Z-2093
ZytoLight® SPEC ERBB2/TOP2A/CEN 17 Triple Color Probe
green to orange, Blue, pkg of 0.05 mL, pkg of 0.2 mL
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packaging
pkg of 0.05 mL, pkg of 0.2 mL, pkg of 20 tests, pkg of 5 tests
storage condition
protect from light
color
green to orange, Blue
application(s)
research use
storage temp.
2-8°C
General description
The ZytoLight® SPEC ERBB2/TOP2A/CEN 17 Triple Color Probe (PL52) is a direct-labeled FISH probe designed for the qualitative detection of amplifications involving the human ERBB2 and TOP2A genes, as well as the detection of chromosome 17 alpha satellites in formalin-fixed, paraffin-embedded (FFPE) specimens. It is intended to be used with the ZytoLight FISH-Tissue Implementation Kit.
Principle:
The fluorescence in situ hybridization (FISH) technique enables the detection and visualization of specific nucleic acid sequences. Fluorescently-labeled DNA fragments (FISH probes) co-denature and hybridize with complementary target DNA strands, followed by stringent washing to remove unbound fragments. The hybridized probes are visualized using a fluorescence microscope equipped with specific excitation and emission filters for the fluorochromes.In this assay, 10 µl of the probe is applied to pretreated formalin-fixed, paraffin-embedded (FFPE) sections. The sections are denatured for 10 minutes at 75 °C, hybridized overnight at 37 °C, and then washed and examined to detect signals: green for ERBB2, orange for TOP2A, and blue for CEN 17. The use of directly labeled DNA probes streamlines the process by eliminating the need for antibody detection.
Components:
ZytoLight® SPEC ERBB2/TOP2A/CEN 17 Triple Color Probe comprises:
Principle:
The fluorescence in situ hybridization (FISH) technique enables the detection and visualization of specific nucleic acid sequences. Fluorescently-labeled DNA fragments (FISH probes) co-denature and hybridize with complementary target DNA strands, followed by stringent washing to remove unbound fragments. The hybridized probes are visualized using a fluorescence microscope equipped with specific excitation and emission filters for the fluorochromes.In this assay, 10 µl of the probe is applied to pretreated formalin-fixed, paraffin-embedded (FFPE) sections. The sections are denatured for 10 minutes at 75 °C, hybridized overnight at 37 °C, and then washed and examined to detect signals: green for ERBB2, orange for TOP2A, and blue for CEN 17. The use of directly labeled DNA probes streamlines the process by eliminating the need for antibody detection.
Components:
ZytoLight® SPEC ERBB2/TOP2A/CEN 17 Triple Color Probe comprises:
- ZyGreen labeled polynucleotides (excitation 503 nm/emission 528 nm) targeting the ERBB2 gene region on chromosome 17 (17q12-q21.1).
- ZyOrange labeled polynucleotides (excitation 547 nm/emission 572 nm) targeting the TOP2A gene region on chromosome 17 (17q21.1-q21.2).
- ZyBlue labeled polynucleotides (excitation 418 nm/emission 467 nm) targeting the alpha satellite centromeric region D17Z1 of chromosome 17.
- Formamide-based hybridization buffer.
Application
For the qualitative detection of human ERBB2 gene amplifications, human TOP2A gene amplifications and alpha satellites of chromosome 17 by fluorescence in situ hybridization (FISH)
Legal Information
ZytoLight is a registered trademark of ZytoVision GmbH in Germany
Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Carc. 2 - Repr. 1B - STOT RE 2 Oral
Target Organs
Blood
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
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