mol wt 24 kDa by SDS-PAGE, predicted mol wt ~21 kDa
storage temp. −20°C	storage temp. -10 to -25°C	storage temp. −20°C	storage temp. −20°C

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

signalword

Danger

hcodes

EUH451

pcodes

P201 - P202 - P273 - P391 - P501

Hazard Classifications

vPvM

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